pLV3-U6-KRT7-shRNA2-TagBFP-Pur

pLV3-U6-KRT7-shRNA2-TagBFP-Puro plasmid

PVT95790

pLV3-U6-KRT7-shRNA2-TagBFP-Puro plasmid Information

Plasmid name: pLV3-U6-KRT7(human)-shRNA2-TagBFP-Puro
Alias: CCCGGAATGAGATTTCAGAGA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Blue
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-KRT7-shRNA2-TagBFP-Puro plasmid Description

pLV3-U6-KRT7-shRNA2-TagBFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-KRT7-shRNA2-TagBFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtGAGGGCC TATTTCCCAT GATTCCTTCA TATTTGCATA TACGATACAA 1981 GGCTGTTAGA GAGATAATTA GAATTAATTT GACTGTAAAC ACAAAGATAT TAGTACAAAA 2041 TACGTGACGT AGAAAGTAAT AATTTCTTGG GTAGTTTGCA GTTTTAAAAT TATGTTTTAA 2101 AATGGACTAT CATATGCTTA CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2161 CTTGTGGAAA GGACTCATCg ctagcgaatt cgCCCGGAAT GAGATTTCAG AGAttcaaga 2221 gaTCTCTGAA ATCTCATTCC GGGttttttg gatccgcggc cgcGaaggat ctgcgatcgc 2281 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 2341 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 2401 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 2461 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 2521 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 2581 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 2641 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 2701 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 2761 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 2821 accgccaccA TGAGCGAGCT GATTAAGGAG AACATGCACA TGAAGCTGTA CATGGAGGGC 2881 ACCGTGGACA ACCATCACTT CAAGTGCACA TCCGAGGGCG AAGGCAAGCC CTACGAGGGC 2941 ACCCAGACCA TGAGAATCAA GGTGGTCGAG GGCGGCCCTC TCCCCTTCGC CTTCGACATC 3001 CTGGCTACTA GCTTCCTCTA CGGCAGCAAG ACCTTCATCA ACCACACCCA GGGCATCCCC 3061 GACTTCTTCA AGCAGTCCTT CCCTGAGGGC TTCACATGGG AGAGAGTCAC CACATACGAA 3121 GACGGGGGCG TGCTGACCGC TACCCAGGAC ACCAGCCTCC AGGACGGCTG CCTCATCTAC 3181 AACGTCAAGA TCAGAGGGGT GAACTTCACA TCCAACGGCC CTGTGATGCA GAAGAAAACA 3241 CTCGGCTGGG AGGCCTTCAC CGAGACGCTG TACCCCGCTG ACGGCGGCCT GGAAGGCAGA 3301 AACGACATGG CCCTGAAGCT CGTGGGCGGG AGCCATCTGA TCGCAAACAT CAAGACCACA 3361 TATAGATCCA AGAAACCCGC TAAGAACCTC AAGATGCCTG GCGTCTACTA TGTGGACTAC 3421 AGACTGGAAA GAATCAAGGA GGCCAACAAC GAGACCTACG TCGAGCAGCA CGAGGTGGCA 3481 GTGGCCAGAT ACTGCGACCT CCCTAGCAAA CTGGGGCACA AGCTTAATag atcccgcgct 3541 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 3601 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 3661 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 3721 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 3781 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 3841 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 3901 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 3961 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 4021 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 4081 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 4141 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 4201 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 4261 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 4321 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 4381 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 4441 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 4501 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 4561 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 4621 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 4681 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 4741 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 4801 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 4861 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 4921 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 4981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 5041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 5101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 5161 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 5221 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 5281 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 5341 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 5401 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 5461 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 5521 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 5581 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 5641 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 5701 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 5761 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 5821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 5881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 5941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6361 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6601 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 6661 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 6721 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 6781 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 6841 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 6901 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 6961 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 7021 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 7081 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 7141 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 7201 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 7261 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 7321 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 7381 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 7441 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 7501 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 7561 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 7621 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 7681 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 7741 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 7801 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 7861 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 7921 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 7981 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 8041 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 8101 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 8161 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.