pCMV-AKR1B1-3×FLAG-mCMV-EGFP p

pCMV-AKR1B1-3×FLAG-mCMV-EGFP plasmid

PVT95800

pCMV-AKR1B1-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-AKR1B1(human)-3×FLAG-mCMV-EGFP
Alias: NM_001628.4;ADR; ALDR1; ALR2; AR
Gene lenght: 948bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-AKR1B1-3×FLAG-mCMV-EGFP plasmid Description

pCMV-AKR1B1-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-AKR1B1-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatggca agccgtctcc tgctcaacaa 661 cggcgccaag atgcccatcc tggggttggg tacctggaag tcccctccag ggcaggtgac 721 tgaggccgtg aaggtggcca ttgacgtcgg gtaccgccac atcgactgtg cccatgtgta 781 ccagaatgag aatgaggtgg gggtggccat tcaggagaag ctcagggagc aggtggtgaa 841 gcgtgaggag ctcttcatcg tcagcaagct gtggtgcacg taccatgaga agggcctggt 901 gaaaggagcc tgccagaaga cactcagcga cctgaagctg gactacctgg acctctacct 961 tattcactgg ccgactggct ttaagcctgg gaaggaattt ttcccattgg atgagtcggg 1021 caatgtggtt cccagtgaca ccaacattct ggacacgtgg gcggccatgg aagagctggt 1081 ggatgaaggg ctggtgaaag ctattggcat ctccaacttc aaccatctcc aggtggagat 1141 gatcttaaac aaacctggct tgaagtataa gcctgcagtt aaccagattg agtgccaccc 1201 atatctcact caggagaagt taatccagta ctgccagtcc aaaggcatcg tggtgaccgc 1261 ctacagcccc ctcggctctc ctgacaggcc ctgggccaag cccgaggacc cttctctcct 1321 ggaggatccc aggatcaagg cgatcgcagc caagcacaat aaaactacag cccaggtcct 1381 gatccggttc cccatgcaga ggaacttggt ggtgatcccc aagtctgtga caccagaacg 1441 cattgctgag aactttaagg tctttgactt tgaactgagc agccaggata tgaccacctt 1501 actcagctac aacaggaact ggagggtctg tgccttgttg agctgtacct cccacaagga 1561 ttaccccttc catgaagagt ttAAGCTTAC GCGTGACTAC AAAGACCATG ACGGTGATTA 1621 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TGAGGATCCG GGCCCGTTTA 1681 AACCCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC 1741 CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA 1801 GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA 1861 GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC 1921 TATGGTCTAG AGATATACTG AGTCATTAGG GACTTTCCAA TGGGTTTTGC CCAGTACATA 1981 AGGTCAATAG GGGTGAATCA ACAGGAAAGT CCCATTGGAG CCAAGTACAC TGAGTCAATA 2041 GGGACTTTCC ATTGGGTTTT GCCCAGTACA AAAGGTCAAT AGGGGGTGAG TCAATGGGTT 2101 TTTCCCATTA TTGGCACGTA CATAAGGTCA ATAGGGGTGA GTCATTGGGT TTTTCCAGCC 2161 AaTTTAATTA AAACGCCATG TACTTTCCCA CCATTGACGT CAATGGGCTA TTGAAACTAA 2221 TGCAACGTGA CCTTTAAACG GTACTTTCCC ATAGCTGATT AATGGGAAAG TACCGTTCTC 2281 GAGCCAATAC ACGTCAATGG GAAGTGAAAG GGCAGCCAAA ACGTAACACC GCCCCGGTTT 2341 TCCCCTGGAA ATTCCATATT GGCACGCATT CTATTGGCTG AGCTGCGTTC TACGTGGGTA 2401 TAAGAGGCGC GACCAGCGTC GGTACCGTCG CAGTCTTCGG TCTGACCACC GTAGAACGCA 2461 GATCaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 2521 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 2581 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc 2641 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct 2701 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 2761 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 2821 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 2881 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg 2941 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg 3001 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 3061 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga 3121 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg 3181 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt 3241 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 3301 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 3361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 3421 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg 3481 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 3541 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 3601 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 3661 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 3721 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 3781 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 3841 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 3901 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 3961 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 4021 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg 4081 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 4141 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 4201 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 4261 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 4321 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 4381 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag 4441 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 4501 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 4561 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag 4621 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 4681 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 4741 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 4801 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 4861 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 4921 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg 4981 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 5041 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 5101 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 5161 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 5221 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 5281 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 5341 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg 5401 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 5461 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc 5521 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt 5581 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa 5641 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt 5701 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 5761 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 5821 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 5881 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 5941 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 6001 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 6061 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 6121 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 6181 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 6241 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 6301 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 6361 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 6421 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 6481 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.