pTac-GST-Atp6v1a plasmid

pTac-GST-Atp6v1a plasmid

PVT96565

pTac-GST-Atp6v1a plasmid Information

Plasmid name: pTac-GST-Atp6v1a(mouse)
Alias: NM_001358204.1;Atp6a1; Atp6a2; Atp6v1a1; VA68; VPP2
Gene lenght: 1854bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction:

pTac-GST-Atp6v1a plasmid Description

pTac-GST-Atp6v1a is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-Atp6v1a plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatgga tttctccaag ctacccaaaa 961 tccgagatga ggataaagaa agtacatttg gttatgtgca tggagtctca gggcctgtgg 1021 ttacagcctg tgacatggcg ggcgctgcca tgtacgagct ggtgagagtg gggcacagcg 1081 agctggttgg agaaattatt cgattggaag gtgacatggc caccattcag gtgtatgaag 1141 aaacttctgg tgtctctgtt ggagaccccg tactccgcac tggtaaacct ctctcggtcg 1201 agctgggtcc cgggattatg ggagccattt ttgatggtat acagagacct ctgtcggata 1261 tcagcagtca gacccaaagt atctacatcc ccagaggagt caatgtgtct gctctcagca 1321 gagatatcaa atgggagttt atacccagca aaaacctacg ggttggtagt catatcactg 1381 gtggagacat ttatgggatt gtcaatgaga actccctcat caaacacaaa atcatgttgc 1441 ccccacgtaa cagaggaagc gtgacttaca tcgcgccgcc tgggaattat gatgcatccg 1501 atgtcgtcct ggagcttgag tttgaaggtg tgaaggagaa gttcagcatg gtccaagtgt 1561 ggcctgtgcg gcaggtgcgg cctgtcactg agaagctgcc cgccaatcac cccttgctta 1621 ctggccagag agtcctcgat gcccttttcc cgtgtgttca gggaggaact actgctatcc 1681 caggcgcctt tggctgtgga aagactgtga tttcccagtc tctatccaag tactccaaca 1741 gtgacgtcat catctatgtc ggctgcggtg agagaggcaa cgagatgtca gaagttctcc 1801 gcgacttccc tgagctcacc atggaggttg atgggaaagt agagtccatc atgaagagga 1861 cagcgctggt agccaacacc tccaacatgc ctgtggctgc gagagaggcc tccatctaca 1921 ctggaattac actatcagaa tatttccgtg acatgggcta ccacgtcagt atgatggccg 1981 actctacctc tagatgggct gaggccctca gagaaatctc tggtcgctta gctgagatgc 2041 ctgcagatag tggataccct gcataccttg gtgcccggct ggcttctttc tatgagcgag 2101 caggcagagt gaaatgtctc ggaaaccctg agagagaagg gagtgtcagc attgtaggag 2161 cagtttctcc acctggtggt gatttttctg atccagtcac ttctgcaacg ctgggtattg 2221 ttcaggtgtt ctggggcttg gataagaagt tagctcagcg caagcacttc ccctctgtca 2281 actggctcat cagctacagc aagtacatgc gtgccctgga cgagtactat gacaagcact 2341 tcacggagtt tgttcctctg aggaccaaag ctaaggagat cctgcaggaa gaagaggatc 2401 tggcagaaat cgtgcagctc gtgggaaagg cctctttagc agagacggat aaaatcactc 2461 tggaggtagc aaaacttatt aaagatgact tcctacagca aaatgggtac actccttatg 2521 acaggttctg tccattctac aagacagtgg ggatgctgtc caacatgatt tcattctatg 2581 acatggcccg ccgagctgtg gagaccactg cccagagtga caataagatc acatggtcca 2641 ttatccggga gcacatgggg gagattctct ataaactctc ctccatgaaa ttcaaggatc 2701 cagtgaaaga tggcgaggca aagatcaagg ccgactatgc acagcttctt gaagatatgc 2761 agaatgcatt ccgtagcctt gaagactagc tcgagcggcc gcatcgtgac tgactgacga 2821 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 2881 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 2941 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 3001 ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 3061 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 3121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 3181 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 3241 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 3301 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 3361 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 3421 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg 3481 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 3541 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 3601 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 3661 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 3721 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca 3781 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 3841 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 3901 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 3961 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 4021 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 4081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 4141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 4201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 4261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 4321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 4381 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 4801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 4861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 4921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 4981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta 5041 cgcatctgtg cggtatttca caccgcataa attccgacac catcgaatgg tgcaaaacct 5101 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac 5161 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 5221 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 5281 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 5341 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 5401 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 5461 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 5521 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 5581 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 5641 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 5701 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 5761 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 5821 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 5881 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 5941 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 6001 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 6061 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 6121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 6181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 6241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 6301 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 6361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 6421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 6481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt 6541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga 6601 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta 6661 caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac 6721 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg 6781 aattattttt gatggcgttg gaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.