pLV3-CMV-CAND1-3×FLAG-CopGFP-P

pLV3-CMV-CAND1-3×FLAG-CopGFP-Puro plasmid

PVT95957

pLV3-CMV-CAND1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CAND1(human)-3×FLAG-CopGFP-Puro
Alias: NM_018448.5;TIP120; TIP120A
Gene lenght: 3690bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CAND1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CAND1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-CAND1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgagcgcctc gtaccacatt 2341 tccaatttgc tggaaaaaat gacatccagc gacaaggact ttaggtttat ggctacaaat 2401 gatttgatga cggaactgca gaaagattcc atcaagttgg atgatgatag tgaaaggaaa 2461 gtagtgaaaa tgattttgaa gttattggaa gataaaaatg gagaggtaca gaatttagct 2521 gtcaaatgtc ttggtccttt agtgagtaaa gtgaaagaat accaagtaga gacaattgta 2581 gataccctct gcactaacat gctttctgat aaagaacaac ttcgagacat ttcaagtatt 2641 ggtcttaaaa cagtaattgg agaacttcct ccagcttcca gtggctctgc attagctgct 2701 aatgtatgta aaaagattac tggacgtctt acaagtgcaa tagcaaaaca ggaagatgtc 2761 tctgttcagc tagaagcctt ggatattatg gctgatatgt tgagcaggca aggaggactt 2821 cttgttaatt tccatccttc aattctgacc tgtctacttc cccagttgac cagccctaga 2881 cttgcagtga ggaaaagaac cattatcgct cttggccatc tggttatgag ctgtggaaat 2941 atagtttttg tagatcttat tgaacatctg ttgtcagagt tgtccaaaaa tgattctatg 3001 tcaacaacaa gaacctacat acaatgtatt gctgctatta gtaggcaagc tggtcataga 3061 ataggtgaat accttgagaa gataattcct ttggtggtaa aattttgcaa tgtagatgat 3121 gatgaattaa gagagtactg tattcaagcc tttgaatcat ttgtaagaag atgtcctaag 3181 gaagtatatc ctcatgtttc taccattata aatatttgtc ttaaatatct tacctatgat 3241 ccaaattata attacgatga tgaagatgaa gatgaaaatg caatggatgc tgatggtggt 3301 gatgatgatg atcaagggag tgatgatgaa tacagtgatg atgatgacat gagttggaaa 3361 gtgagacgtg cagctgcgaa gtgcttggat gctgtagtta gcacaaggca tgaaatgctt 3421 ccagaattct acaagaccgt ctctcctgca ctaatatcca gatttaaaga gcgtgaagag 3481 aatgtaaagg cagatgtttt tcacgcatac ctttctcttt tgaagcaaac tcgtcctgta 3541 caaagttggc tatgtgaccc tgatgcaatg gagcagggag aaacaccttt aacaatgctt 3601 cagagtcagg ttcccaacat tgttaaagct cttcacaaac agatgaaaga aaaaagtgtg 3661 aagacccgac agtgttgttt taacatgtta actgagctgg taaatgtatt acctggggcc 3721 ctaactcaac acattcctgt acttgtacca ggaatcattt tctcactgaa tgataaatca 3781 agctcatcga atttgaagat cgatgctttg tcatgtctat acgtaatcct ctgtaaccat 3841 tctcctcaag tcttccatcc tcacgttcag gctttggttc ctccagtggt ggcttgtgtt 3901 ggagacccat tttacaaaat tacatctgaa gcacttcttg ttactcaaca gcttgtcaaa 3961 gtaattcgtc ctttagatca gccttcctcg tttgatgcaa ctccttatat caaagatcta 4021 tttacctgta ccattaagag attaaaagca gctgacattg atcaggaagt caaggaaagg 4081 gctatttcct gtatgggaca aattatttgc aaccttggag acaatttggg ttctgacttg 4141 cctaatacac ttcagatttt cttggagaga ctaaagaatg aaattaccag gttaactaca 4201 gtaaaggcat tgacactgat tgctgggtca cctttgaaga tagatttgag gcctgttctg 4261 ggagaagggg ttcctatcct tgcttcattt cttagaaaaa accagagagc tttgaaactg 4321 ggtactcttt ctgcccttga tattctaata aaaaactata gtgacagctt gacagctgcc 4381 atgattgatg cagttctaga tgagctccca cctcttatca gcgaaagtga tatgcatgtt 4441 tcacaaatgg ccatcagttt tcttaccact ttggcaaaag tatatccctc ctccctttca 4501 aagataagtg gatccattct caatgaactt attggacttg tgagatcacc cttattgcag 4561 gggggagctc ttagtgccat gctagacttt ttccaagctc tggttgtcac tggaacaaat 4621 aatttaggat acatggattt gttgcgcatg ctgactggtc cagtttactc tcagagcaca 4681 gctcttactc ataagcagtc ttattattcc attgccaaat gtgtagctgc ccttactcga 4741 gcatgcccta aagagggacc agctgtagta ggtcagttta ttcaagatgt caagaactca 4801 aggtctacag attccattcg tctcttagct ctactttctc ttggagaagt tgggcatcat 4861 attgacttaa gtggacagtt ggaactaaaa tctgtaatac tagaagcttt ctcatctcct 4921 agtgaagaag tcaaatcagc tgcatcctat gcattaggca gcattagtgt gggcaacctt 4981 cctgaatatc tgccgtttgt cctgcaagaa ataactagtc aacccaaaag gcagtatctt 5041 ttacttcatt ccttgaagga aattattagc tctgcatcag tggtgggcct taaaccatat 5101 gttgaaaaca tctgggcctt attactaaag cactgtgagt gtgcagagga aggaaccaga 5161 aatgttgttg ctgaatgtct aggaaaactc actctaattg atccagaaac tctccttcca 5221 cggcttaagg ggtacttgat atcaggctca tcatatgccc gaagctcagt ggttacggct 5281 gtgaaattta caatttctga ccatccacaa cctattgatc cactgttaaa gaactgcata 5341 ggtgatttcc taaaaacttt ggaagaccca gatttgaatg tgagaagagt agccttggtc 5401 acatttaatt cagcagcaca taacaagcca tcattaataa gggatctatt ggatactgtt 5461 cttccacatc tttacaatga aacaaaagtt agaaaggagc ttataagaga ggtagaaatg 5521 ggtccattta aacatacggt tgatgatggt ctggatatta gaaaggcagc atttgagtgt 5581 atgtacacac ttctagacag ttgtcttgat agacttgata tctttgaatt tctaaatcat 5641 gttgaagatg gtttgaagga ccattatgat attaagatgc tgacattttt aatgttggtg 5701 agactgtcta ccctttgtcc aagtgcagta ctgcagaggt tggaccgact tgttgagcca 5761 ttacgtgcaa catgtacaac taaggtaaag gcaaactcag taaagcagga gtttgaaaaa 5821 caagatgaat taaagcgatc tgccatgaga gcagtagcag cactgctaac cattccagaa 5881 gcagagaaga gtccactgat gagtgaattc cagtcacaga tcagttctaa ccctgagctg 5941 gcggctatct ttgaaagtat ccagaaagat tcatcatcta ctaacttgga atcaatggac 6001 actagtggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 6061 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 6121 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 6181 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 6241 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 6301 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 6361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 6421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 6481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 6541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 6601 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 6661 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 6721 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 6781 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 6841 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 6901 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 6961 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 7021 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 7081 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 7141 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 7201 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 7261 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 7321 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 7381 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 7441 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 7501 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 7561 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 7621 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 7681 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 7741 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 7801 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 7861 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 7921 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 7981 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 8041 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 8101 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 8161 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 8221 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 8281 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 8341 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 8401 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 8461 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 8521 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 8581 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 8641 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 8701 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 8761 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 8821 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 8881 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 8941 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 9001 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 9061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 9121 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 9181 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 9241 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 9301 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 9361 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 9421 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 9481 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 9541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 9601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 9661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 9721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 9781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 9841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 9901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 9961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 10021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 10081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 10141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 10201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 10261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 10321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 10381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 10441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 10501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 10561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 10621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 10681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 10741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 10801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 10861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 10921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 10981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 11041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 11101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 11161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 11221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 11281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 11341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 11401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 11461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 11521 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 11581 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 11641 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 11701 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 11761 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 11821 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 11881 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 11941 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.