pLV3-CMV-UBE2L6-3×HA-CopGFP-Pu

pLV3-CMV-UBE2L6-3×HA-CopGFP-Puro plasmid

PVT94949

pLV3-CMV-UBE2L6-3×HA-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-UBE2L6(human)-3×HA-CopGFP-Puro
Alias: NM_004223.5;RIG-B; UBCH8
Gene lenght: 459bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-UBE2L6-3×HA-CopGFP-Puro plasmid Description

pLV3-CMV-UBE2L6-3×HA-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-UBE2L6-3×HA-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tggcgagcat gcgagtggtg 2341 aaggagctgg aggatcttca gaagaagcct cccccatacc tgcggaacct gtccagcgat 2401 gatgccaatg tcctggtgtg gcacgctctc ctcctacccg accaacctcc ctaccacctg 2461 aaagccttca acctgcgcat cagcttcccg ccggagtatc cgttcaagcc tcccatgatc 2521 aaattcacaa ccaagatcta ccaccccaac gtggacgaga acggacagat ttgcctgccc 2581 atcatcagca gtgagaactg gaagccttgc accaagactt gccaagtcct ggaggccctc 2641 aatgtgctgg tgaatagacc gaatatcagg gagcccctgc ggatggacct cgctgacctg 2701 ctgacacaga atccggagct gttcagaaag aatgccgaag agttcaccct ccgattcgga 2761 gtggaccggc cctccCTCGA GTACCCATAC GACGTACCTG ATTATGCAAC TTATCCTTAT 2821 GATGTACCTG ATTATGCTAG CTATCCTTAT GATGTCCCAG ACTACGCATA GggatccCTG 2881 CGATATCCAG CgcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 2941 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 3001 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 3061 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 3121 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 3181 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 3241 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 3301 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 3361 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 3421 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 3481 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 3541 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 3601 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 3661 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 3721 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 3781 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 3841 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 3901 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 3961 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 4021 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 4081 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 4141 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 4201 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 4261 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 4321 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 4381 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 4441 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 4501 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 4561 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 4621 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 4681 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 4741 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 4801 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 4861 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 4921 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 4981 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 5041 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 5101 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 5161 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 5221 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 5281 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 5341 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 5401 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg 5461 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5521 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt 5581 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 5641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 5701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 5761 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 5821 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 5881 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 5941 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 6001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 6061 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt 6121 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6181 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 6241 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6301 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6361 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6421 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6481 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6541 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6601 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6661 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6721 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6781 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6841 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 6901 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 6961 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7021 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7081 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7141 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7201 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7261 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7321 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7381 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7441 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7501 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7561 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7621 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7681 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7741 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7801 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7861 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 7921 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 7981 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8041 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8101 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8161 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8221 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8281 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 8341 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 8401 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 8461 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 8521 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 8581 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 8641 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 8701 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 8761 ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.