pCMV-TRIM28-Del(147-245aa-3×HA

pCMV-TRIM28-Del(147-245aa-3×HA-Neo plasmid

PVT95361

pCMV-TRIM28-Del(147-245aa-3×HA-Neo plasmid Information

Plasmid name: pCMV-TRIM28(human)-Del(147-245aa)-3×HA-Neo
Alias:
Gene lenght: 2208bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pCMV-TRIM28-Del(147-245aa-3×HA-Neo plasmid Description

pCMV-TRIM28-Del(147-245aa-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TRIM28-Del(147-245aa-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCg ccaccatggc ggcctccgcg 721 gcggcagcct cggcagcagc ggcctcggcc gcctctggca gcccgggccc gggcgagggc 781 tccgctggcg gcgaaaagcg ctccaccgcc ccttcggccg cagcctcggc ctctgcctca 841 gccgcggcgt cgtcgcccgc ggggggcggc gccgaggcgc tggagctgct ggagcactgc 901 ggcgtgtgca gagagcgcct gcgacccgag agggagcccc gcctgctgcc ctgtttgcac 961 tcggcctgta gtgcctgctt agggcccgcg gcccccgccg ccgccaacag ctcgggggac 1021 ggcggggcgg cgggcgacgg caccgtggtg gactgtcccg tgtgcaagca acagtgcttc 1081 tccaaagaca tcgtggagaa ttatttcatg cgtgatagtg gcagcaaggc tgccaccgac 1141 gccgaggatg cagtgaggaa ccagcgcaag ctcctggcct cactggtgaa gcgccttggg 1201 gacaaacatg caacattgca gaagagcacc aaggaggttc gcagctcaat ccgccaggtg 1261 tctgacgtac agaagcgtgt gcaagtggat gtcaagatgg ccatcctgca gatcatgaag 1321 gagctgaata agcggggccg tgtgctggtc aatgatgccc agaaggtgac tgaggggcag 1381 caggagcgcc tggagcggca gcactggacc atgaccaaga tccagaagca ccaggagcac 1441 attctgcgct ttgcctcttg ggctctggag agtgacaaca acacagccct tttgctttct 1501 aagaagttga tctacttcca gctgcaccgg gccctcaaga tgattgtgga tcccgtggag 1561 ccacatggcg agatgaagtt tcagtgggac ctcaatgcct ggaccaagag tgccgaggcc 1621 tttggcaaga ttgtggcaga gcgtcctggc actaactcaa caggccctgc acccatggcc 1681 cctccaagag ccccagggcc cctgagcaag cagggctctg gcagcagcca gcccatggag 1741 gtgcaggaag gctatggctt tgggtcagga gatgatccct actcaagtgc agagccccat 1801 gtgtcaggtg tgaaacggtc ccgctcaggt gagggcgagg tgagcggcct tatgcgcaag 1861 gtgccacgag tgagccttga acgcctggac ctggacctca cagctgacag ccagccaccc 1921 gtcttcaagg tcttcccagg cagtaccact gaggactaca accttattgt tattgaacgt 1981 ggcgctgccg ctgcagctac cggccagcca gggactgcgc ctgcaggaac ccctggtgcc 2041 ccacccctgg ctggcatggc cattgtcaag gaggaggaga cggaggctgc cattggagcc 2101 cctcctactg ccactgaggg ccctgagacc aaacctgtgc ttatggctct tgcggagggt 2161 cctggtgctg agggtccccg cctggcctca cctagtggca gcaccagctc agggctggag 2221 gtggtggctc ctgagggtac ctcagcccca ggtggtggcc cgggaaccct ggatgacagt 2281 gccaccattt gccgtgtctg ccagaagcca ggcgatctgg ttatgtgcaa ccagtgtgag 2341 ttttgtttcc acctggactg tcacctgccg gccctgcagg atgtaccagg ggaggagtgg 2401 agctgctcac tctgccatgt gctccctgac ctgaaggagg aggatggcag cctcagcctg 2461 gatggtgcag acagcactgg cgtggtggcc aagctctcac cagccaacca gcggaaatgt 2521 gagcgtgtac tgctggccct attctgtcac gaaccctgcc gccccctgca tcagctggct 2581 accgactcca ccttctccct ggaccagccc ggtggcaccc tggatctgac cctgatccgt 2641 gcccgcctcc aggagaagtt gtcacctccc tacagctccc cacaggagtt tgcccaggat 2701 gtgggccgca tgttcaagca attcaacaag ttaactgagg acaaggcaga cgtgcagtcc 2761 atcatcggcc tgcagcgctt cttcgagacg cgcatgaacg aggccttcgg tgacaccaag 2821 ttctctgctg tgctggtgga gcccccgccg atgagcctgc ctggtgctgg cctgagttcc 2881 caggagctgt ctggtggccc tggtgatggc cccCTCGAGT ACCCATACGA CGTACCTGAT 2941 TATGCAACTT ATCCTTATGA TGTACCTGAT TATGCTAGCT ATCCTTATGA TGTCCCAGAC 3001 TACGCATAGT GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA 3061 GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA 3121 TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT 3181 CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT 3241 GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC 3301 TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC 3361 TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG 3421 GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA 3481 CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC 3541 TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC 3601 TTTGGGCCGC CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT 3661 ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG 3721 GGTCGTTTGT TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC 3781 CGAGACCCCA TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA 3841 GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG 3901 ATCTGCGCAG CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG 3961 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 4021 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 4081 TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 4141 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 4201 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 4261 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT 4321 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG 4381 TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA 4441 TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 4501 GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC 4561 GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT 4621 TTATGCAGAG GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT 4681 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC 4741 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 4801 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 4861 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 4921 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 4981 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 5041 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 5101 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 5161 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 5221 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 5281 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 5341 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 5401 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA 5461 AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 5521 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG 5581 TTCGCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 5641 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 5701 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 5761 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 5821 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 5881 CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 5941 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 6001 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 6061 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 6121 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 6181 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 6241 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 6301 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 6361 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 6421 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 6481 TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT 6541 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 6601 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 6661 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 6721 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 6781 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 6841 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 6901 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 6961 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 7021 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 7081 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 7141 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 7201 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 7261 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 7321 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 7381 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 7441 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 7501 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 7561 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 7621 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 7681 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 7741 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 7801 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 7861 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 7921 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 7981 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 8041 CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG 8101 GTCGACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC 8161 TTGTGTGTTG GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG 8221 CTTGACCGAC AATTGCATGA AGAATCTGCT TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.