pLV2-CMV-TNFRSF18-AcGFP1-Puro

pLV2-CMV-TNFRSF18-AcGFP1-Puro plasmid

PVT96156

pLV2-CMV-TNFRSF18-AcGFP1-Puro plasmid Information

Plasmid name: pLV2-CMV-TNFRSF18(human)-AcGFP1-Puro
Alias: NM_004195.3;AITR; CD357; ENERGEN; GITR; GITR-D
Gene lenght: 723bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-TNFRSF18-AcGFP1-Puro plasmid Description

pLV2-CMV-TNFRSF18-AcGFP1-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-TNFRSF18-AcGFP1-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggcaca gcacggggcg atgggcgcgt ttcgggccct 2881 gtgcggcctg gcgctgctgt gcgcgctcag cctgggtcag cgccccaccg ggggtcccgg 2941 gtgcggccct gggcgcctcc tgcttgggac gggaacggac gcgcgctgct gccgggttca 3001 cacgacgcgc tgctgccgcg attacccggg cgaggagtgc tgttccgagt gggactgcat 3061 gtgtgtccag cctgaattcc actgcggaga cccttgctgc acgacctgcc ggcaccaccc 3121 ttgtccccca ggccaggggg tacagtccca ggggaaattc agttttggct tccagtgtat 3181 cgactgtgcc tcggggacct tctccggggg ccacgaaggc cactgcaaac cttggacaga 3241 ctgcacccag ttcgggtttc tcactgtgtt ccctgggaac aagacccaca acgctgtgtg 3301 cgtcccaggg tccccgccgg cagagccgct tgggtggctg accgtcgtcc tcctggccgt 3361 ggccgcctgc gtcctcctcc tgacctcggc ccagcttgga ctgcacatct ggcagctgag 3421 gagtcagtgc atgtggcccc gagagaccca gctgctgctg gaggtgccgc cgtcgaccga 3481 agacgccaga agctgccagt tccccgagga agagcggggc gagcgatcgg cagaggagaa 3541 ggggcggctg ggagacctgt gggtggGCGG CGGAGGGAGT GGAGGAGGGG GAAGCAAgga 3601 tccaccggtc atggtgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat 3661 cgagctgaat ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga 3721 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc 3781 ctggcccacc ctggtgacca ccctgagcta cggcgtgcag tgcttctcac gctaccccga 3841 tcacatgaag cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg 3901 caccatcttc ttcgaggatg acggcaacta caagtcgcgc gccgaggtga agttcgaggg 3961 cgataccctg gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg atggcaacat 4021 cctgggcaat aagatggagt acaactacaa cgcccacaat gtgtacatca tgaccgacaa 4081 ggccaagaat ggcatcaagg tgaacttcaa gatccgccac aacatcgagg atggcagcgt 4141 gcagctggcc gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc 4201 cgataaccac tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga 4261 tcacatgatc tacttcggct tcgtgaccgc cgccgccatc acccacggca tggatgagct 4321 gtacaagtga gcggccgcga ctctagataa ttctaccggg taggggaggc gcttttccca 4381 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc 4441 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt 4501 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag 4561 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg 4621 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt 4681 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg 4741 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg 4801 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc 4861 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 4921 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 4981 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 5041 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 5101 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 5161 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 5221 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 5281 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 5341 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 5401 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 5461 gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa 5521 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 5581 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 5641 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 5701 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 5761 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 5821 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 5881 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 5941 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 6001 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 6061 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag 6121 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag 6181 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 6241 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 6301 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 6361 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 6421 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 6481 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 6541 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 6601 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 6661 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 6721 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6781 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 6841 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 6901 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6961 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac 7021 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 7081 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 7141 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 7201 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 7261 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 7321 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 7381 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 7441 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 7501 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 7561 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 7621 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 7681 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7741 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7801 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7861 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 7921 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7981 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 8041 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 8101 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 8161 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 8221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 8281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 8341 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 8401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 8461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 8521 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 8581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 8641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 8701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 8761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 8821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 8881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 8941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 9001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 9061 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 9121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 9181 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt 9241 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 9301 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact 9361 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg 9421 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt 9481 aaagaaattg tatttgttaa atatgtacta caaacttagt agt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.