pLV3-CMV-Irf3-3×Myc-Hyg plasmi

pLV3-CMV-Irf3-3×Myc-Hyg plasmid

PVT96168

pLV3-CMV-Irf3-3×Myc-Hyg plasmid Information

Plasmid name: pLV3-CMV-Irf3(mouse)-3×Myc-Hyg
Alias: NM_016849.4;C920001K05Rik; IRF-3
Gene lenght: 1257bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Irf3-3×Myc-Hyg plasmid Description

pLV3-CMV-Irf3-3×Myc-Hyg It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Irf3-3×Myc-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agaGCCACCA TGGAAACCCC GAAACCGCGG ATTTTGCCCT GGCTGGTGTC ACAGCTGGAC 2341 CTGGGGCAGC TGGAAGGCGT GGCCTGGCTG GACGAGAGCC GAACGAGGTT CAGGATCCCG 2401 TGGAAGCATG GCCTACGGCA GGACGCACAG ATGGCTGACT TTGGCATCTT CCAGGCCTGG 2461 GCAGAAGCCA GTGGTGCCTA CACCCCGGGG AAGGATAAGC CGGACGTGTC AACCTGGAAG 2521 AGGAATTTCC GGTCAGCCCT GAACCGGAAA GAAGTGTTGC GGTTAGCTGC TGACAATAGC 2581 AAGGACCCTT ATGACCCTCA TAAAGTGTAT GAGTTTGTGA CTCCAGGGGC GCGGGACTTC 2641 GTACATCTGG GTGCCTCTCC TGACACCAAT GGCAAAAGCA GCCTGCCTCA CTCCCAGGAA 2701 AACCTACCGA AGTTATTTGA TGGCCTGATC TTGGGGCCCC TCAAAGATGA GGGGTCCTCA 2761 GATCTGGCTA TTGTTTCTGA TCCTTCTCAA CAACTGCCAA GCCCCAATGT GAACAACTTC 2821 CTAAACCCTG CACCCCAAGA AAATCCACTG AAGCAGCTGC TAGCTGAGGA ACAATGGGAG 2881 TTCGAGGTGA CCGCCTTCTA CCGAGGCCGC CAGGTCTTCC AGCAGACACT CTTTTGCCCG 2941 GGGGGCCTGC GGCTGGTGGG CAGCACAGCT GACATGACAC TGCCCTGGCA GCCAGTCACC 3001 CTGCCCGATC CTGAGGGGTT TCTGACGGAC AAGCTTGTGA AGGAGTACGT GGGGCAGGTG 3061 CTCAAAGGGC TGGGCAATGG GCTGGCACTG TGGCAGGCTG GGCAGTGCCT CTGGGCCCAG 3121 CGCCTAGGCC ACTCCCACGC CTTCTGGGCT CTGGGGGAGG AGCTGCTTCC AGACAGTGGG 3181 CGAGGGCCTG ATGGAGAGGT CCACAAGGAC AAGGACGGAG CCGTGTTCGA CCTCAGGCCC 3241 TTCGTGGCAG ATCTGATTGC CTTCATGGAA GGAAGTGGAC ACTCCCCACG CTACACTCTG 3301 TGGTTCTGCA TGGGGGAAAT GTGGCCCCAG GACCAGCCAT GGGTCAAGAG GCTTGTGATG 3361 GTCAAGGTTG TTCCTACATG TCTTAAGGAG CTGTTAGAGA TGGCCCGGGA AGGGGGAGCC 3421 TCTTCACTGA AAACCGTGGA CTTGCACATC TCCAACAGCC AGCCTATCTC CCTTACCTCT 3481 GACCAGTACA AGGCCTACCT CCAGGACTTG GTGGAGGACA TGGACTTCCA GGCCACTGGA 3541 AATATCCTCG AGGAACAAAA ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT 3601 GAAGAAGATT TAGAGCAAAA GTTAATTTCC GAGGAGGACT TATAGggatc cgcggccgca 3661 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 3721 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 3781 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 3841 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 3901 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 3961 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 4021 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 4081 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 4141 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 4201 cctacgtcga catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg 4261 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt 4321 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt 4381 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag 4441 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg 4501 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg 4561 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg 4621 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc 4681 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg 4741 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg 4801 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact 4861 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc 4921 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg 4981 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga 5041 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg 5101 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct 5161 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc 5221 cgagggcaaa ggaataggtc gacaatcaac ctctggatta caaaatttgt gaaagattga 5281 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5341 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5401 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5461 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5521 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5581 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 5641 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 5701 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 5761 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 5821 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 5881 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 5941 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6001 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 6061 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 6121 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6181 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 6241 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 6301 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 6361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 6421 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 6481 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 6541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 7141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 7381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 7501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 7561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 8101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 8161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 8221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 8281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 8701 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 8761 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 8821 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 8881 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 8941 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 9001 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 9061 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 9121 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.