pCMV-USP37-3×Myc-Neo plasmid

pCMV-USP37-3×Myc-Neo plasmid

PVT95394

pCMV-USP37-3×Myc-Neo plasmid Information

Plasmid name: pCMV-USP37(human)-3×Myc-Neo
Alias: NM_020935.3;
Gene lenght: 2937bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-USP37-3×Myc-Neo plasmid Description

pCMV-USP37-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-USP37-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgtc tcctctgaag 721 atacatggtc ctatcagaat tcgaagtatg cagactggga ttacaaagtg gaaagaagga 781 tcctttgaaa ttgtagaaaa agagaataaa gtcagcctag tagttcacta caatactgga 841 ggaattccaa ggatatttca gctaagtcat aacattaaaa atgtggtgct tcgacccagt 901 ggagcgaaac aaagccgcct aatgttaact ctgcaagata acagcttctt gtctattgac 961 aaagtaccaa gtaaggatgc agaggaaatg aggttgtttc tagatgcagt ccatcaaaac 1021 agacttcctg cagccatgaa accgtctcag gggtctggta gttttggagc cattctgggc 1081 agcaggacct cacagaagga aaccagcagg cagctttctt actcagacaa tcaggcttct 1141 gcaaaaagag gaagtttgga aactaaagat gatattccat ttcgaaaagt tcttggtaat 1201 ccgggtagag gatcgattaa gactgtagca ggaagtggaa tagctcggac gattccttct 1261 ttgacatcta cttcaacacc tcttagatca gggttgctag aaaatcgtac tgaaaagagg 1321 aaaagaatga tatcaactgg ctcagaattg aatgaagatt accctaagga aaatgattca 1381 tcatcgaaca acaaggccat gacagatccc tccagaaagt atttaaccag cagtagagaa 1441 aagcagctga gtttgaaaca gtcagaagag aataggacat cagggctttt acctttacag 1501 tcatcatcct tttatggtag cagagctgga tccaaggaac actcttctgg tggcactaac 1561 ttagacagga ctaatgtttc aagccagact ccctctgcca aaagaagttt gggatttctt 1621 cctcagccag ttcctctttc tgttaaaaaa ctgaggtgta accaggatta cactggctgg 1681 aataaaccaa gagtgcccct ttcctctcac caacagcagc aactgcaggg cttctccaat 1741 ttgggaaata cctgctatat gaatgctatt ctacaatctc tattttcact ccagtcattt 1801 gcaaatgact tgcttaaaca aggtatccca tggaagaaaa ttccactcaa tgcacttatc 1861 agacgctttg cacacttgct tgttaaaaaa gatatctgta attcagagac caaaaaggat 1921 ttactcaaga aggttaaaaa tgccatttca gctacagcag agagattctc tggttatatg 1981 cagaatgatg ctcatgaatt tttaagtcag tgtttggacc agctgaaaga agatatggaa 2041 aaattaaata aaacttggaa gactgaacct gtttctggag aagaaaattc accagatatt 2101 tcagctacca gagcatacac ttgccctgtt attactaatt tggagtttga ggttcagcac 2161 tccatcattt gtaaagcatg tggagagatt atccccaaaa gagaacagtt taatgacctc 2221 tctattgacc ttcctcgtag gaaaaaacca ctccctcctc gttcaattca agattctctt 2281 gatcttttct ttagggccga agaactggag tattcttgtg agaagtgtgg tgggaagtgt 2341 gctcttgtca ggcacaaatt taacaggctt cctagggtcc tcattctcca tttgaaacga 2401 tatagcttca atgtggctct ctcgcttaac aataagattg ggcagcaagt catcattcca 2461 agatacctga ccctgtcatc tcattgcact gaaaatacaa aaccaccttt tacccttggt 2521 tggagtgcac atatggcaat ttctagacca ttgaaagcct ctcaaatggt gaattcctgc 2581 atcaccagcc cttctacacc ttcaaagaaa ttcaccttca aatccaagag ctccttggct 2641 ttatgccttg attcagacag tgaggatgag ctaaaacgtt ctgtggccct cagccagaga 2701 ctttgtgaaa tgttaggcaa cgaacagcag caggaagacc tggaaaaaga ttcaaaatta 2761 tgcccaatag agcctgacaa gtctgaattg gaaaactcag gatttgacag aatgagcgaa 2821 gaagagcttc tagcagctgt cttggagata agtaagagag atgcttcacc atctctgagt 2881 catgaagatg atgataagcc aactagcagc ccagataccg gatttgcaga agatgatatt 2941 caagaaatgc cagaaaatcc agacactatg gaaactgaga agcccaaaac aatcacagag 3001 ctggatcctg ccagttttac tgagataact aaagactgtg atgagaataa agaaaacaaa 3061 actccagaag gatctcaggg agaagttgat tggctccagc agtatgatat ggagcgtgaa 3121 agggaagagc aagagcttca gcaggcactg gctcagagcc ttcaagagca agaggcttgg 3181 gaacagaaag aagatgatga cctcaaaaga gctaccgagt taagtcttca agagtttaac 3241 aactcctttg tggatgcatt gggttctgat gaggactctg gaaatgagga tgtttttgat 3301 atggagtaca cagaagctga agctgaggaa ctgaaaagaa atgctgagac aggaaatctg 3361 cctcattcgt accggctcat cagtgttgtc agtcacattg gtagcacttc ttcttcaggt 3421 cattacatta gtgatgtata tgacattaag aagcaagcgt ggtttactta caatgacctg 3481 gaggtatcaa aaatccaaga ggctgccgtg cagagtgatc gagatcggag tggctacatc 3541 ttcttttata tgcacaagga gatctttgat gagctgctgg aaacagaaaa gaactctcag 3601 tcacttagca cggaagtggg gaagactacc cgtcaggcct tgCTCGAGGA ACAAAAACTA 3661 ATATCAGAGG AAGATTTGGA ACAGAAACTA ATCAGTGAAG AAGATTTAGA GCAAAAGTTA 3721 ATTTCCGAGG AGGACTTATA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA 3781 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 3841 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 3901 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 3961 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 4021 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 4081 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 4141 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 4201 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 4261 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 4321 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG 4381 GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC 4441 GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG 4501 TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC 4561 CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 4621 CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG 4681 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 4741 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 4801 CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 4861 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 4921 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 4981 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA 5041 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT 5101 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 5161 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 5221 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC 5281 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 5341 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 5401 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 5461 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG 5521 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 5581 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 5641 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 5701 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 5761 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 5821 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 5881 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 5941 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 6001 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 6061 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 6121 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG 6181 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT 6241 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC 6301 GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 6361 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 6421 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 6481 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 6541 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 6601 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 6661 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 6721 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 6781 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 6841 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 6901 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 6961 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 7021 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 7081 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 7141 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 7201 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC 7261 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 7321 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 7381 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 7441 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 7501 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 7561 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 7621 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 7681 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 7741 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 7801 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 7861 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 7921 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 7981 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 8041 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 8101 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 8161 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 8221 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 8281 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 8341 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 8401 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 8461 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 8521 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 8581 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 8641 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 8701 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 8761 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC 8821 CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA 8881 TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC 8941 AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.