pTac-GST-CGAS plasmid

pTac-GST-CGAS plasmid

PVT95383

pTac-GST-CGAS plasmid Information

Plasmid name: pTac-GST-CGAS(human)
Alias: NM_138441.3;C6orf150; D4; h-cGAS; MB21D1
Gene lenght: 1569bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-CGAS plasmid Description

pTac-GST-CGAS is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-CGAS plasmid Sequence

1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg atcgaaggtc gtgggatccC Catgcagcct tggcacggaa 961 aggccatgca gagagcttcc gaggccggag ccactgcccc caaggcttcc gcacggaatg 1021 ccaggggcgc cccgatggat cccaccgagt ctccggctgc ccccgaggcc gccctgccta 1081 aggcgggaaa gttcggcccc gccaggaagt cgggatcccg gcagaaaaag agcgccccgg 1141 acacccagga gaggccgccc gtccgcgcaa ctggggcccg cgccaaaaag gcccctcagc 1201 gcgcccagga cacgcagccg tctgacgcca ccagcgcccc tggggcagag gggctggagc 1261 ctcctgcggc tcgggagccg gctctttcca gggctggttc ttgccgccag aggggcgcgc 1321 gctgctccac gaagccaaga cctccgcccg ggccctggga cgtgcccagc cccggcctgc 1381 cggtctcggc ccccattctc gtacggaggg atgcggcgcc tggggcctcg aagctccggg 1441 cggttttgga gaagttgaag ctcagccgcg atgatatctc cacggcggcg gggatggtga 1501 aaggggttgt ggaccacctg ctgctcagac tgaagtgcga ctccgcgttc agaggcgtcg 1561 ggctgctgaa caccgggagc tactatgagc acgtgaagat ttctgcacct aatgaatttg 1621 atgtcatgtt taaactggaa gtccccagaa ttcaactaga agaatattcc aacactcgtg 1681 catattactt tgtgaaattt aaaagaaatc cgaaagaaaa tcctctgagt cagtttttag 1741 aaggtgaaat attatcagct tctaagatgc tgtcaaagtt taggaaaatc attaaggaag 1801 aaattaacga cattaaagat acagatgtca tcatgaagag gaaaagagga gggagccctg 1861 ctgtaacact tcttattagt gaaaaaatat ctgtggatat aaccctggct ttggaatcaa 1921 aaagtagctg gcctgctagc acccaagaag gcctgcgcat tcaaaactgg ctttcagcaa 1981 aagttaggaa gcaactacga ctaaagccat tttaccttgt acccaagcat gcaaaggaag 2041 gaaatggttt ccaagaagaa acatggcggc tatccttctc tcacatcgaa aaggaaattt 2101 tgaacaatca tggaaaatct aaaacgtgct gtgaaaacaa agaagagaaa tgttgcagga 2161 aagattgttt aaaactaatg aaataccttt tagaacagct gaaagaaagg tttaaagaca 2221 aaaaacatct ggataaattc tcttcttatc atgtgaaaac tgccttcttt cacgtatgta 2281 cccagaaccc tcaagacagt cagtgggacc gcaaagacct gggcctctgc tttgataact 2341 gcgtgacata ctttcttcag tgcctcagga cagaaaaact tgagaattat tttattcctg 2401 aattcaatct attctctagc aacttaattg acaaaagaag taaggaattt ctgacaaagc 2461 aaattgaata tgaaagaaac aatgagtttc cagtttttga tgaattttga gaattcccgg 2521 gtcgactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg 2581 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 2641 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 2701 cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc 2761 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 2821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 2881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 2941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 3001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 3061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 3121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 3181 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 3241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 3301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 3361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 3421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 3481 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 3541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 3601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 3661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 3721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 3781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 3841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 3901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 3961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 4021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 4081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 4141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 4201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 4261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 4321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 4381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 4441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 4501 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 4561 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 4621 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 4681 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 4741 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 4801 gcataaattc cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc 4861 cggaagagag tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag 4921 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt 4981 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc 5041 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc 5101 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg 5161 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg 5221 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc 5281 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct 5341 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg 5401 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt 5461 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc 5521 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc 5581 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc 5641 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag 5701 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac 5761 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc 5821 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg 5881 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 5941 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 6001 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 6061 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc 6121 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 6181 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 6241 ccaacagttg cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc 6301 ggtgccggaa agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc 6361 aaactggcag atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac 6421 ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa 6481 tgttgatgaa agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttggaat 6541 t

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.