pLV3-CMV-CD34-3×FLAG-Puro plas

pLV3-CMV-CD34-3×FLAG-Puro plasmid

PVT96373

pLV3-CMV-CD34-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-CD34(human)-3×FLAG-Puro
Alias: NM_001025109.2
Gene lenght: 1155bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CD34-3×FLAG-Puro plasmid Description

pLV3-CMV-CD34-3×FLAG-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-CD34-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac catgctggtc cgcaggggcg cgcgcgcagg gcccaggatg 2341 ccgcggggct ggaccgcgct ttgcttgctg agtttgctgc cttctgggtt catgagtctt 2401 gacaacaacg gtactgctac cccagagtta cctacccagg gaacattttc aaatgtttct 2461 acaaatgtat cctaccaaga aactacaaca cctagtaccc ttggaagtac cagcctgcac 2521 cctgtgtctc aacatggcaa tgaggccaca acaaacatca cagaaacgac agtcaaattc 2581 acatctacct ctgtgataac ctcagtttat ggaaacacaa actcttctgt ccagtcacag 2641 acctctgtaa tcagcacagt gttcaccacc ccagccaacg tttcaactcc agagacaacc 2701 ttgaagccta gcctgtcacc tggaaatgtt tcagaccttt caaccactag cactagcctt 2761 gcaacatctc ccactaaacc ctatacatca tcttctccta tcctaagtga catcaaggca 2821 gaaatcaaat gttcaggcat cagagaagtg aaattgactc agggcatctg cctggagcaa 2881 aataagacct ccagctgtgc ggagtttaag aaggacaggg gagagggcct ggcccgagtg 2941 ctgtgtgggg aggagcaggc tgatgctgat gctggggccc aggtatgctc cctgctcctt 3001 gcccagtctg aggtgaggcc tcagtgtcta ctgctggtct tggccaacag aacagaaatt 3061 tccagcaaac tccaacttat gaaaaagcac caatctgacc tgaaaaagct ggggatccta 3121 gatttcactg agcaagatgt tgcaagccac cagagctatt cccaaaagac cctgattgca 3181 ctggtcacct cgggagccct gctggctgtc ttgggcatca ctggctattt cctgatgaat 3241 cgccgcagct ggagccccac aggagaaagg ctgggcgaag acccttatta cacggaaaac 3301 ggtggaggcc agggctatag ctcaggacct gggacctccc ctgaggctca gggaaaggcc 3361 agtgtgaacc gaggggctca ggaaaacggg accggccagg ccacctccag aaacggccat 3421 tcagcaagac aacacgtggt ggctgatacc gaattgGGAT CCGACTACAA AGACCATGAC 3481 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GCGGCCGCGA 3541 AGGATCTGCG ATCGCTCCGG TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC 3601 GAGAAGTTGG GGGGAGGGGT CGGCAATTGA ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA 3661 AACTGGGAAA GTGATGTCGT GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG 3721 TATATAAGTG CAGTAGTCGC CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA 3781 CAGCTGAAGC TTCGAGGGGC TCGCATCTCT CCTTCACGCG CCCGCCGCCC TACCTGAGGC 3841 CGCCATCCAC GCCGGTTGAG TCGCGTTCTG CCGCCTCCCG CCTGTGGTGC CTCCTGAACT 3901 GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC 3961 CCTTGGAGCC TACCTAGACT CAGCCGGCTC TCCACGCTTT GCCTGACCCT GCTTGCTCAA 4021 CTCTACGTCT TTGTTTCGTT TTCTGTTCTG CGCCGTTACA GATCCAAGCT GTGACCGGCG 4081 CCTACGCTAG ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 4141 CCGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 4201 CGACCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 4261 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 4321 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 4381 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 4441 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA 4501 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 4561 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 4621 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 4681 CGGTGCCTGA GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT 4741 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA 4801 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC 4861 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC 4921 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT 4981 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG 5041 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC 5101 CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA 5161 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG 5221 GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC 5281 CCCGCCTGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT 5341 TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAAATAA GATCTGCTTT 5401 TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC 5461 TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG 5521 CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA 5581 AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA 5641 AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA 5701 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT 5761 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC 5821 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC 5881 CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 5941 AGACTTTTGC AGAGACGGCC CAAATTCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA 6001 TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 6061 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA 6121 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG 6181 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 6241 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 6301 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 6361 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 6421 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 6481 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 6541 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 6601 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 6661 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 6721 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 6781 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 6841 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 6901 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 6961 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 7021 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 7081 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 7141 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 7201 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 7261 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 7321 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 7381 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 7441 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 7501 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 7561 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 7621 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 7681 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 7741 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 7801 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 7861 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 7921 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 7981 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 8041 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 8101 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT 8161 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG 8221 TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC 8281 CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT 8341 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC 8401 GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCCAT TCGCCATTCA GGCTGCGCAA 8461 CTGTTGGGAA GGGCGATCGG TGCGGGCCTC TTCGCTATTA CGCCAGCTGG CGAAAGGGGG 8521 ATGTGCTGCA AGGCGATTAA GTTGGGTAAC GCCAGGGTTT TCCCAGTCAC GACGTTGTAA 8581 AACGACGGCC AGTGCCAAGC TG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.