pCMV-Tlr7-3×FLAG-Neo plasmid

pCMV-Tlr7-3×FLAG-Neo plasmid

PVT96524

pCMV-Tlr7-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Tlr7(mouse)-3×FLAG-Neo
Alias: NM_001290755.1
Gene lenght: 3150bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Tlr7-3×FLAG-Neo plasmid Description

pCMV-Tlr7-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Tlr7-3×FLAG-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT GGAATTCGCC 961 ACCatggtgt tttcgatgtg gacacggaag agacaaattt tgatcttttt aaatatgctc 1021 ttagtttcta gagtctttgg gtttcgatgg tttcctaaaa ctctaccttg tgaagttaaa 1081 gtaaatatcc cagaggccca tgtgatcgtg gactgcacag acaagcattt gacagaaatc 1141 cctgagggca ttcccactaa caccaccaat cttaccctta ccatcaacca cataccaagc 1201 atctctccag attccttccg taggctgaac catctggaag aaatcgattt aagatgcaat 1261 tgtgtacctg ttctactggg gtccaaagcc aatgtgtgta ccaagaggct gcagattaga 1321 cctggaagct ttagtggact ctctgactta aaagcccttt acctggatgg aaaccaactt 1381 ctggagatac cacaggatct gccatccagc ttacatcttc tgagccttga ggctaacaac 1441 atcttctcca tcacgaagga gaatctaaca gaactggtca acattgaaac actctacctg 1501 ggtcaaaact gttattatcg aaatccttgc aatgtttcct attctattga aaaagatgct 1561 ttcctagtta tgagaaattt gaaggttctc tcactaaaag ataacaatgt cacagctgtc 1621 cccaccactt tgccacctaa tttactagag ctctatcttt ataacaatat cattaagaaa 1681 atccaagaaa atgattttaa taacctcaat gagttgcaag ttcttgacct aagtggaaat 1741 tgccctcgat gttataatgt cccatatccg tgtacaccgt gtgaaaataa ttccccctta 1801 cagatccatg acaatgcttt caattcattg acagaattaa aagttttacg tttacacagt 1861 aattctcttc agcatgtgcc cccaacatgg tttaaaaaca tgagaaacct ccaggaacta 1921 gacctctccc aaaactactt ggccagagaa attgaggagg ccaaattttt gcattttctt 1981 cccaaccttg ttgagttgga tttttctttc aattatgagc tgcaggtcta ccatgcatct 2041 ataactttac cacattcact ctcttcattg gaaaacttga aaattctgcg tgtcaagggg 2101 tatgtcttta aagagctgaa aaactccagt ctttctgtat tgcacaagct tcccaggctg 2161 gaagttcttg accttggcac taacttcata aaaattgctg acctcaacat attcaaacat 2221 tttgaaaacc tcaaactcat agacctttca gtgaataaga tatctccttc agaagagtca 2281 agagaagttg gcttttgtcc taatgctcaa acttctgtag accgtcatgg gccccaggtc 2341 cttgaggcct tacactattt ccgatacgat gaatatgcac ggagctgcag gttcaaaaac 2401 aaagagccac cttctttctt gcctttgaat gcagactgcc acatatatgg gcagacctta 2461 gacttaagta gaaataacat attttttatt aaaccttctg attttcagca tctttcattc 2521 ctcaaatgcc tcaacttatc aggaaacacc attggccaaa ctcttaatgg cagtgaactc 2581 tggccgttga gagagttgcg gtacttagac ttctccaaca accggcttga tttactctac 2641 tcaacagcct ttgaagagct ccagagtctt gaagttctgg atctaagtag taacagccac 2701 tattttcaag cagaaggaat tactcacatg ctaaacttta ccaagaaatt acggcttctg 2761 gacaaactca tgatgaatga taatgacatc tctacttcgg ccagcaggac catggaaagt 2821 gactctcttc gaattctgga gttcagaggc aaccatttag atgttctatg gagagccggt 2881 gataacagat acttggactt cttcaagaat ttgttcaatt tagaggtatt agatatctcc 2941 agaaattccc tgaattcctt gcctcctgag gtttttgagg gtatgccgcc aaatctaaag 3001 aatctctcct tggccaaaaa tgggctcaaa tctttctttt gggacagact ccagttactg 3061 aagcatttgg aaattttgga cctcagccat aaccagctga caaaagtacc tgagagattg 3121 gccaactgtt ccaaaagtct cacaacactg attcttaagc ataatcaaat caggcaattg 3181 acaaaatatt ttctagaaga tgctttgcaa ttgcgctatc tagacatcag ttcaaataaa 3241 atccaggtca ttcagaagac tagcttccca gaaaatgtcc tcaacaatct ggagatgttg 3301 gttttacatc acaatcgctt tctttgcaac tgtgatgctg tgtggtttgt ctggtgggtt 3361 aaccatacag atgttactat tccatacctg gccactgatg tgacttgtgt aggtccagga 3421 gcacacaaag gtcaaagtgt catatccctt gatctgtata cgtgtgagtt agatctcaca 3481 aacctgattc tgttctcagt ttccatatca tcagtcctct ttcttatggt agttatgaca 3541 acaagtcacc tctttttctg ggatatgtgg tacatttatt atttttggaa agcaaagata 3601 aaggggtatc agcatctgca atccatggag tcttgttatg atgcttttat tgtgtatgac 3661 actaaaaact cagctgtgac agaatgggtt ttgcaggagc tggtggcaaa attggaagat 3721 ccaagagaaa aacacttcaa tttgtgtcta gaagaaagag actggctacc aggacagcca 3781 gttctagaaa acctttccca gagcatacag ctcagcaaaa agacagtgtt tgtgatgaca 3841 cagaaatatg ctaagactga gagttttaag atggcatttt atttgtctca tcagaggctc 3901 ctggatgaaa aagtggatgt gattatcttg atattcttgg aaaagcctct tcagaagtct 3961 aagtttcttc agctcaggaa gagactctgc aggagctctg tccttgagtg gcctgcaaat 4021 ccacaggctc acccatactt ctggcagtgc ctgaaaaatg ccctgaccac agacaatcat 4081 gtggcttata gtcaaatgtt caaggaaaca gtcCTCGAGG ACTACAAAGA CCATGACGGT 4141 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGTG AGGGCCCGTT 4201 TAAACCCGCT GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC 4261 TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT 4321 GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG 4381 CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC 4441 TCTATGGCTT CTGAGGCGGA AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC 4501 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 4561 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 4621 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 4681 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 4741 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 4801 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 4861 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 4921 TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA 4981 GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT 5041 CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC 5101 CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG 5161 GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC 5221 AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT 5281 GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC 5341 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT 5401 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC 5461 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG 5521 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG 5581 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT 5641 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC 5701 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG 5761 CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG 5821 GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC 5881 TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT 5941 CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG 6001 CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT 6061 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT 6121 TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG 6181 AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA 6241 CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA 6301 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 6361 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 6421 TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT 6481 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 6541 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 6601 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC 6661 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 6721 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 6781 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 6841 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 6901 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 6961 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 7021 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 7081 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 7141 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 7201 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 7261 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 7321 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 7381 CGGCAAACAA ACCACCGCTG GTAGCGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 7441 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 7501 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 7561 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC 7621 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC 7681 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC 7741 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 7801 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC 7861 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT 7921 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC 7981 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA 8041 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 8101 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG 8161 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC 8221 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA 8281 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT 8341 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 8401 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG 8461 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA 8521 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT 8581 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.