pLV3-CMV-LMNB1-3×HA-EF1a-mCher

pLV3-CMV-LMNB1-3×HA-EF1a-mCherry-Puro plasmid

PVT95397

pLV3-CMV-LMNB1-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-LMNB1(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_005573.4;ADLD; ADLDAT; LMN; LMN2; LMNB; MCPH26
Gene lenght: 1758bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LMNB1-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-LMNB1-3×HA-EF1a-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-LMNB1-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggcgac tgcgaccccc gtgccgccgc ggatgggcag 2341 ccgcgctggc ggccccacca cgccgctgag ccccacgcgc ctgtcgcggc tccaggagaa 2401 ggaggagctg cgcgagctca atgaccggct ggcggtgtac atcgacaagg tgcgcagcct 2461 ggagacggag aacagcgcgc tgcagctgca ggtgacggag cgcgaggagg tgcgcggccg 2521 tgagctcacc ggcctcaagg cgctctacga gaccgagctg gccgacgcgc gacgcgcgct 2581 cgacgacacg gcccgcgagc gcgccaagct gcagatcgag ctgggcaagt gcaaggcgga 2641 acacgaccag ctgctcctca actatgctaa gaaggaatct gatcttaatg gcgcccagat 2701 caagcttcga gaatatgaag cagcactgaa ttcgaaagat gcagctcttg ctactgcact 2761 tggtgacaaa aaaagtttag agggagattt ggaggatctg aaggatcaga ttgcccagtt 2821 ggaagcctcc ttagctgcag ccaaaaaaca gttagcagat gaaactttac ttaaagtaga 2881 tttggagaat cgttgtcaga gccttactga ggacttggag tttcgcaaaa gcatgtatga 2941 agaggagatt aacgagacca gaaggaagca tgaaacgcgc ttggtagagg tggattctgg 3001 gcgtcaaatt gagtatgagt acaagctggc gcaagccctt catgagatga gagagcaaca 3061 tgatgcccaa gtgaggctgt ataaggagga gctggagcag acttaccatg ccaaacttga 3121 gaatgccaga ctgtcatcag agatgaatac ttctactgtc aacagtgcca gggaagaact 3181 gatggaaagc cgcatgagaa ttgagagcct ttcatcccag ctttctaatc tacagaaaga 3241 gtctagagca tgtttggaaa ggattcaaga attagaggac ttgcttgcta aagaaaaaga 3301 caactctcgt cgcatgctga cagacaaaga gagagagatg gcggaaataa gggatcaaat 3361 gcagcaacag ctgaatgact atgaacagct tcttgatgta aagttagccc tggacatgga 3421 aatcagtgct tacaggaaac tcttagaagg cgaagaagag aggttgaagc tgtctccaag 3481 cccttcttcc cgtgtgacag tatcccgagc atcctcaagt cgtagtgtac gtacaactag 3541 aggaaagcgg aagagggttg atgtggaaga atcagaggcg agtagtagtg ttagcatctc 3601 tcattccgcc tcagccactg gaaatgtttg catcgaagaa attgatgttg atgggaaatt 3661 tatccgcttg aagaacactt ctgaacagga tcaaccaatg ggaggctggg agatgatcag 3721 aaaaattgga gacacatcag tcagttataa atatacctca agatatgtgc tgaaggcagg 3781 ccagactgtt acaatttggg ctgcaaacgc tggtgtcaca gccagccccc caactgacct 3841 catctggaag aaccagaact cgtggggcac tggcgaagat gtgaaggtta tattgaaaaa 3901 ttctcaggga gaggaggttg ctcaaagaag tacagtcttt aaaacaacca tacctgaaga 3961 agaggaggag gaggaagaag cagctggagt ggttgttgag gaagaacttt tccaccagca 4021 gggaacccca agagcatcca atagaagctg tgcaattatg ggatccTACC CATACGACGT 4081 ACCTGATTAT GCAACTTATC CTTATGATGT ACCTGATTAT GCTAGCTATC CTTATGATGT 4141 CCCAGACTAC GCATAGgcgg ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg 4201 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg 4261 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc 4321 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt 4381 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt 4441 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc 4501 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt 4561 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca 4621 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc 4681 gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggtg agcaagggcg 4741 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 4801 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 4861 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 4921 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 4981 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 5041 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca 5101 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 5161 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 5221 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 5281 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 5341 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 5401 actccaccgg cggcatggac gagctgtaca agggatctcg cgagggcaga ggaagtcttc 5461 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc 5521 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 5581 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 5641 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 5701 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 5761 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 5821 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 5881 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 5941 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 6001 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 6061 gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt 6121 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 6181 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 6241 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 6301 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 6361 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 6421 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 6481 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 6541 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 6601 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga 6661 tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 6721 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 6781 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 6841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 6901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 6961 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 7021 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 7081 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 7141 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 7201 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 7261 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 7321 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata 7381 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 7441 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 7501 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 7561 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7621 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 8041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 8101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 8161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 8221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 8281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 8341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 8461 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8521 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8581 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8641 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8701 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8761 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8821 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8881 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8941 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 9001 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 9061 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 9121 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 9181 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 9241 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 9301 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 9361 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 9421 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9481 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9541 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9601 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9661 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9721 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9781 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9841 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9901 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9961 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.