pCMV-ZDHHC18-3×FLAG-mCMV-EGFP

pCMV-ZDHHC18-3×FLAG-mCMV-EGFP plasmid

PVT95932

pCMV-ZDHHC18-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-ZDHHC18(human)-3×FLAG-mCMV-EGFP
Alias: NM_032283.3;DHHC-18; DHHC18;
Gene lenght: 1164bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-ZDHHC18-3×FLAG-mCMV-EGFP plasmid Description

pCMV-ZDHHC18-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-ZDHHC18-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCATGAAG GACTGCGAGT ACCAGCAGAT 661 CAGCCCCGGG GCCGCCCCGC TGCCCGCCTC CCCGGGGGCG CGCCGTCCCG GCCCCGCCGC 721 GTCCCCGACT CCGGGCCCCG GGCCCGCGCC GCCCGCCGCC CCCGCCCCGC CGCGCTGGAG 781 CAGCAGCGGC AGCGGCAGCG GCAGCGGGAG CGGGAGCCTC GGCCGCCGCC CACGGCGCAA 841 GTGGGAGGTG TTCCCGGGTC GCAATCGCTT CTACTGCGGC GGCCGCCTCA TGCTGGCCGG 901 CCACGGCGGC GTCTTCGCGC TCACGCTGCT GCTCATCCTC ACCACCACCG GCCTCTTCTT 961 CGTCTTTGAC TGTCCCTACC TGGCTCGCAA GCTGACCCTT GCCATCCCCA TCATCGCTGC 1021 CATCCTCTTC TTCTTCGTCA TGAGCTGCCT GCTGCAGACA AGCTTCACCG ACCCTGGGAT 1081 CCTGCCCCGG GCCACTGTCT GTGAAGCAGC CGCCCTGGAG AAACAGATCG ACAACACAGG 1141 CAGTTCTACA TACCGGCCAC CCCCTCGGAC CCGGGAGGTG CTGATCAACG GGCAGATGGT 1201 GAAGCTGAAG TACTGCTTCA CCTGCAAGAT GTTCCGGCCA CCCCGAACCT CACACTGCAG 1261 TGTCTGCGAC AACTGTGTGG AACGATTTGA CCATCACTGC CCCTGGGTGG GCAACTGTGT 1321 GGGGAGACGG AACTATCGCT TCTTCTACGC GTTTATTCTC TCCCTCTCAT TCCTGACGGC 1381 CTTCATCTTC GCCTGTGTGG TCACCCACCT GACGTTGCGC GCTCAGGGAA GCAACTTCCT 1441 CTCCACTCTG AAGGAGACAC CAGCAAGCGT GCTGGAGTTG GTGATCTGCT TCTTCTCCAT 1501 CTGGTCCATT CTGGGCCTCT CAGGGTTTCA CACGTACCTC GTCGCCTCCA ACCTGACTAC 1561 TAATGAAGAC ATCAAAGGCT CGTGGTCCAG CAAGAGGGGC GGTGAGGCCT CTGTCAACCC 1621 CTACAGCCAT AAAAGTATTA TCACCAACTG CTGTGCTGTG CTCTGTGGCC CCCTACCTCC 1681 CAGCCTAATT GACCGGAGGG GATTTGTGCA GTCCGACACC GTGTTGCCCT CACCCATCAG 1741 AAGCGATGAG CCAGCCTGCA GAGCCAAGCC TGATGCCAGC ATGGTAGGAG GCCACCCCAA 1801 GCTTACGCGT GACTACAAAG ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA 1861 GGATGACGAT GACAAGTGAG GATCCGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT 1921 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 1981 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 2041 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 2101 AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GTCTAGAGAT ATACTGAGTC 2161 ATTAGGGACT TTCCAATGGG TTTTGCCCAG TACATAAGGT CAATAGGGGT GAATCAACAG 2221 GAAAGTCCCA TTGGAGCCAA GTACACTGAG TCAATAGGGA CTTTCCATTG GGTTTTGCCC 2281 AGTACAAAAG GTCAATAGGG GGTGAGTCAA TGGGTTTTTC CCATTATTGG CACGTACATA 2341 AGGTCAATAG GGGTGAGTCA TTGGGTTTTT CCAGCCAaTT TAATTAAAAC GCCATGTACT 2401 TTCCCACCAT TGACGTCAAT GGGCTATTGA AACTAATGCA ACGTGACCTT TAAACGGTAC 2461 TTTCCCATAG CTGATTAATG GGAAAGTACC GTTCTCGAGC CAATACACGT CAATGGGAAG 2521 TGAAAGGGCA GCCAAAACGT AACACCGCCC CGGTTTTCCC CTGGAAATTC CATATTGGCA 2581 CGCATTCTAT TGGCTGAGCT GCGTTCTACG TGGGTATAAG AGGCGCGACC AGCGTCGGTA 2641 CCGTCGCAGT CTTCGGTCTG ACCACCGTAG AACGCAGATC accggtcgcc accatggtga 2701 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 2761 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 2821 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 2881 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 2941 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 3001 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 3061 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 3121 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 3181 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 3241 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 3301 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 3361 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc 3421 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 3481 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 3541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 3601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg 3661 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 3721 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 3781 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 3841 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 3901 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 3961 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 4021 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 4081 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 4141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 4201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 4261 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 4321 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 4381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 4441 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 4501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 4561 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 4621 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 4681 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 4741 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 4801 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 4861 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 4921 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 4981 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 5041 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 5101 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 5161 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 5221 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 5281 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 5341 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 5401 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 5461 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 5521 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 5581 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 5641 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 5701 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 5761 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 5821 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 5881 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 5941 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 6001 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 6061 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 6121 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 6181 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 6241 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 6301 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 6361 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 6421 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 6481 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 6541 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 6601 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 6661 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 6721 tgtggataac cgtattaccg ccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.