pLV2-EF1a-Sod2-3×HA-MS2-4X-Pur

pLV2-EF1a-Sod2-3×HA-MS2-4X-Puro plasmid

PVT95408

pLV2-EF1a-Sod2-3×HA-MS2-4X-Puro plasmid Information

Plasmid name: pLV2-EF1a-Sod2(mouse)-3×HA-MS2-4X-Puro
Alias: NM_013671.3;MnSOD; Sod-2
Gene lenght: 666bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pLV2-EF1a-Sod2-3×HA-MS2-4X-Puro plasmid Description

pLV2-EF1a-Sod2-3×HA-MS2-4X-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-EF1a-Sod2-3×HA-MS2-4X-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaATTAGCTT GGTACgaatt 3541 cGCCACCatg ttgtgtcggg cggcgtgcag cacgggcagg aggctgggcc ctgtggccgg 3601 tgccgcgggc tcccggcaca agcacagcct cccagacctg ccttacgact atggcgcgct 3661 ggagccacac attaacgcgc agatcatgca gctgcaccac agcaagcacc acgcggccta 3721 cgtgaacaat ctcaacgcca ccgaggagaa gtaccacgag gctctggcca agggagatgt 3781 tacaactcag gtcgctcttc agcctgcact gaagttcaat ggtgggggac atattaatca 3841 caccattttc tggacaaacc tgagccctaa gggtggtgga gaacccaaag gagagttgct 3901 ggaggctatc aagcgtgact ttgggtcttt tgagaagttt aaggagaagc tgacagccgt 3961 gtctgtggga gtccaaggtt caggctgggg ctggcttggc ttcaataagg agcaaggtcg 4021 cttacagatt gctgcctgct ctaatcagga cccattgcaa ggaacaacag gccttattcc 4081 gctgctgggg attgacgtgt gggagcacgc ttactacctt cagtataaaa acgtcagacc 4141 tgactatctg aaagctattt ggaatgtaat caactgggag aatgttactg aaagatacac 4201 agcttgcaag aagTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT ATGATGTACC 4261 TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA TAGgcggccg cgcagtattc 4321 ccgggttcat tagatcctaa ggtacctaat tgcctagaaa acatgaggat cacccatgtc 4381 tgcaggtcga ctctagaaaa catgaggatc acccatgtct gcagtattcc cgggttcatt 4441 agatcctaag gtacctaatt gcctagaaaa catgaggatc acccatgtct gcaggtcgac 4501 tctagaaaac atgaggatca cccatgtgtg caccttaagc ttggcccgcg gggatccaga 4561 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg 4621 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa 4681 acaagttcgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc 4741 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac 4801 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc 4861 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga 4921 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt 4981 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct 5041 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg 5101 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt 5161 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac 5221 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5281 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5341 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5401 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 5461 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 5521 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 5581 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 5641 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 5701 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 5761 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgtctggaa 5821 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5881 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5941 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6001 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6061 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6121 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6181 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 6241 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6301 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6361 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 6421 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 6481 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 6541 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 6601 aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 6661 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca 6721 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat 6781 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat 6841 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt 6901 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc 6961 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag 7021 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg 7081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 7141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 7201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc 7261 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag 7321 gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt aatcatggtc 7381 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 7441 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 7501 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 7561 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 7621 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 7681 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 7741 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 7801 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 7861 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 7921 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 7981 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 8041 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 8101 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 8161 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 8221 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 8281 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 8341 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 8401 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 8461 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 8521 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 8581 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 8641 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 8701 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 8761 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 8821 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 8881 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 8941 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 9001 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 9061 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 9121 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 9181 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 9241 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 9301 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 9361 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 9421 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 9481 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg 9541 atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 9601 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 9661 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca 9721 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa 9781 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta 9841 caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.