pT3-GFAP-MCS-3×Flag-P2A-EGFP p

pT3-GFAP-MCS-3×Flag-P2A-EGFP plasmid

PVT96547

pT3-GFAP-MCS-3×Flag-P2A-EGFP plasmid Information

Plasmid name: pT3-GFAP-MCS-3×Flag-P2A-EGFP
Alias: -
Gene lenght:
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: Non
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: PEF-F
Reverse primer:
Copy number: High
Induction:

pT3-GFAP-MCS-3×Flag-P2A-EGFP plasmid Description

pT3-GFAP-MCS-3×Flag-P2A-EGFP is a plasmid suitable for protein expression in mammalian cells.

pT3-GFAP-MCS-3×Flag-P2A-EGFP plasmid Sequence

1 GACTAATTGA CATCATTTGA GTCAATTGGA GGTGTACCTG TGGATGTATT TCAAGGAATT 61 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 121 ATGCAAAGCA TGCATATCGA TACTAGTgcg gccgcacgcg tgatctaaca tatcctggtg 181 tggagtaggg gacgctgctc tgacagaggc tcgggggcct gagctggctc tgtgagctgg 241 ggaggaggca gacagccagg ccttgtctgc aagcagacct ggcagcattg ggctggccgc 301 cccccagggc ctcctcttca tgcccagtga atgactcacc ttggcacaga cacaatgttc 361 ggggtgggca cagtgcctgc ttcccgccgc accccagccc ccctcaaatg ccttccgaga 421 agcccattga gcagggggct tgcattgcac cccagcctga cagcctggca tcttgggata 481 aaagcagcac agccccctag gggctgccct tgctgtgtgg cgccaccggc ggtggagaac 541 aaggctctat tcagcctgtg cccaggaaag gggatcaggg gatgcccagg catggacagt 601 gggtggcagg gggggagagg agggctgtct gcttcccaga agtccaagga cacaaatggg 661 tgaggggaga gctctcccca tagctgggct gcggcccaac cccaccccct caggctatgc 721 cagggggtgt tgccaggggc acccgggcat cgccagtcta gcccactcct tcataaagcc 781 ctcgcatccc aggagcgagc agagccagag caggttggag aggagacgca tcacctccgc 841 tgctcgcggg gtctagagtc gacgccaccG ATATCCAGCA CAGTGGCGCT CGAGCGTCTA 901 GAGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG 961 ATGACAAGGC TACTAACTTC AGCCTGCTGA AGCAGGCTGG AGACGTGGAG GAGAACCCTG 1021 GACCTGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG 1081 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG 1141 ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC 1201 CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG 1261 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC 1321 GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG 1381 GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA 1441 TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA 1501 AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG 1561 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC 1621 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG 1681 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC 1741 TGTACAAGTA AGCGGCCGCT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT 1801 TATCATGTCT GGATCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT 1861 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC 1921 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT 1981 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT 2041 GCGGTGGGCT CTATGGCTTC TGAGGCGGGA TCCCCTTGAA ATACATCCAC AGGTACACCT 2101 CCAATTGACT CAAATGATGT CAATTAGTCT ATCAGAAGCT TCTAAAGCCA TGACATCATT 2161 TTCTGGAATT TTCCAAGCTG TTTAAAGGCA CAGTCAACTT AGTGTATGTA AACTTCTGAC 2221 CCACTGGAAT TGTGATACAG TGAATTATAA GTGAAATAAT CTGTCTGTAA ACAATTGTTG 2281 GAAAAATGAC TTGTGTCATG CACAAAGTAG ATGTCCTAAC TGACTTGCCA AAACTATTGT 2341 TTGTTAACAA GAAATTTGTG GAGTAGTTGA AAAACGAGTT TTAATGACTC CAACTTAAGT 2401 GTATGTAAAC TTCCGACTTC AACTGTATTC GACCTGCAGG CATGCAAGCT TGGCGTAATC 2461 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 2521 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 2581 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 2641 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT 2701 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 2761 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 2821 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 2881 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 2941 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 3001 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 3061 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 3121 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 3181 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 3241 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 3301 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 3361 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 3421 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 3481 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 3541 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 3601 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC 3661 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT 3721 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC 3781 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC 3841 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG 3901 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG 3961 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG 4021 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG 4081 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT 4141 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA 4201 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC 4261 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC 4321 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC 4381 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC 4441 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA 4501 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 4561 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT 4621 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT 4681 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 4741 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 4801 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 4861 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 4921 GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC 4981 GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC 5041 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC TCGGTACCCT 5101 ACAGTTGAAG TCGGAAGTTT ACATACACTT AAGTTGGAGT CATTAAAACT CGTTTTTCAA 5161 CTACTCCACA AATTTCTTGT TAACAAACAA TAGTTTTGGC AAGTCAGTTA GGACATCTAC 5221 TTTGTGCATG ACACAAGTCA TTTTTCCAAC AATTGTTTAC AGACAGATTA TTTCACTTAT 5281 AATTCACTGT ATCACAATTC CAGTGGGTCA GAAGTTTACA TACACTAAGT TGACTGTGCC 5341 TTTAAACAGC TTGGAAAATT CCAGAAAATG ATGTCATGGC TTTAGAAGCT TCTGATA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.