pLV3-CMV-Slc5a3-3×HA-CopGFP-Pu

pLV3-CMV-Slc5a3-3×HA-CopGFP-Puro plasmid

PVT95696

pLV3-CMV-Slc5a3-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Slc5a3(mouse)-3×HA-CopGFP-Puro
Alias: NM_017391.3;
Gene lenght: 2154bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Slc5a3-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-Slc5a3-3×HA-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Slc5a3-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga gggctgtact ggaggcagca 2341 gacattgccg tagtggccct gtattttatc ctggtcatgt gcattggctt ttttgccatg 2401 tggaaatcta atagaagcac tgtgagtgga tacttcctgg ccgggcgctc tatgacctgg 2461 gtggcgattg gtgcctctct gtttgtgagc aatattggga gtgaacactt cattgggctg 2521 gcaggatctg gagcagcaag tggatttgca gtgggcgcat gggaattcaa tgccttactg 2581 ctcttgcaac ttctgggatg ggttttcatc cccatttaca tccggtcagg ggtatatacc 2641 atgcctgaat acttgtccaa gaggttcggt ggccatagga ttcaggtcta ttttgcagcc 2701 ttgtctctgc ttctctatat cttcaccaag ctctcagtgg atctgtattc aggggccctc 2761 tttatccagg agtccttggg ttggaacctt tatgtgtctg tcatcctgct cattggcatg 2821 actgctctgc tgactgtcac cggaggcctt gttgcagtga tctacacaga cactctgcag 2881 gccctgctca tgatcattgg ggcgctcaca cttatggtta ttagcatggt gaagattgga 2941 gggtttgagg aagttaagag aaggtacatg ttggcctcac ctgatgttgc ttccattttg 3001 ttgaagtaca acctttccaa cacaaatgct tgtatggtcc atcctaaggc gaatgcccta 3061 aaaatgttga gagacccaac agatgaagat gttccatggc ctggattcat tcttgggcaa 3121 accccagcct cggtatggta ctggtgtgct gaccaagtca tcgtacagag ggttctagca 3181 gccaaaaaca ttgctcatgc caaaggctcc actctaatgg ctggcttctt gaagcttcta 3241 ccaatgttta tcatagttgt accaggaatg atttccagga tagtgtttgc tgatgagata 3301 gcttgcatca acccagaaca ctgcatgcaa gtgtgtggaa gcagagctgg ctgctccaat 3361 attgcttacc cacgcctggt gatgaccttg gttcctgtgg gccttcgggg cctgatgatg 3421 gcagtgatga ttgcagctct gatgagtgac ttggactcca tctttaacag cgccagtacc 3481 atattcaccc tcgatgtgta caaacttatc cgcaagagtg caagctcccg ggaactaatg 3541 attgtgggca ggatatttgt ggcttttatg gttgtcatca gcatagcatg ggtgccaatt 3601 attgtggaga tgcaaggagg ccaaatgtac ctttacatcc aggaggtggc agattatctg 3661 actcctcccg tggcagccct cttccttctg gcaattttct ggaaacgctg caatgagcaa 3721 ggggcttttt atggtggaat ggcaggcttt gttcttggag ctgtccgttt gatactggct 3781 tttacctacc gtgctcctga gtgtgatcaa cctgataaca ggccaggctt catcaaagac 3841 atccattata tgtacgtggc tacggcatta ttttggatca caggactcat tactgtgatt 3901 gttagtcttc tcacaccacc tcccacaaag gatcagattc gtaccactac cttttggtca 3961 aagaagaccc tggtgacaaa ggagagctgt tctcagaaag acgaaccata caaaatgcaa 4021 gagaagagca tcctgcagtg cagcgagaat agcgaagtca tcagccatac tattcccaac 4081 gggaagtctg aagacagcat taagggactg caacctgaag acgttaatct gctggtgaca 4141 tgccgagaag agggcaaccc agtggcttcc atgggtcatt cagaggcaga aacaccagta 4201 gatgcttatt ccaatgggca agcagctctc atgggtgaga gagaaagaga gaaggaaacg 4261 gaaaatagaa gccggtattg gaagttcata gactggtttt gtggctttaa gagtaaaagc 4321 cttagcaaga ggagcctcag agacttgatg gatgaggagg ctgtgtgttt acaaatgtta 4381 gaagagactc cacaagttaa agtgatccta aacattggac tttttgctgt gtgttcactt 4441 ggaattttca tgtttgttta tttctcctta CTCGAGTACC CATACGACGT ACCTGATTAT 4501 GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT CCCAGACTAC 4561 GCATAGggat ccCTGCGATA TCCAGCgcgg ccgcGaagga tctgcgatcg ctccggtgcc 4621 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc 4681 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 4741 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 4801 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc 4861 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc 4921 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta 4981 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc 5041 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct 5101 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag 5161 agcgacgaga gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac 5221 ggcgtggagt tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc 5281 aacaagatga agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg 5341 atgggctacg gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg 5401 cacgccatca acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc 5461 gtgctgcacg tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag 5521 gtggtgggca ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc 5581 aacgccaccg tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc 5641 cgcaccttca gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac 5701 ttcaagagcg ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc 5761 cgcgtggagg agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc 5821 aagaccccca tcgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc 5881 accgccggac ccggctccac cggatctcgc gagggcagag gaagtcttct aacatgcggt 5941 gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg cctcgccacc 6001 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 6061 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 6121 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 6181 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 6241 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 6301 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 6361 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 6421 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 6481 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 6541 atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg tgaaagattg 6601 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 6661 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 6721 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 6781 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 6841 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 6901 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 6961 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 7021 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 7081 gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat ctccctttgg 7141 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 7201 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat 7261 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 7321 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 7381 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 7441 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 7501 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 7561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 7621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 7681 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 7741 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 7801 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 7861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 7921 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 7981 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 8041 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 8101 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 8161 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 8221 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 8281 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 8341 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 8401 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 8461 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 8521 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 8581 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 8641 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 8701 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 8761 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8821 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8881 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8941 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 9001 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 9061 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 9121 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 9181 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 9241 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 9301 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 9361 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 9421 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 9481 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 9541 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 9601 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 9661 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 9721 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 9781 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 9841 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 9901 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 9961 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 10021 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 10081 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 10141 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 10201 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 10261 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 10321 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 10381 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 10441 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.