pLV3-CMV-FOXK1-3×FLAG-Neo plas

pLV3-CMV-FOXK1-3×FLAG-Neo plasmid

PVT94896

pLV3-CMV-FOXK1-3×FLAG-Neo plasmid Information

Plasmid name: pLV3-CMV-FOXK1(human-gc-opt)-3×FLAG-Neo
Alias: NM_001037165.2;FOXK1L
Gene lenght: 2199bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FOXK1-3×FLAG-Neo plasmid Description

pLV3-CMV-FOXK1-3×FLAG-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-FOXK1-3×FLAG-Neo plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac cATGGCTGAG GTGGGAGAGG ACAGCGGAGC TAGGGCTCTG 2341 CTGGCTCTGA GGAGCGCTCC TTGCAGCCCT GTGCTGTGCG CTGCTGCTGC TGCCGCTGCT 2401 TTCCCTGCTG CTGCTCCTCC TCCTGCTCCT GCTCAGCCTC AGCCTCCTCC TGGACCTCCT 2461 CCTCCTCCCC CTCCTCCTCT GCCTCCTGGA GCTATCGCTG GAGCTGGAAG CAGCGGAGGA 2521 AGCAGCGGCG TGAGCGGAGA CAGCGCTGTG GCTGGAGCTG CTCCTGCTCT GGTGGCTGCT 2581 GCTGCTGCTA GCGTGAGGCA GAGCCCTGGA CCTGCTCTGG CTAGGCTGGA GGGAAGGGAG 2641 TTCGAGTTCC TGATGAGGCA GCCTAGCGTG ACCATCGGAA GGAACAGCAG CCAGGGAAGC 2701 GTGGACCTGA GCATGGGACT GAGCAGCTTC ATCAGCAGGA GGCACCTGCA GCTGAGCTTC 2761 CAGGAGCCTC ACTTCTACCT GAGGTGCCTG GGAAAGAATG GAGTGTTTGT GGACGGAGCT 2821 TTCCAGAGGA GGGGAGCTCC TGCTCTGCAG CTGCCTAAGC AGTGCACCTT CAGGTTCCCT 2881 AGCACCGCTA TCAAAATCCA GTTCACCAGC CTGTACCACA AGGAAGAAGC TCCTGCTTCT 2941 CCACTGAGAC CTCTGTACCC TCAGATTTCT CCTCTGAAGA TCCACATCCC CGAGCCTGAC 3001 CTGAGGAGCA TGGTGAGCCC TGTGCCTAGC CCTACCGGAA CCATCAGCGT GCCTAACAGC 3061 TGCCCTGCTA GCCCTAGGGG AGCTGGATCT AGCAGCTACA GGTTCGTGCA GAATGTGACA 3121 AGCGACCTGC AGCTGGCTGC TGAGTTCGCT GCTAAGGCTG CTAGCGAGCA GCAGGCTGAC 3181 ACCAGCGGAG GAGACAGCCC TAAGGACGAG AGCAAGCCTC CTTTCAGCTA TGCTCAGCTG 3241 ATCGTGCAGG CTATTAGCAG CGCTCAGGAC AGGCAGCTGA CCCTGAGCGG AATCTACGCT 3301 CACATCACCA AACACTACCC CTACTACAGG ACCGCTGATA AAGGATGGCA GAACTCTATC 3361 CGGCACAACC TGAGCCTGAA TAGATACTTC ATCAAGGTGC CTAGGAGCCA GGAAGAACCT 3421 GGAAAGGGAA GCTTTTGGAG GATCGATCCA GCTTCTGAGG CTAAACTGGT GGAACAGGCT 3481 TTCAGAAAGC GGAGGCAGAG GGGAGTGAGC TGCTTCAGGA CCCCTTTCGG ACCTCTGAGC 3541 AGCAGGAGCG CTCCCGCTAG CCCTACCCAC CCTGGACTGA TGAGCCCTAG GAGCGGAGGA 3601 CTGCAGACCC CTGAGTGCCT GAGCAGGGAG GGAAGCCCTA TCCCTCACGA CCCTGAGTTC 3661 GGAAGCAAGC TGGCTAGCGT GCCTGAGTAC AGGTACAGCC AGAGCGCTCC TGGAAGCCCT 3721 GTGAGCGCTC AGCCTGTGAT CATGGCTGTG CCTCCTAGGC CTAGCAGCCT GGTGGCTAAG 3781 CCTGTGGCTT ACATGCCTGC TAGCATTGTG ACAAGCCAGC AGCCTGCTGG ACACGCTATC 3841 CACGTGGTGC AGCAGGCTCC TACCGTGACC ATGGTGAGGG TGGTGACCAC CAGCGCTAAC 3901 AGCGCTAACG GATACATTCT GACCAGCCAG GGAGCTGCTG GAGGAAGCCA CGACGCTGCT 3961 GGAGCTGCCG TGCTGGACCT GGGAAGCGAG GCTAGGGGAC TGGAGGAGAA GCCTACCATC 4021 GCTTTCGCTA CCATTCCTGC TGCTGGAGGC GTGATCCAGA CCGTGGCTAG CCAGATGGCT 4081 CCTGGAGTGC CTGGACACAC CGTGACCATC CTGCAGCCTG CTACCCCTGT GACCCTGGGA 4141 CAGCACCACC TGCCTGTGAG GGCTGTGACC CAGAACGGAA AGCACGCTGT GCCTACCAAC 4201 AGCCTGGCTG GAAACGCTTA CGCTCTGACC AGCCCTCTGC AGCTGCTGGC TACCCAGGCT 4261 AGCAGCAGCG CTCCTGTGGT GGTGACCAGG GTGTGCGAGG TGGGACCTAA GGAGCCTGCT 4321 GCTGCCGTGG CTGCTACCGC TACCACCACC CCTGCTACCG CTACAACCGC TAGCGCTAGC 4381 GCTTCCAGCA CCGGAGAGCC TGAGGTGAAG AGGAGCAGGG TGGAGGAGCC TAGCGGAGCT 4441 GTGACCACCC CTGCCGGAGT GATCGCTGCT GCTGGACCTC AGGGACCTGG AACCGGAGAG 4501 GGATCCGACT ACAAAGACCA TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT 4561 GACGATGACA AGTAGCGGCC GCGAAGGATC TGCGATCGCT CCGGTGCCCG TCAGTGGGCA 4621 GAGCGCACAT CGCCCACAGT CCCCGAGAAG TTGGGGGGAG GGGTCGGCAA TTGAACGGGT 4681 GCCTAGAGAA GGTGGCGCGG GGTAAACTGG GAAAGTGATG TCGTGTACTG GCTCCGCCTT 4741 TTTCCCGAGG GTGGGGGAGA ACCGTATATA AGTGCAGTAG TCGCCGTGAA CGTTCTTTTT 4801 CGCAACGGGT TTGCCGCCAG AACACAGCTG AAGCTTCGAG GGGCTCGCAT CTCTCCTTCA 4861 CGCGCCCGCC GCCCTACCTG AGGCCGCCAT CCACGCCGGT TGAGTCGCGT TCTGCCGCCT 4921 CCCGCCTGTG GTGCCTCCTG AACTGCGTCC GCCGTCTAGG TAAGTTTAAA GCTCAGGTCG 4981 AGACCGGGCC TTTGTCCGGC GCTCCCTTGG AGCCTACCTA GACTCAGCCG GCTCTCCACG 5041 CTTTGCCTGA CCCTGCTTGC TCAACTCTAC GTCTTTGTTT CGTTTTCTGT TCTGCGCCGT 5101 TACAGATCCA AGCTGTGACC GGCGCCTACG CTAGGCCACC atggctgaac aagatggatt 5161 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 5221 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 5281 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 5341 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 5401 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 5461 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 5521 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 5581 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 5641 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 5701 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 5761 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 5821 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 5881 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaGTCGA 5941 CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 6001 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 6061 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 6121 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 6181 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 6241 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 6301 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT 6361 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 6421 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 6481 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGTACCTT 6541 TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG 6601 GACTGGAAGG GCTAATTCAC TCCCAACGAA AATAAGATCT GCTTTTTGCT TGTACTGGGT 6661 CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC 6721 TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG 6781 ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA 6841 GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA 6901 GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 6961 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 7021 ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 7081 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC 7141 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGACT TTTGCAGAGA 7201 CGGCCCAAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 7261 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 7321 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 7381 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 7441 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 7501 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 7561 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 7621 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 7681 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 7741 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 7801 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 7861 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 7921 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 7981 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 8041 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 8101 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 8161 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 8221 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 8281 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 8341 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 8401 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 8461 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 8521 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 8581 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 8641 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 8701 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 8761 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 8821 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 8881 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 8941 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 9001 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 9061 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 9121 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 9181 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 9241 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 9301 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 9361 AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG 9421 GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA 9481 CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC 9541 CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC 9601 AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 9661 GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG 9721 ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG 9781 ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGC 9841 CAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.