pLV2-EF1a-Myc-Xpo5-IRES-Puro p

pLV2-EF1a-Myc-Xpo5-IRES-Puro plasmid

PVT96480

pLV2-EF1a-Myc-Xpo5-IRES-Puro plasmid Information

Plasmid name: pLV2-EF1a-Myc-Xpo5(mouse)-IRES-Puro
Alias: NM_028198.3;2410004H11Rik; 2700038C24Rik; Exp5;mKIAA1291; RanBp21
Gene lenght: 3615bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF-1α-F1
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Myc-Xpo5-IRES-Puro plasmid Description

pLV2-EF1a-Myc-Xpo5-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-EF1a-Myc-Xpo5-IRES-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATGAGGCC CTTTCGTCTT CACTCGAGGT GCCCGTCAGT 2221 GGGCAGAGCG CACATCGCCC ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA 2281 CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC 2341 GCCTTTTTCC CGAGGGTGGG GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC 2401 TTTTTCGCAA CGGGTTTGCC GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC 2461 CTGGCCTCTT TACGGGTTAT GGCCCTTGCG TGCCTTGAAT TACTTCCACC TGGCTGCAGT 2521 ACGTGATTCT TGATCCCGAG CTTCGGGTTG GAAGTGGGTG GGAGAGTTCG AGGCCTTGCG 2581 CTTAAGGAGC CCCTTCGCCT CGTGCTTGAG TTGAGGCCTG GCCTGGGCGC TGGGGCCGCC 2641 GCGTGCGAAT CTGGTGGCAC CTTCGCGCCT GTCTCGCTGC TTTCGATAAG TCTCTAGCCA 2701 TTTAAAATTT TTGATGACCT GCTGCGACGC TTTTTTTCTG GCAAGATAGT CTTGTAAATG 2761 CGGGCCAAGA TCTGCACACT GGTATTTCGG TTTTTGGGGC CGCGGGCGGC GACGGGGCCC 2821 GTGCGTCCCA GCGCACATGT TCGGCGAGGC GGGGCCTGCG AGCGCGGCCA CCGAGAATCG 2881 GACGGGGGTA GTCTCAAGCT GGCCGGCCTG CTCTGGTGCC TGGCCTCGCG CCGCCGTGTA 2941 TCGCCCCGCC CTGGGCGGCA AGGCTGGCCC GGTCGGCACC AGTTGCGTGA GCGGAAAGAT 3001 GGCCGCTTCC CGGCCCTGCT GCAGGGAGCT CAAAATGGAG GACGCGGCGC TCGGGAGAGC 3061 GGGCGGGTGA GTCACCCACA CAAAGGAAAA GGGCCTTTCC GTCCTCAGCC GTCGCTTCAT 3121 GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG AGCTTTTGGA 3181 GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC CACACTGAGT 3241 GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG GAATTTGCCC 3301 TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA AGTTTTTTTC 3361 TTCCATTTCA GGTGTCGTGA GGATCCGCCA CCatgGAACA AAAACTCATC TCAGAAGAGG 3421 ATCTGACGCG Tatggagatg gagcaagtga acgcgctgtg cgaggagcta gtgaaagcgg 3481 tgacggtcat gatggacccc agctctaccc agcgctaccg gctggaagcc ctcaagtttt 3541 gtgaagagtt taaagaaaaa tgccctatct gtgttccatg tggcttgaag ttggctgaaa 3601 aaacacaaat tgccatcgtc agacattttg gccttcagat cctggagcat gttgtcaagt 3661 ttcgatggaa cagcatgtcc cgattggaga aggtctatct gaagaacagt gtcatggagc 3721 tgatcgcaaa tggaacactg aggattttgg aggaggagaa ccacattaaa gatgttctgt 3781 ctagaattgt ggtagagatg atcaaacgag agtggccaca gcactggcct gacatgctca 3841 tggagttgga tactctcttc aggcaagggg aaacgcagag ggagttggtg atgttcatcc 3901 ttctccgact ggccgaggat gtagtgacct ttcagacgct ccccactcaa agaagaaggg 3961 atattcagca aacattgacg cagaacatgg aaagaatctt gaattttcta ctcaatacgc 4021 ttcaggaaaa tgtaaacaag taccaacaaa tgaagacaga ttcatctcag gaggcagagg 4081 ctcaagccaa ctgtcgagta agcgtggcgg ccctgaacac tctagccggc tacattgact 4141 gggtatcttt gaaccatatc actgccgaga actgtaaact cgtggagact ctgtgcctgc 4201 ttctcaatga gcaggagctg cagttgggag ccgccgagtg tctgctcatt gcagtcagca 4261 gaaaaggcaa gctggaggac cggaagcgct tgatgatcct gtttggagat gtcgccatgc 4321 attatattct ctcggccgca cagactgcag atggaggagg cttggtcgaa aaacactacc 4381 tcttcctgaa gagactttgt caggtgttat gtgcactggg aaatctgcta tgtgcactgc 4441 tggccttaga tgctaacata caaacaccta taaactttgg aatgtacctg gaatcttttc 4501 ttgctttcac aacccatcca agtcagtttt tacgctcttc aacccatatg acgtggggag 4561 ccctcttcag gcatgaagtt ctgtcccgtg atccggcact gttagcagta ataccaaaat 4621 atcttcgtgc ttctatgaca aacttggtca agatgggctt tccttctaaa acagatagcc 4681 ccagctgtga atattcccga tttgatttcg atagtgatga ggacttcaat gctttcttca 4741 actcctcccg agcccagcat ggagaggtgg tgaggtgtgt gtgtcgactg gatcctaaga 4801 ctagcttcca gatggcggca gaatggctca agtatcagct gtcagcttct attgacactg 4861 gacctgtgaa ctcatgttct acagctggaa ctggagaggg aggcttctgc tccatcttct 4921 caccttcata tgtgcagtgg gaagctatga cttttttttt ggaaagtgtg atcaaccaga 4981 tgtttcgaac actagacaaa gaagagctcc cggttagtga tgggatagag ctgctgcagc 5041 tggtgttgaa ctttgaaatc aaggatcccc tcgtcctgtc ctgcgtcctc actaatgtct 5101 cagcgctctt cccatttgtc acatacaagc ctgcgttctt gccccaggtc ttctctaagc 5161 tcttttcatt tgttactttt gaatctgttg gggaaagtaa ggcccccagg actcgggcag 5221 tcaggaatgt aaggagacat gcttgttcct ccatcaacaa gatgtgtcgg gactacccag 5281 accttgttct gcccaatttt gacatgcttt atagccacgt gaagcaactc ctctccaatg 5341 agctcctcct gacccagatg gagaagtgcg ccctcatgga agctctggtt ctcgttagca 5401 accaattcaa ggattatgag cggcagaagt tattcctaga ggagctgatg gcaccggtgg 5461 tcaacatctg gctttctgaa gaaatgtgca gagcgctgtc agatattgac tccttcattg 5521 cctatgtggg tgctgatctg aaaagctgtg acccagctgt ggaggatcca tgtggcttga 5581 accgtgcacg aatgagcttt tgtgtgtaca gcatcctggg ggttatgaga cgcactagct 5641 ggccttccga cctagaagaa gccaaagctg ggggctttgt ggtgggctac acacccagtg 5701 gaaatcccat cttccgtaac ccctgcacag agcagatcct cagacttctc gacaatttgc 5761 ttgcccttgt aagaactcac aatactttat atacaccgga aatgctaacg aaaatggcag 5821 aacctttcac caaggctctg gatatagttg aatctgaaaa aacggcaata ttaggattac 5881 ctcagcctct cttggaattc aacgaccacc ctgtctatag aaccactctg gagaggatgc 5941 agcggttctt tggcattctc tatgaaaact gttaccatat cctagggaag gcaggccctt 6001 ccatgcagca agatttctac actgtggagg accttgcttc ccagctcctt ggatcagctt 6061 tcgtcaactt gaacaatatt cctgacttcc ggcttagatc tatgcttcgt gtcttcgtga 6121 agcccctcgt gctcttctgc ccctcagaac actatgaaac cctgatatct cccatccttg 6181 gacctctctt cacctacctc cacatgaggc tctctcagaa gtggcatgtc atcaaccaga 6241 ggagcatcct gtgtggagaa gatgagattg cagaggacaa ccccgagtct caggaaatgc 6301 tcgaggaaca gctggtgagg atgctcaccc gagaagccat ggacctaatc atggcttgct 6361 gtgtgtcgaa gaagactgcc gaccacacag ccgctcccac tgcagatgga gatgatgaag 6421 agatgatggc cactgaagta gccccctcgt ctgtggtgga gctcacagac ctgggcaaat 6481 gcctcatgaa gcacgaggat gtctgcacag cactgctaat cacagcattt aattctctga 6541 cctggaagga cacactgtct tgccagaggg ctaccacaca gctctgctgg cccctcctca 6601 aacaggtgat gtctgggacc ctgctcgcag acgctgtcac ttggcttttc accagtgtgc 6661 tgaaaggact gcagatgcac gggcagcacg atgggtgcat ggcttccctg gtccacttgg 6721 ccttccagat atacgaggcg ctgcgcccca ggtacctaga gataagagca gtaatggagc 6781 agatccctga aataaacaag gagtctctgg accaatttga ctgcaagctt ttaaacccct 6841 cccttcaaaa agcagctgat aaacgacgga aggaccactt caaacgtcta atcgctggct 6901 gcattgggaa acccttggga gaacagttcc gaaaagaagt tcacattaag aaccttccct 6961 ggcttttcaa aaaacccaaa ccaatgttgg agacagaagt gctggacagt gaggagggtg 7021 gactggccac catctttgaa ccctgaGAAT TCCTCGAGGG CGGCCGCTCT AGAGTCGACG 7081 GGCCGCGGTA ACAATTGTTA ACTAACTTAA GCTAGCAACG GTTTCCCTCT AGCGGGATCA 7141 ATTCCGCCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG CCGGTGTGCG 7201 TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG GGCCCGGAAA 7261 CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC CAAAGGAATG 7321 CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG AAGACAAACA 7381 ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG GTGCCTCTGC 7441 GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA GTGCCACGTT 7501 GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT CAACAAGGGG 7561 CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC TCGGTGCACA 7621 TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC 7681 GTGGTTTTCC TTTGAAAAAC ACGATAATAC CATGACCGAG TACAAGCCCA CGGTGCGCCT 7741 CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA 7801 CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA 7861 AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG 7921 CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA 7981 GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA 8041 AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT 8101 CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC 8161 GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT 8221 CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC 8281 CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACCCGGGCGG GGCGCGTCTG GAACAATCAA 8341 CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT 8401 ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT 8461 TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC 8521 GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG 8581 GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC 8641 ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC 8701 ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT 8761 GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA 8821 GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT 8881 CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAT TAATTCTGCA 8941 GTCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG 9001 ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG 9061 TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA 9121 AGAGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA 9181 TCTACCACAC ACAAGGCTAC TTCCCTGATT AGCAGAACTA CACACCAGGG CCAGGGGTCA 9241 GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCCA GATAAGGTAG 9301 AAGAGGCCAA TAAAGGAGAG AACACCAGCT TGTTACACCC TGTGAGCCTG CATGGGATGG 9361 ATGACCCGGA GAGAGAAGTG TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACG 9421 TGGCCCGAGA GCTGCATCCG GAGTACTTCA AGAACTGCTG ATATCGAGCT TGCTACAAGG 9481 GACTTTCCGC TGGGGACTTT CCAGGGAGGC GTGGCCTGGG CGGGACTGGG GAGTGGCGAG 9541 CCCTCAGATC CTGCATATAA GCAGCTGCTT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC 9601 CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA 9661 AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG 9721 AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT 9781 CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGCCTTGA 9841 CATTGCTAGC GTTTACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG 9901 TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA 9961 AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA 10021 CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 10081 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 10141 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 10201 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 10261 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 10321 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 10381 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 10441 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 10501 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 10561 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 10621 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 10681 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA 10741 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 10801 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 10861 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 10921 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 10981 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 11041 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 11101 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 11161 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA 11221 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 11281 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 11341 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 11401 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 11461 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 11521 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 11581 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 11641 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 11701 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 11761 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 11821 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 11881 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 11941 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 12001 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA 12061 GATCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 12121 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 12181 ATCTTATCAT GTCTGGATCA ACTGGATAAC TCAAGCTAAC CAAAATCATC CCAAACTTCC 12241 CACCCCATAC CCTATTACCA CTGCCAATTA CCTGTGGTTT CATTTACTCT AAACCTGTGA 12301 TTCCTCTGAA TTATTTTCAT TTTAAAGAAA TTGTATTTGT TAAATATGTA CTACAAACTT 12361 AGTAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.