pTac-GST-EGFP-MCS plasmid

pTac-GST-EGFP-MCS plasmid

PVT95231

pTac-GST-EGFP-MCS plasmid Information

Plasmid name: pTac-GST-EGFP-MCS
Alias:
Gene lenght:
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-EGFP-MCS plasmid Description

pTac-GST-EGFP-MCS is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-EGFP-MCS plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggt gagcaagggc gaggagctgt 961 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca 1021 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 1081 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 1141 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 1201 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 1261 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 1321 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 1381 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 1441 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 1501 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 1561 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 1621 ggatcactct cggcatggac gagctgtaca agggcggtgg aggcagtggt ggaggcggta 1681 gcctcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg 1741 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 1801 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 1861 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg 1921 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 1981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 2041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 2101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 2161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 2221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 2281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 2341 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 2401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 2461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 2521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 2581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 2641 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 2701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 2761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 2821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 2881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 2941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 3001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 3061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 3121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 3181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 3241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 3301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 3361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 3421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 3481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 3541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 3601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 3661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 3721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 3781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 3841 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 3901 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 3961 taaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg 4021 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt 4081 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg 4141 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg 4201 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg 4261 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg 4321 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc 4381 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg 4441 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg 4501 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg 4561 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg 4621 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc 4681 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa 4741 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg 4801 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg 4861 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg 4921 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg 4981 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc 5041 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 5101 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 5161 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 5221 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt 5281 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 5341 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 5401 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt 5461 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa 5521 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt 5581 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt 5641 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.