pLVX-EF1alpha-eGFP-2xStrep-IRE

pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro plasmid

PVT96175

pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro plasmid Information

Plasmid name: pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro plasmid Description

pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 3601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 3661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 3721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg 3781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 3841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 3901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 3961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag 4021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 4081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 4141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 4201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 4261 gtgcggccgc tcgagggagg cggtggatgg agccaccccc agttcgagaa gggcggcggc 4321 agcggcggcg gcagcggcgg cggcagctgg agccaccccc agttcgagaa ggggcccgtt 4381 taaggatccc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 4441 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat 4501 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 4561 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 4621 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 4681 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 4741 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 4801 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 4861 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 4921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca 4981 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 5041 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 5101 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 5161 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 5221 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 5281 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 5341 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 5401 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 5461 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 5521 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 5581 gcaagcccgg tgcctagacg cgtctggaac aatcaacctc tggattacaa aatttgtgaa 5641 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5701 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5761 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5821 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5881 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5941 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6001 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 6061 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6121 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 6181 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag 6241 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag 6301 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 6361 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 6421 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 6481 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 6541 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 6601 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 6661 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 6721 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 6781 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 6841 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6901 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 6961 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 7021 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 7081 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc 7141 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 7201 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 7261 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 7321 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 7381 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 7441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 7501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 7561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 7621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 7681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 7741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 7801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 7861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 7921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 7981 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 8041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 8101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 8161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 8221 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 8281 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 8341 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 8401 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 8461 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 8521 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 8581 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 8641 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 8701 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 8761 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 8821 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 8881 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 8941 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 9001 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 9061 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 9121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 9181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 9241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 9301 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta 9361 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 9421 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg 9481 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc 9541 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta 9601 aagaaattgt atttgttaaa tatgtactac aaacttagta gt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.