pCMV-3×Myc-Lrrfip1-Neo plasmid

pCMV-3×Myc-Lrrfip1-Neo plasmid

PVT96545

pCMV-3×Myc-Lrrfip1-Neo plasmid Information

Plasmid name: pCMV-3×Myc-Lrrfip1(mouse)-Neo
Alias: NM_001111311.1;Fliiap1
Gene lenght: 2190bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×Myc-Lrrfip1-Neo plasmid Description

pCMV-3×Myc-Lrrfip1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×Myc-Lrrfip1-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGGAA CAAAAACTAA TATCAGAGGA AGATTTGGAA 961 CAGAAACTAA TCAGTGAAGA AGATTTAGAG CAAAAGTTAA TTTCCGAGGA GGACTTATCA 1021 GCGGAAAAGC TTatgaccag cccagagggc gcgcaaaaca aggaaatcga ctgtctgagt 1081 ccagaggcgc aaagactggc ggaggccagg ctcgcagcga agcgtgcagc ccgagccgag 1141 gctcgagaga tccggatgaa ggagctggaa cggcagcaga aagaggtaga agaaagacca 1201 gacaaagact ttgctgagaa gggctcccgt aacatgccta gcctgtctgc agccacactg 1261 gcatctctgg gtgggacttc ctcccggaga gggagtggag atacctccat ctccatggac 1321 accgaagcat ccatcaggga aatcaaggac tcgctggccg aagttgagga gaagtacaag 1381 aaagctatgg tctccaacgc ccagctagac aatgagaaga ccaacttcat gtaccaggtg 1441 gacacactga aggacatgct gctggagctg gaagagcagc tggccgagtc tcagcggcaa 1501 tacgaggaga agaacaagga gttcgagagg gagaagcatg cccacagcat cctgcagttc 1561 cagtttgccg aagtgaaaga ggccctgcgg caaagggaag agatgctcga gaaacatgga 1621 ataatcctaa attcagaaat agccaccaac ggagagactt cagacacagt aaatgacgtt 1681 gggtatcaag cccctacgaa gataacaaaa gaagagctga atgccctcaa gtcggccggg 1741 gaggggaccc taggaaaagc caaagaagtg gaggtgaaaa aggaaattgt ggagaaagtg 1801 gggcaaagag aaaccttaca gaattctgag caagaacagc ccaaacccaa cacaggaaag 1861 gattgtgtgg acagaggggt gtcacatcct ggtgagaagg ctgagaacca gagacccgct 1921 gaagacagtg ccctgtcccc gggaccacta gcaggggcca aatgtgagca acaggttcag 1981 agccaagatc aagagaatac ttctgacctc aaaaactcag agcagatcga gtcacacaag 2041 gtcacaaaca agtcagatag tagggccagt aactcccctg aacagtccag ctgcctggaa 2101 ggtctagaca gtgaagtgcc agggcccact gaggatttga aaacagattt ggggaaaggt 2161 agctttgaac catgtccaga ctacattttg gggcaaacag ctgaaattga caaggtgacc 2221 tgtacagact caagaggcac aggtggaaac caaagggagg atgaggtaca ggcaggggac 2281 accacagttg aggaccaagt agggacagtg gcttctggac ctgcaaagca aagcaaaggc 2341 accgagaatc atggtgaaag ttgtctgaag gatgggctgg gacagagctc agagagagaa 2401 ctcacccaag aagtagctga gcctgaggaa gccatcgttc agatcccaca ggccggtgga 2461 gagaacacta ttaccaaggc agatgatgcc gaaggaaggg atgagaagcc catccaggca 2521 gaagcccagg caagccctgg agctcccata aatcagagcg gccatcagga cacaacagga 2581 ccaggcagca ccgatgccca gcgcacacca cctcatgcaa aagagcgcaa gaaacaaggg 2641 aagagcgagc agcaggcaga ggccttggat tcaccgcaga agaagacgaa gaacaagaag 2701 aagaagaaca agaagaagaa agctgcaact cccgcggaaa cctgcagaga tgccaatgaa 2761 gagttgaact gtcaggaccc agatgtgggt gacatggagg aggaagagcg gctccaggtc 2821 actgacaaga aacaggcatc aggaagccca gagcagaaaa tcagagcggg aagcagagaa 2881 cctgtcgaag atcctcaaag tgggtcgagt ggaaaacaga acaaagtgga agaggatggt 2941 cccacggaag gtcccacaga catcttggat cagaacagtc cccagtgtga agacagggag 3001 atctccccag tgggagagaa aggtccccaa tgcgatacct ctcagatagg tagtgaagag 3061 ggacatgtga cctcccaaca cggtggtcag gcagttgaga accacaacct agataacagt 3121 gacctgtcag ggcagctgga gggcttcaat tcagaaagtg gagggcaggc aagagaggag 3181 gttgggaata gcaagagtaa ggaggactgc accatgtcgt gagaattctg cagatatcca 3241 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact 3301 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 3361 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 3421 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 3481 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 3541 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 3601 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 3661 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 3721 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 3781 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 3841 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 3901 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 3961 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 4021 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 4081 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 4141 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 4201 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 4261 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 4321 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 4381 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 4441 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 4501 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 4561 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 4621 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 4681 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 4741 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 4801 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 4861 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 4921 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 4981 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 5041 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 5101 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 5161 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 5221 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 5281 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 5341 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 5401 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5461 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 5521 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 5581 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 5641 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 5701 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 5761 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 5821 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 5881 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 5941 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6001 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6061 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6121 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6181 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6241 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6301 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6361 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 6421 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6481 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6541 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6601 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6661 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 6721 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 6781 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 6841 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 6901 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 6961 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 7021 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7081 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7141 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7201 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7261 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 7321 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7381 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7441 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7501 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7561 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7621 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7681 aaaagtgcca cctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.