pLV3-U6-Shisa4-shRNA4-CopGFP-P

pLV3-U6-Shisa4-shRNA4-CopGFP-Puro plasmid

PVT96070

pLV3-U6-Shisa4-shRNA4-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-Shisa4(mouse)-shRNA4-CopGFP-Puro
Alias: CAGAGCACGTTTGAAGGCCA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-Shisa4-shRNA4-CopGFP-Puro plasmid Description

pLV3-U6-Shisa4-shRNA4-CopGFP-Puro This is a plasmid suitable for interference silencing in mammalian cells, and it can also be used for lentivirus packaging.

pLV3-U6-Shisa4-shRNA4-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1441 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1501 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1561 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1621 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1681 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1741 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtATCGGTta 1801 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 1921 tcgatactag tGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG 1981 CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA 2041 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA 2101 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 2161 TGTGGAAAGG ACTCATCgct agcgaattcg CAGAGCACGT TTGAAGGCCA ctcgagTGGC 2221 CTTCAAACGT GCTCTGTTTT TTggatccgc ggccgcGaag gatctgcgat cgctccggtg 2281 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 2341 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 2401 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 2461 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc 2521 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc 2581 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt 2641 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca 2701 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt 2761 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg 2821 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga 2881 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga 2941 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg 3001 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc 3061 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg 3121 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca 3181 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca 3241 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg 3301 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc 3361 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc 3421 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct 3481 tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg 3541 gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg 3601 gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg cgcctcgcca 3661 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg 3721 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac 3781 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg 3841 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg 3901 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc 3961 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc 4021 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg 4081 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg 4141 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt 4201 gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt tgtgaaagat 4261 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 4321 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 4381 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 4441 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 4501 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 4561 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 4621 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 4681 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 4741 cggctctgcg gcctcttccg cgtctccgcc ttcgccctca gacgagtcgg atctcccttt 4801 ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta 4861 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag 4921 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 4981 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 5041 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 5101 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 5161 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 5221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 5281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 5341 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 5401 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 5461 tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc 5521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 5581 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 5641 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 5701 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5761 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5821 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 5881 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 5941 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6001 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6061 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6121 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6181 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6241 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6301 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6361 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6421 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6481 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6541 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6601 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6661 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6721 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6781 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6841 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 6901 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 6961 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7021 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7081 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7141 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7201 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7261 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 7321 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7381 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7441 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7501 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7561 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7621 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 7681 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 7741 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 7801 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 7861 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 7921 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 7981 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 8041 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 8101 acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.