pCMV-SMARCC1-NLS-3×HA-Neo plas

pCMV-SMARCC1-NLS-3×HA-Neo plasmid

PVT94859

pCMV-SMARCC1-NLS-3×HA-Neo plasmid Information

Plasmid name: pCMV-SMARCC1(human)-NLS-3×HA-Neo
Alias: NM_003074.4;BAF155; CRACC1; HYC5; Rsc8; SRG3; SWI3
Gene lenght: 3369bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-SMARCC1-NLS-3×HA-Neo plasmid Description

pCMV-SMARCC1-NLS-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-SMARCC1-NLS-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCg ccaccatggc cgcagcggcg 721 ggcggcggcg ggccggggac agcggtaggc gccacgggct cggggattgc ggcggcagcc 781 gcaggcctag ctgtttatcg acggaaggat gggggcccgg ccaccaagtt ttgggagagc 841 ccggagacgg tgtcccagct ggattcggtg cgggtctggc tgggcaagca ctacaagaag 901 tatgttcatg cggatgctcc taccaataaa acactggctg ggctggtggt gcagcttctt 961 cagttccagg aagatgcctt tgggaagcat gtcaccaacc cggccttcac caaactccct 1021 gcaaagtgtt tcatggattt caaagctgga ggcgccttat gtcacattct tggggctgct 1081 tacaagtata aaaatgaaca gggatggcgg aggtttgacc tacagaaccc atctcgaatg 1141 gatcgtaatg tggaaatgtt tatgaacatt gaaaaaacat tggtgcagaa caattgtttg 1201 accagaccca acatctacct cattccagac attgatctga agttggctaa caaattgaaa 1261 gatatcatca aacgacatca gggaacattt acggatgaga agtcaaaagc ttcccaccac 1321 atttacccat attcttcctc acaagacgat gaagaatggt tgagaccggt gatgagaaaa 1381 gagaagcaag tgttagtgca ttggggcttt tacccagaca gctatgatac ttgggtccat 1441 agtaatgatg ttgatgctga aattgaagat ccaccaattc cagaaaaacc atggaaggtt 1501 catgtgaaat ggattttgga cactgatatt ttcaatgaat ggatgaatga ggaggattat 1561 gaggtggatg aaaataggaa gcctgtgagt tttcgtcagc ggatttcaac caagaatgaa 1621 gagccagtca gaagtccaga aagaagagat agaaaagcat cagctaatgc tcgaaagagg 1681 aaacattcgc cttcgcctcc ccctccgaca ccaacagaat cacggaagaa gagtgggaag 1741 aaaggccaag ctagccttta tgggaagcgc agaagtcaga aagaggaaga tgagcaagaa 1801 gatctaacca aggatatgga agacccaaca cctgtaccca atatagaaga agtagtactt 1861 cccaaaaatg tgaacctaaa gaaagatagt gaaaatacac ctgttaaagg aggaactgta 1921 gcggatctag atgagcagga tgaagaaaca gtcacagcag gaggaaagga agatgaagat 1981 cctgccaaag gtgatcagag tcgatcagtt gaccttgggg aagataatgt gacagagcag 2041 accaatcaca ttattattcc tagttatgca tcatggtttg attataactg tattcatgtg 2101 attgaacggc gtgctcttcc tgagttcttc aatggaaaaa acaaatccaa gactccagaa 2161 atatacttgg catatcgaaa ttttatgatt gacacgtatc gtctaaaccc ccaagagtat 2221 ttaactagca ctgcttgtcg gaggaacttg actggagatg tgtgtgctgt gatgagggtc 2281 catgcctttt tagagcagtg gggactcgtt aattaccaag ttgacccgga aagtagaccc 2341 atggcaatgg gacctcctcc tactcctcat tttaatgtat tagctgatac cccctctggg 2401 cttgtgcctc tgcatcttcg atcacctcag gttcctgctg ctcaacagat gctaaatttt 2461 cctgagaaaa acaaggaaaa accagttgat ttgcagaact ttggtctccg tactgacatt 2521 tactccaaga aaacattagc aaagagtaaa ggtgctagtg ctggaagaga atggactgaa 2581 caggagaccc ttctactcct ggaggccctg gagatgtaca aggatgattg gaacaaagtg 2641 tcggaacatg ttggaagtcg tactcaggat gaatgcatcc tccacttttt gagacttccc 2701 attgaggacc cataccttga gaattcagat gcttcccttg ggcctttggc ctaccagcct 2761 gtccccttca gtcagtcagg aaatccagtt atgagtactg ttgctttttt ggcatctgtg 2821 gtggaccctc gcgtggcatc tgctgcagca aaagcggctt tggaggagtt ttctcgggtc 2881 cgggaggagg taccactgga attggttgaa gctcatgtca agaaagtaca agaagcagca 2941 cgagcctctg ggaaagtgga tcccacctac ggtctggaga gcagctgcat tgcaggcaca 3001 gggcccgatg agccagagaa gcttgaagga gctgaagagg aaaaaatgga agccgaccct 3061 gatggtcagc agcctgaaaa ggcagaaaat aaagtggaaa atgaaacgga tgaaggtgat 3121 aaagcacaag atggagaaaa tgaaaaaaat agtgaaaagg aacaggatag tgaagtgagt 3181 gaggatacca aatcagaaga aaaggagact gaagagaaca aagaactcac tgatacatgt 3241 aaagaaagag aaagtgatac tgggaagaag aaagtagaac atgaaatttc cgaaggaaat 3301 gttgccacag ccgcagcagc tgctcttgcc tcagcggcta ccaaagccaa gcacctggct 3361 gcagtggaag aaagaaagat caagtccctg gtagctctct tggttgagac acaaatgaag 3421 aaactagaga tcaaacttcg acattttgaa gagctggaaa ctatcatgga cagagagaaa 3481 gaagctctag aacaacagag gcagcagttg cttactgaac gccaaaactt ccacatggaa 3541 cagctgaagt atgctgaatt acgagcacga cagcaaatgg aacagcagca gcatggccag 3601 aaccctcaac aggcacacca gcactcagga ggacctggcc tggccccact tggagcagca 3661 gggcaccctg gcatgatgcc tcatcaacag ccccctccct accctctgat gcaccaccag 3721 atgccaccac ctcatccacc ccagccaggt cagataccag gcccaggttc catgatgccc 3781 gggcagcaca tgccaggccg catgattccc actgttgcag ccaacatcca cccctctggg 3841 agtggcccta cccctcctgg catgccacca atgccaggaa acatcttagg accccgggta 3901 cccctgacag cacctaacgg catgtatccc cctccaccac agcagcagcc accgccacca 3961 ccacctgcag atggggtccc tccgcctcct gctcctggcc cgccagcctc agctgctcct 4021 aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa agaaaaagCT CGAGTACCCA 4081 TACGACGTAC CTGATTATGC AACTTATCCT TATGATGTAC CTGATTATGC TAGCTATCCT 4141 TATGATGTCC CAGACTACGC ATAGTGAGGG CCCGATATCT CGACAATCAA CCTCTGGATT 4201 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG 4261 GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT 4321 CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC 4381 AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA 4441 CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC 4501 TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT 4561 CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT 4621 GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC 4681 CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA 4741 CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAA CGGGGGAGGC TAACTGAAAC 4801 ACGGAAGGAG ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA 4861 AACGCACGGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT 4921 CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC 4981 CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG 5041 GCCCTGCCAT AGCAGATCTG CGCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA 5101 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 5161 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 5221 TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 5281 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 5341 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 5401 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGG 5461 GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT 5521 TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 5581 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC 5641 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 5701 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG 5761 ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA 5821 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT 5881 ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA 5941 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 6001 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC 6061 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC 6121 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC 6181 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC 6241 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 6301 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG 6361 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT 6421 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT 6481 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG 6541 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 6601 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG 6661 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG 6721 AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG 6781 ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG 6841 CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT 6901 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 6961 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC 7021 ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 7081 CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT 7141 GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC 7201 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG 7261 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT 7321 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 7381 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 7441 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 7501 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 7561 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 7621 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT 7681 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 7741 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 7801 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 7861 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG 7921 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 7981 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 8041 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 8101 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 8161 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 8221 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 8281 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 8341 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 8401 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 8461 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 8521 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 8581 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 8641 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 8701 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 8761 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 8821 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 8881 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 8941 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 9001 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 9061 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 9121 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 9181 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC 9241 TCCCGATCCC CTATGGTCGA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA 9301 GTATCTGCTC CCTGCTTGTG TGTTGGAGGT CGCTGAGTAG TGCGCGAGCA AAATTTAAGC 9361 TACAACAAGG CAAGGCTTGA CCGACAATTG CATGAAGAAT CTGCTTAGG

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.