pLV3-CMV-Cxcl13-CopGFP-Puro pl

pLV3-CMV-Cxcl13-CopGFP-Puro plasmid

PVT96628

pLV3-CMV-Cxcl13-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Cxcl13(mouse)-CopGFP-Puro
Alias: NM_018866.3;4631412M08Rik; Angie; ANGIE2; BCA-1; BLC;BLR1L; Scyb13
Gene lenght: 330bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Cxcl13-CopGFP-Puro plasmid Description

pLV3-CMV-Cxcl13-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Cxcl13-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga ggctcagcac agcaacgctg 2341 cttctcctcc tggccagctg cctctctcca ggccacggta ttctggaagc ccattacaca 2401 aacttaaaat gtaggtgttc tggagtgatt tcaactgttg tcggtctaaa catcatagat 2461 cggattcaag ttacgccccc tgggaatggc tgccccaaaa ctgaagttgt gatctggacc 2521 aagatgaaga aagttatatg tgtgaatcct cgtgccaaat ggttacaaag attattaaga 2581 catgtccaaa gcaaaagtct gtcttcaact ccccaagctc cagtgagtaa gagaagagct 2641 gcctgaggat ccCTGCGATA TCCAGCgcgg ccgcGaagga tctgcgatcg ctccggtgcc 2701 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 2761 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 2821 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 2881 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 2941 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 3001 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 3061 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 3121 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 3181 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 3241 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 3301 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 3361 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 3421 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 3481 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 3541 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 3601 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 3661 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 3721 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 3781 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 3841 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 3901 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 3961 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 4021 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 4081 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 4141 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 4201 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 4261 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 4321 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 4381 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 4441 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 4501 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 4561 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 4621 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 4681 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 4741 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 4801 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 4861 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 4921 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 4981 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 5041 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 5101 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 5161 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg 5221 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 5281 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 5341 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 5401 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 5461 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 5521 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 5581 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 5641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 5701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 5881 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 5941 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6001 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6061 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6121 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6481 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6541 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6601 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6661 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 6721 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 6781 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 6841 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 6901 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 6961 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7021 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7081 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7141 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7201 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7261 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7321 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7381 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7441 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7501 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7561 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7621 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 7681 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 7741 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 7801 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 7861 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 7921 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 7981 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8041 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 8101 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 8161 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 8221 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 8281 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 8341 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 8401 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 8461 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 8521 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.