pLV3-CMV-DLX6-3×FLAG-CopGFP-Pu

pLV3-CMV-DLX6-3×FLAG-CopGFP-Puro plasmid

PVT96194

pLV3-CMV-DLX6-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-DLX6(human)-3×FLAG-CopGFP-Puro
Alias: NM_005222.4
Gene lenght: 879bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-DLX6-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-DLX6-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-DLX6-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tgaccatgac tacgatggct 2341 gacggcttgg aaggccagga ctcgtccaaa tccgccttca tggagttcgg gcagcagcag 2401 cagcagcagc agcaacagca gcagcagcag cagcagcaac agcaacagcc gccgccgccg 2461 ccgccgccgc cgccgcagcc gcactcgcag cagagctccc cggccatggc aggcgcgcac 2521 taccctctgc actgcctgca ctcggcggcg gcggcggcag cggccggctc gcaccaccac 2581 caccaccacc agcaccacca ccacggctcg ccctacgcgt cgggcggagg gaactcctac 2641 aaccaccgct cgctcgccgc ctacccctac atgagccact cgcagcacag cccttacctc 2701 cagtcctacc acaacagcag cgcagccgcc cagacgcgag gggacgacac agatcaacaa 2761 aaaactacag tgattgaaaa cggggaaatc aggttcaatg gaaaagggaa aaagattcgg 2821 aagcctcgga ccatttattc cagcctgcag ctccaggctt taaaccatcg ctttcagcag 2881 acacagtatc tggcccttcc agagagagcc gaactggcag cttccttagg actgacacaa 2941 acacaggtga agatatggtt tcagaacaaa cgctctaagt ttaagaaact gctgaagcag 3001 ggcagtaatc ctcatgagag cgaccccctc cagggctcgg cggccctgtc gccacgctcg 3061 ccagcgctgc ctccagtctg ggacgtttct gcctcggcca agggtgtcag tatgcccccc 3121 aacagctaca tgcctggcta ttctcactgg tactcctctc cacaccagga cacgatgcag 3181 agaccacaga tgatgggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3241 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 3301 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3361 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 3421 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 3481 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 3541 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 3601 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 3661 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 3721 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 3781 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 3841 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 3901 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 3961 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4021 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4081 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4141 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 4201 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4261 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4321 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 4381 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 4441 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 4501 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 4561 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 4621 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 4681 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 4741 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 4801 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 4861 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 4921 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 4981 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5041 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5101 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5161 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5221 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5281 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5341 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5401 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5461 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5521 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5581 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5641 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5701 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5761 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 5821 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 5881 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 5941 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6001 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6061 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6121 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6181 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 6241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 6301 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 6361 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6481 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 6541 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 6601 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 6661 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 6721 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 6781 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 6841 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 6901 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 6961 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7021 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7081 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7141 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7201 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7261 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7321 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7381 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7441 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7501 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7561 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7621 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7681 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7741 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 7801 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 7861 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 7921 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 7981 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8041 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8101 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8161 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8221 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8281 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8341 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8401 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8461 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8521 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8581 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8641 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8701 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 8761 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 8821 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 8881 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 8941 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9001 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9061 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9121 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.