pLV3-U6-SEMA7A-shRNA2-CopGFP-H

pLV3-U6-SEMA7A-shRNA2-CopGFP-Hyg plasmid

PVT95530

pLV3-U6-SEMA7A-shRNA2-CopGFP-Hyg plasmid Information

Plasmid name: pLV3-U6-SEMA7A(human)-shRNA2-CopGFP-Hyg
Alias: GGTCAGTGCTGCAATCCATTA
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-SEMA7A-shRNA2-CopGFP-Hyg plasmid Description

pLV3-U6-SEMA7A-shRNA2-CopGFP-Hyg is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-SEMA7A-shRNA2-CopGFP-Hyg plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT ACGATACAAG 1981 GCTGTTAGAG AGATAATTAG AATTAATTTG ACTGTAAACA CAAAGATATT AGTACAAAAT 2041 ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT ATGTTTTAAA 2101 ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2161 TTGTGGAAAG GACTCATCgc tagcgGGTCA GTGCTGCAAT CCATTATTCA AGAGATAATG 2221 GATTGCAGCA CTGACCtttt ttggatccgc ggccgcGaag gatctgcgat cgctccggtg 2281 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 2341 gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 2401 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 2461 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc 2521 gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc 2581 gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt 2641 taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca 2701 gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt 2761 ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac gccaccatgg 2821 agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc ggcaccctga 2881 acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag ggccgcatga 2941 ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg ctgagccacg 3001 tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag aaccccttcc 3061 tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac gaggacggcg 3121 gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc ggcgacttca 3181 aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag atcatccgca 3241 gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg 3301 cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac agccacatgc 3361 acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg ttcgccttcc 3421 gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac cagcacgcct 3481 tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct gccgtggacg 3541 gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt ctaacatgcg 3601 gtgacgtgga ggagaatccc ggccctatga aaaagcctga actcaccgcg acgtctgtcg 3661 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg 3721 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata 3781 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc 3841 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct 3901 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc 3961 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg 4021 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat 4081 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg 4141 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc 4201 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa 4261 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca 4321 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga 4381 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg 4441 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc 4501 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca 4561 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac 4621 gccccagcac tcgtccgagg gcaaaggaat aggtcgacaa tcaacctctg gattacaaaa 4681 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 4741 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 4801 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 4861 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 4921 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 4981 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 5041 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 5101 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 5161 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 5221 ggatctccct ttgggccgcc tccccgcggt acctttaaga ccaatgactt acaaggcagc 5281 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 5341 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 5401 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 5461 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 5521 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 5581 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 5641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 5701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 5761 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 5821 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 5881 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca 5941 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 6001 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 6061 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 6121 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 6181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 6241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 6301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 6361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 6421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 6481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 6541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 6601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 6661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 6721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 6781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 6841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 6901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 6961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 7021 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 7081 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 7141 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 7201 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 7261 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 7321 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 7381 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 7441 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 7501 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 7561 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 7621 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 7681 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 7741 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 7801 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 7861 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 7921 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 7981 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 8041 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 8101 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc 8161 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 8221 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 8281 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac 8341 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 8401 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 8461 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 8521 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.