pCMV-KIF15-3×Myc-Neo plasmid

pCMV-KIF15-3×Myc-Neo plasmid

PVT95210

pCMV-KIF15-3×Myc-Neo plasmid Information

Plasmid name: pCMV-KIF15(human)-3×Myc-Neo
Alias: NM_020242.3;BRDCS2; HKLP2; KLP2; KNSL7; NY-BR-62
Gene lenght: 4164bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-KIF15-3×Myc-Neo plasmid Description

pCMV-KIF15-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-KIF15-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc acccggctgc 721 aaaactgagt tacgcagcgt gacaaatggt cagtctaacc aaccaagtaa tgaaggtgat 781 gccatcaaag tttttgtgcg aattcgtcct cctgcagaaa gatctgggtc agctgatgga 841 gagcagaact tatgcttatc tgtgctgtcc tccacgagtc tccggctgca ctccaaccct 901 gagcccaaga ccttcacgtt tgatcatgtt gcagatgtgg ataccactca ggaatctgta 961 ttcgcaactg tggctaaaag cattgtggag tcttgcatga gcggttataa tggtaccatc 1021 tttgcatatg gacagactgg ctcagggaag acatttacta tgatgggacc atctgaatct 1081 gataattttt ctcataacct gagaggagta atcccacgaa gttttgaata tttgttttcc 1141 ttaattgatc gtgaaaaaga aaaggctgga gctggaaaga gtttcctttg taagtgttcc 1201 tttattgaaa tctacaacga gcagatatat gatctactgg actctgcatc ggctggactg 1261 tacttaaggg agcatatcaa gaagggagtc tttgttgttg gtgcggtgga gcaggtggta 1321 acctcagctg ctgaagccta tcaggtgttg tctggaggat ggaggaatag acgtgtggca 1381 tcaacatcaa tgaacagaga atcgtctagg tctcatgccg tctttacaat tacaatagag 1441 tcaatggaga aaagtaatga gattgtgaat atacggacct ccctactcaa cctggtggat 1501 ttagcaggat ctgaaaggca aaaagatacc catgcagaag ggatgagatt gaaggaagca 1561 ggtaacataa atcgatcatt gagctgcctg ggccaagtga ttacagcact tgtcgacgtg 1621 ggtaatggaa aacagagaca tgtttgctac agagactcca aacttacctt cttactacgg 1681 gattcccttg gaggtaatgc caaaacagcc ataattgcaa atgttcatcc tggatccagg 1741 tgttttgggg aaaccctatc aacacttaac tttgctcaaa gagccaagct gattaaaaac 1801 aaggcagtag taaatgaaga cacccaagga aatgtgagcc agctccaagc tgaagtgaag 1861 aggctcaaag aacaactggc ggagcttgct tcaggacaga caccaccaga aagcttcctg 1921 accagagaca aaaagaagac taactatatg gagtatttcc aggaagcaat gttattcttt 1981 aagaaatctg aacaggaaaa gaagtctctg atagaaaaag ttacccaatt agaagacctc 2041 accctcaaaa aggaaaaatt tattcaatct aataaaatga ttgtgaaatt ccgagaggat 2101 caaataatac gcttggaaaa gctccacaag gaatcccggg gaggttttct gcctgaggag 2161 caggatcgtt tgctctcaga attaaggaat gagattcaaa ctctgcgaga acaaatagag 2221 caccacccca gagttgcaaa gtatgctatg gaaaatcatt ccctcaggga ggagaataga 2281 agactgagat tattagagcc tgtgaaaaga gctcaagaaa tggatgccca gaccattgca 2341 aaactagaaa aagctttctc tgaaataagt ggcatggaga aaagtgacaa aaatcagcaa 2401 ggattttcac ctaaagctca gaaagagcca tgtttgtttg caaacactga gaagttaaaa 2461 gcacaactcc tgcaaattca gacagagctg aataattcaa agcaagaata tgaagaattc 2521 aaagaactta ctaggaaaag gcagctagaa ttggaatcag agcttcagtc tttgcaaaaa 2581 gcgaacctta atcttgaaaa ccttttggaa gcaacaaaag cctgcaagcg gcaagaagtt 2641 tctcagctga ataaaattca tgctgaaaca cttaagatta taactacacc aaccaaggcc 2701 taccaacttc attcccgacc agtaccaaaa ttaagccctg aaatgggaag ctttggctct 2761 ctatacactc agaattctag catattagat aatgatatat taaatgagcc agttcctcct 2821 gagatgaatg aacaagcttt tgaggccatt tctgaagagc ttagaacagt gcaggaacaa 2881 atgagtgctc ttcaagccaa actggatgaa gaagagcata aaaacctaaa gcttcagcag 2941 catgttgaca aactggaaca tcattctacc caaatgcagg agcttttctc atcagaaaga 3001 attgattgga ccaaacagca ggaagagctt ctctcacagt tgaatgtcct tgaaaagcag 3061 cttcaagaga ctcaaactaa aaatgacttt ttgaaaagtg aggtacatga cctgcgagta 3121 gtccttcatt ctgctgacaa ggagctttct tcagtgaaat tggaatatag ttcattcaaa 3181 acgaatcagg agaaagaatt caacaaactt tccgaaagac acatgcatgt acagcttcaa 3241 ttagataatc tcaggttaga aaacgaaaag ctgcttgaga gcaaagcctg cctacaggat 3301 tcctatgaca acttacaaga aataatgaaa tttgagattg accaactttc aagaaacctc 3361 caaaacttca aaaaagaaaa tgaaactctg aaatctgatc tgaataattt gatggagctt 3421 cttgaggcag aaaaagaacg caataacaaa ttatcattac agtttgaaga agataaagaa 3481 aacagttcta aagaaatctt aaaagttctt gaggctgtac gtcaggagaa acagaaagag 3541 acggccaagt gtgagcagca gatggcaaaa gtacagaaac tagaagagag cttgcttgct 3601 actgaaaaag tgatcagttc cctggaaaag tctagagatt ctgataagaa agttgtagct 3661 gacctcatga accagatcca ggagctaaga acatcggtct gtgagaaaac agaaactata 3721 gacaccctga aacaagaact gaaggacata aattgcaaat acaactctgc tttggttgac 3781 agagaagaga gcagagtgtt gatcaagaag caggaagtgg atattctgga tctgaaagaa 3841 acccttaggc tgagaatact ttctgaggac atagagaggg atatgctctg tgaggacctg 3901 gctcatgcca ctgagcagct gaacatgctc acagaggcct caaaaaaaca ctcggggctg 3961 ctgcagtctg cccaggaaga actgaccaag aaggaagccc tgattcagga acttcagcac 4021 aagctaaacc aaaagaaaga ggaagtagaa cagaagaaga atgaatataa cttcaaaatg 4081 aggcaactag aacatgtgat ggattctgct gctgaggatc cccagagtcc taagacacca 4141 cctcactttc aaacacattt ggcaaaactc ctggaaacac aagaacaaga gatagaagat 4201 ggaagagcct ctaagacttc tttggaacac cttgtaacaa agctaaatga agacagagaa 4261 gtcaaaaatg ctgaaatcct cagaatgaag gagcagttgc gtgaaatgga aaacctacgc 4321 ctggaaagtc agcagttaat agagaaaaac tggctcctgc aaggtcagct ggatgatatt 4381 aaaagacaaa aggaaaacag tgatcagaat catccagata atcaacagct gaagaatgaa 4441 caagaagaaa gtatcaaaga aagacttgca aaaagtaaaa tagttgaaga aatgctgaaa 4501 atgaaagcag acctagaaga agtccaaagt gccctttaca acaaagagat ggaatgcctt 4561 agaatgactg atgaagtcga acgaacccaa actttggagt ctaaagcatt ccaggaaaaa 4621 gaacaactga gatcaaagct ggaagaaatg tatgaagaaa gagagagaac atcccaggag 4681 atggaaatgt taaggaagca ggtggagtgt cttgctgagg aaaatggaaa gttggtaggt 4741 caccaaaatt tgcatcagaa gattcagtac gtagtgcgac taaagaagga aaatgtcagg 4801 cttgctgagg agacagaaaa gttgcgtgcc gaaaatgtat ttttaaaaga aaagaaaaga 4861 agtgaatctC TCGAGGAACA AAAACTAATA TCAGAGGAAG ATTTGGAACA GAAACTAATC 4921 AGTGAAGAAG ATTTAGAGCA AAAGTTAATT TCCGAGGAGG ACTTATAGTG AGGGCCCGAT 4981 ATCTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT 5041 ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG 5101 CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG 5161 AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA 5221 CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC 5281 CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG 5341 CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT 5401 GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT 5461 CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC 5521 CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG 5581 GAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT 5641 GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG 5701 GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT 5761 ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC 5821 TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA TCTGCGCAGC TGGGGCTCTA 5881 GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC 5941 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT 6001 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG 6061 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT 6121 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 6181 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT 6241 CTTTTGATTT ATAAGGGATT TTGGGGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT 6301 AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC 6361 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG 6421 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA 6481 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC 6541 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC 6601 CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 6661 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 6721 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 6781 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 6841 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 6901 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 6961 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 7021 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 7081 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 7141 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 7201 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 7261 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 7321 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 7381 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 7441 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 7501 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGCGAAATG ACCGACCAAG 7561 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 7621 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 7681 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 7741 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 7801 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 7861 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 7921 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 7981 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 8041 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT 8101 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 8161 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 8221 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 8281 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 8341 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 8401 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 8461 TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 8521 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 8581 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 8641 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 8701 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 8761 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 8821 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 8881 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 8941 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 9001 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 9061 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 9121 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC 9181 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 9241 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 9301 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 9361 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 9421 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 9481 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 9541 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 9601 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 9661 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 9721 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 9781 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 9841 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 9901 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 9961 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 10021 CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG TCGACTCTCA GTACAATCTG 10081 CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA 10141 GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA 10201 GAATCTGCTT AGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.