pLV3-CMV-Sqstm1-3×HA-mCherry-P

pLV3-CMV-Sqstm1-3×HA-mCherry-Puro plasmid

PVT96131

pLV3-CMV-Sqstm1-3×HA-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-Sqstm1(mouse)-3×HA-mCherry-Puro
Alias: NM_011018.3;A170; OSF-6; Osi; p62; STAP; STONE14
Gene lenght: 1326bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Sqstm1-3×HA-mCherry-Puro plasmid Description

pLV3-CMV-Sqstm1-3×HA-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Sqstm1-3×HA-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcgtc gttcacggtg aaggcctatc ttctgggcaa 2341 ggaggaggcg acccgcgaga tccgccgctt cagcttctgc ttcagcccgg agccggaggc 2401 ggaagcccaa gccgcggccg gcccggggcc ctgcgagagg ctgctgagcc gagtggctgt 2461 gctgttcccc acgctgaggc ctggcggctt ccaggcgcac taccgcgatg aggatgggga 2521 cttggttgcc ttttccagtg atgaggagct gacaatggct atgtcctatg tgaaagatga 2581 catcttccgc atctacatta aagagaagaa ggagtgccgg cgggaacatc gcccaccatg 2641 tgctcaggag gcaccccgaa acatggtgca ccccaatgtg atctgtgatg gttgcaacgg 2701 gcctgtggtg ggaactcgct ataagtgcag tgtgtgccca gactacgacc tgtgcagcgt 2761 gtgcgagggg aagggcctgc acagggaaca cagcaagctc atctttccca acccctttgg 2821 ccacctctct gatagcttct ctcatagccg ctggcttcgg aagctgaaac atggacactt 2881 tggctggcct ggctgggaga tgggcccacc ggggaactgg agcccacgtc ctcctcgtgc 2941 aggggatggc cgcccttgcc ctacagctga gtcagcttct gctccaccag aagatcccaa 3001 tgtcaatttc ctgaagaatg tgggggagag tgtggcagct gccctcagcc ctctaggcat 3061 tgaggttgac attgatgtgg aacatggagg gaagagaagc cgcctgacac ccactacccc 3121 agaaagttcc agcacaggca cagaagacaa gagtaacact cagccaagca gctgctcttc 3181 ggaagtcagc aaacctgacg gggctgggga gggccctgct cagtctctga cagagcaaat 3241 gaaaaagata gccttggagt cggtgggaca gccagaggaa cagatggagt cgggaaactg 3301 ctcaggagga gacgatgact ggacacattt gtcttcaaaa gaagtggacc catctacagg 3361 tgaactccag tctctacaga tgccagaatc ggaagggcca agctctctag acccctcaca 3421 ggaaggaccc acagggctga aggaagctgc cctataccca catctcccac cagaggctga 3481 tccccggctg attgagtccc tctcccagat gctgtccatg ggtttctcgg atgaaggcgg 3541 ctggctcacc aggctcctac agaccaagaa ttacgacatc ggggctgctc tggacacgat 3601 ccagtattcg aagcaccctc caccattggg atccTACCCA TACGACGTAC CTGATTATGC 3661 AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT TATGATGTCC CAGACTACGC 3721 ATAGgcggcc gcGaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat 3781 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa 3841 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 3901 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 3961 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc 4021 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg 4081 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc 4141 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga 4201 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca 4261 agctgtgacc ggcgcctacg ctagacgcca ccatggtgag caagggcgag gaggataaca 4321 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 4381 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 4441 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 4501 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 4561 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 4621 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 4681 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 4741 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 4801 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 4861 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 4921 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 4981 gcatggacga gctgtacaag ggatctcgcg agggcagagg aagtcttcta acatgcggtg 5041 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc 5101 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 5161 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 5221 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 5281 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 5341 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 5401 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 5461 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 5521 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 5581 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 5641 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga 5701 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5761 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5821 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5881 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5941 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 6001 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 6061 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 6121 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 6181 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc 6241 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 6301 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc 6361 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 6421 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 6481 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 6541 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 6601 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 6661 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 6721 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 6781 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 6841 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 6901 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt 6961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 7381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 7441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 7501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9061 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9121 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9181 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9241 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9301 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 9361 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 9421 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 9481 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 9541 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.