pTrc99a-pyrB plasmid

pTrc99a-pyrB plasmid

PVT96347

pTrc99a-pyrB plasmid Information

Plasmid name: pTrc99a-pyrB(ecoli)
Alias: -
Gene lenght: 936bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: E.coli
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Trc
Replicon: PBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pTRC99C-F
Reverse primer:
Copy number: High
Induction:

pTrc99a-pyrB plasmid Description

pTrc99a-pyrB is a plasmid suitable for protein expression in Escherichia coli.

pTrc99a-pyrB plasmid Sequence

1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 241 taacaatttc acacaggaaa tggctaatcc gctatatcag aaacatatca tttccataaa 301 cgaccttagt cgcgatgacc ttaatctggt gctggcgaca gcggcgaaac tgaaagcaaa 361 cccgcaacca gagctgttga agcacaaagt cattgccagc tgtttcttcg aagcctctac 421 ccgtacccgc ctctctttcg aaacatctat gcaccgcctg ggggccagcg tggtgggctt 481 ctccgacagc gccaatacat cactgggtaa aaagggcgaa acgctggccg ataccatttc 541 ggttatcagc acttacgtcg atgcgatagt gatgcgtcat ccgcaggaag gtgcggcgcg 601 cctggccacc gagttttccg gcaatgtacc ggtactgaat gccggtgatg gctccaacca 661 acatccgacg caaaccttgc tggacttatt cactattcag gaaacccagg ggcgtctgga 721 caatctccac gtcgcaatgg ttggtgacct gaaatatggc cgcaccgttc actccctgac 781 tcaggcgtta gcgaagttcg acggcaaccg tttttacttc atcgcgccgg acgcgctggc 841 aatgccgcaa tacattctgg atatgctcga tgaaaaaggg atcgcatgga gtctgcacag 901 ctctattgaa gaagtgatgg cggaagtaga catcctgtac atgacccgcg tgcaaaaaga 961 gcgtctggac ccgtccgagt acgccaacgt gaaagcgcag tttgttcttc gcgccagcga 1021 tctccacaac gccaaagcca atatgaaagt gctgcatccg ctgccgcgtg ttgatgagat 1081 tgcgacggat gttgataaaa cgccacacgc ctggtacttc cagcaggcag gcaacgggat 1141 tttcgctcgc caggcgttac tggcactggt tctgaatcgc gatctggtac tgtaacggta 1201 cccggggatc ctctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag 1261 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 1321 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 1381 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1501 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 1561 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 1621 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt 1681 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 1741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 1801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 1861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 1921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 1981 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 2041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 2101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 2161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 2221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 2281 cgagcgtgac accacgatgc ctacagcaat ggcaacaacg ttgcgcaaac tattaactgg 2341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 2401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 2461 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 2521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 2581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 2641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 2701 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 2761 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 2821 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 2881 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 2941 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 3001 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 3061 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 3121 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 3181 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 3241 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 3301 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 3361 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 3421 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 3481 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 3541 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg 3601 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 3661 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 3721 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 3781 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 3841 aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg catttacgtt gacaccatcg 3901 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg 3961 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc 4021 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag 4081 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg 4141 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc 4201 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga 4261 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac 4321 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag 4381 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca 4441 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg 4501 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc 4561 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag 4621 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg 4681 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta 4741 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag 4801 acagctcatg ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc ctgctggggc 4861 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc 4921 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct 4981 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa 5041 gcgggcagtg agcgcaacgc aattaatgtg agttagcgcg aattgatctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.