pCMV-dPspCas13b-Alkbh5-mCherry

pCMV-dPspCas13b-Alkbh5-mCherry-FL-Neo plasmid

PVT94814

pCMV-dPspCas13b-Alkbh5-mCherry-FL-Neo plasmid Information

Plasmid name: pCMV-dPspCas13b-Alkbh5-mCherry-FL-Neo
Alias:
Gene lenght: 4518bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: chimpanzee
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pCMV-dPspCas13b-Alkbh5-mCherry-FL-Neo plasmid Description

pCMV-dPspCas13b-Alkbh5-mCherry-FL-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-dPspCas13b-Alkbh5-mCherry-FL-Neo plasmid Sequence

1 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 61 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 121 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 181 CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 241 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG ACAGGATGAG 301 GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG 361 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT 421 TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC 481 TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT 541 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG 601 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG 661 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG 721 CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG 781 ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC 841 GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA 901 TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC 961 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG 1021 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT 1081 ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC 1141 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 1201 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 1261 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 1321 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 1381 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 1441 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 1501 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 1561 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 1621 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 1681 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 1741 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 1801 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 1861 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 1921 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 1981 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 2041 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 2101 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 2161 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 2221 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 2281 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 2341 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 2401 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 2461 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 2521 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 2581 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 2641 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 2701 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 2761 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 2821 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 2881 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 2941 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 3001 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 3061 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 3121 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 3181 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 3241 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 3301 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 3361 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 3421 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 3481 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 3541 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 3601 TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT GCACTCTCAG TACAATCTGC 3661 TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG 3721 TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG 3781 AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT 3841 TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC 3901 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC 3961 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG 4021 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT 4081 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC 4141 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA 4201 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG 4261 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT 4321 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA 4381 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA 4441 GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT 4501 GGCTAGCGTT TAAACTTAAG CTTGCCACCA TGAACATCCC CGCTCTGGTG GAAAACCAGA 4561 AGAAGTACTT TGGCACCTAC AGCGTGATGG CCATGCTGAA CGCTCAGACC GTGCTGGACC 4621 ACATCCAGAA GGTGGCCGAT ATTGAGGGCG AGCAGAACGA GAACAACGAG AATCTGTGGT 4681 TTCACCCCGT GATGAGCCAC CTGTACAACG CCAAGAACGG CTACGACAAG CAGCCCGAGA 4741 AAACCATGTT CATCATCGAG CGGCTGCAGA GCTACTTCCC ATTCCTGAAG ATCATGGCCG 4801 AGAACCAGAG AGAGTACAGC AACGGCAAGT ACAAGCAGAA CCGCGTGGAA GTGAACAGCA 4861 ACGACATCTT CGAGGTGCTG AAGCGCGCCT TCGGCGTGCT GAAGATGTAC AGGGACCTGA 4921 CCAACGCATA CAAGACCTAC GAGGAAAAGC TGAACGACGG CTGCGAGTTC CTGACCAGCA 4981 CAGAGCAACC TCTGAGCGGC ATGATCAACA ACTACTACAC AGTGGCCCTG CGGAACATGA 5041 ACGAGAGATA CGGCTACAAG ACAGAGGACC TGGCCTTCAT CCAGGACAAG CGGTTCAAGT 5101 TCGTGAAGGA CGCCTACGGC AAGAAAAAGT CCCAAGTGAA TACCGGATTC TTCCTGAGCC 5161 TGCAGGACTA CAACGGCGAC ACACAGAAGA AGCTGCACCT GAGCGGAGTG GGAATCGCCC 5221 TGCTGATCTG CCTGTTCCTG GACAAGCAGT ACATCAACAT CTTTCTGAGC AGGCTGCCCA 5281 TCTTCTCCAG CTACAATGCC CAGAGCGAGG AACGGCGGAT CATCATCAGA TCCTTCGGCA 5341 TCAACAGCAT CAAGCTGCCC AAGGACCGGA TCCACAGCGA GAAGTCCAAC AAGAGCGTGG 5401 CCATGGATAT GCTCAACGAA GTGAAGCGGT GCCCCGACGA GCTGTTCACA ACACTGTCTG 5461 CCGAGAAGCA GTCCCGGTTC AGAATCATCA GCGACGACCA CAATGAAGTG CTGATGAAGC 5521 GGAGCAGCGA CAGATTCGTG CCTCTGCTGC TGCAGTATAT CGATTACGGC AAGCTGTTCG 5581 ACCACATCAG GTTCCACGTG AACATGGGCA AGCTGAGATA CCTGCTGAAG GCCGACAAGA 5641 CCTGCATCGA CGGCCAGACC AGAGTCAGAG TGATCGAGCA GCCCCTGAAC GGCTTCGGCA 5701 GACTGGAAGA GGCCGAGACA ATGCGGAAGC AAGAGAACGG CACCTTCGGC AACAGCGGCA 5761 TCCGGATCAG AGACTTCGAG AACATGAAGC GGGACGACGC CAATCCTGCC AACTATCCCT 5821 ACATCGTGGA CACCTACACA CACTACATCC TGGAAAACAA CAAGGTCGAG ATGTTTATCA 5881 ACGACAAAGA GGACAGCGCC CCACTGCTGC CCGTGATCGA GGATGATAGA TACGTGGTCA 5941 AGACAATCCC CAGCTGCCGG ATGAGCACCC TGGAAATTCC AGCCATGGCC TTCCACATGT 6001 TTCTGTTCGG CAGCAAGAAA ACCGAGAAGC TGATCGTGGA CGTGCACAAC CGGTACAAGA 6061 GACTGTTCCA GGCCATGCAG AAAGAAGAAG TGACCGCCGA GAATATCGCC AGCTTCGGAA 6121 TCGCCGAGAG CGACCTGCCT CAGAAGATCC TGGATCTGAT CAGCGGCAAT GCCCACGGCA 6181 AGGATGTGGA CGCCTTCATC AGACTGACCG TGGACGACAT GCTGACCGAC ACCGAGCGGA 6241 GAATCAAGAG ATTCAAGGAC GACCGGAAGT CCATTCGGAG CGCCGACAAC AAGATGGGAA 6301 AGAGAGGCTT CAAGCAGATC TCCACAGGCA AGCTGGCCGA CTTCCTGGCC AAGGACATCG 6361 TGCTGTTTCA GCCCAGCGTG AACGATGGCG AGAACAAGAT CACCGGCCTG AACTACCGGA 6421 TCATGCAGAG CGCCATTGCC GTGTACGATA GCGGCGACGA TTACGAGGCC AAGCAGCAGT 6481 TCAAGCTGAT GTTCGAGAAG GCCCGGCTGA TCGGCAAGGG CACAACAGAG CCTCATCCAT 6541 TTCTGTACAA GGTGTTCGCC CGCAGCATCC CCGCCAATGC CGTCGAGTTC TACGAGCGCT 6601 ACCTGATCGA GCGGAAGTTC TACCTGACCG GCCTGTCCAA CGAGATCAAG AAAGGCAACA 6661 GAGTGGATGT GCCCTTCATC CGGCGGGACC AGAACAAGTG GAAAACACCC GCCATGAAGA 6721 CCCTGGGCAG AATCTACAGC GAGGATCTGC CCGTGGAACT GCCCAGACAG ATGTTCGACA 6781 ATGAGATCAA GTCCCACCTG AAGTCCCTGC CACAGATGGA AGGCATCGAC TTCAACAATG 6841 CCAACGTGAC CTATCTGATC GCCGAGTACA TGAAGAGAGT GCTGGACGAC GACTTCCAGA 6901 CCTTCTACCA GTGGAACCGC AACTACCGGT ACATGGACAT GCTTAAGGGC GAGTACGACA 6961 GAAAGGGCTC CCTGCAGCAC TGCTTCACCA GCGTGGAAGA GAGAGAAGGC CTCTGGAAAG 7021 AGCGGGCCTC CAGAACAGAG CGGTACAGAA AGCAGGCCAG CAACAAGATC CGCAGCAACC 7081 GGCAGATGAG AAACGCCAGC AGCGAAGAGA TCGAGACAAT CCTGGATAAG CGGCTGAGCA 7141 ACAGCCGGAA CGAGTACCAG AAAAGCGAGA AAGTGATCCG GCGCTACAGA GTGCAGGATG 7201 CCCTGCTGTT TCTGCTGGCC AAAAAGACCC TGACCGAACT GGCCGATTTC GACGGCGAGA 7261 GGTTCAAACT GAAAGAAATC ATGCCCGACG CCGAGAAGGG AATCCTGAGC GAGATCATGC 7321 CCATGAGCTT CACCTTCGAG AAAGGCGGCA AGAAGTACAC CATCACCAGC GAGGGCATGA 7381 AGCTGAAGAA CTACGGCGAC TTCTTTGTGC TGGCTAGCGA CAAGAGGATC GGCAACCTGC 7441 TGGAACTCGT GGGCAGCGAC ATCGTGTCCA AAGAGGATAT CATGGAAGAG TTCAACAAAT 7501 ACGACCAGTG CAGGCCCGAG ATCAGCTCCA TCGTGTTCAA CCTGGAAAAG TGGGCCTTCG 7561 ACACATACCC CGAGCTGTCT GCCAGAGTGG ACCGGGAAGA GAAGGTGGAC TTCAAGAGCA 7621 TCCTGAAAAT CCTGCTGAAC AACAAGAACA TCAACAAAGA GCAGAGCGAC ATCCTGCGGA 7681 AGATCCGGAA CGCCTTCGAT GCAAACAATT ACCCCGACAA AGGCGTGGTG GAAATCAAGG 7741 CCCTGCCTGA GATCGCCATG AGCATCAAGA AGGCCTTTGG GGAGTACGCC ATCATGAAGG 7801 GAAGCCTGCA GCTGCCTCCA CTTGAAAGAC TGACACTGGG ATCCGGAGGA GGTGGAAGCG 7861 GTACCATGGC GGCCGCCAGC GGCTACACGG ACCTGCGTGA GAAGCTCAAG TCCATGACGT 7921 CCCGGGACAA CTATAAGGCG GGCAGCCGGG AGGCCGCCGC CGCTGCCGCA GCCGCCGTAG 7981 CCGCCGCAGC CGCAGCCGCC GCTGCCGCCG AACCTTACCC TGTGTCCGGG GCCAAGCGCA 8041 AGTATCAGGA GGACTCGGAC CCCGAGCGCA GCGACTATGA GGAGCAGCAG CTGCAGAAGG 8101 AGGAGGAGGC GCGCAAGGTG AAGAGCGGCA TCCGCCAGAT GCGCCTCTTC AGCCAGGACG 8161 AGTGCGCCAA GATCGAGGCC CGCATTGACG AGGTGGTGTC CCGCGCTGAG AAGGGCCTGT 8221 ACAACGAGCA CACGGTGGAC CGGGCCCCAC TGCGCAACAA GTACTTCTTC GGCGAAGGCT 8281 ACACTTACGG CGCCCAGCTG CAGAAGCGCG GGCCCGGCCA GGAGCGCCTC TACCCGCCGG 8341 GCGACGTGGA CGAGATCCCC GAGTGGGTGC ACCAGCTGGT GATCCAAAAG CTGGTGGAGC 8401 ACCGCGTCAT CCCCGAGGGC TTCGTCAACA GCGCCGTCAT CAACGACTAC CAGCCCGGCG 8461 GCTGCATCGT GTCTCACGTG GACCCCATCC ACATCTTCGA GCGCCCCATC GTGTCCGTGT 8521 CCTTCTTTAG CGACTCTGCG CTGTGCTTCG GCTGCAAGTT CCAGTTCAAG CCTATTCGGG 8581 TGTCGGAACC AGTGCTTTCC CTGCCGGTGC GCAGGGGAAG CGTGACTGTG CTCAGTGGAT 8641 ATGCTGCTGA TGAAATCACT CACTGCATAC GGCCTCAGGA CATCAAGGAG CGCCGAGCAG 8701 TCATCATCCT CAGGAAGACA AGATTAGATG CACCCCGGTT GGAAACAAAG TCCCTGAGCA 8761 GCTCCGTGTT ACCACCCAGC TATGCTTCAG ATCGCCTGTC AGGAAACAAC AGGGACCCTG 8821 CTCTGAAACC CAAGCGGTCC CACCGCAAGG CAGACCCTGA TGCTGCCCAC AGGCCACGGA 8881 TCCTGGAGAT GGACAAGGAA GAGAACCGGC GCTCGGTGCT GCTGCCCACA CACCGGCGGA 8941 GGGGTAGCTT CAGCTCTGAG AACTACTGGC GCAAGTCATA CGAGTCCTCA GAGGACTGCT 9001 CTGAGGCAGC AGGCAGCCCT GCCCGAAAGG TGAAGATGCG GCGGCACGAA TTCggtggag 9061 gcggatcagg aggcggtgga tctatggtga gcaagggcga ggaggataac atggccatca 9121 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 9181 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 9241 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 9301 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 9361 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 9421 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 9481 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 9541 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 9601 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 9661 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 9721 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 9781 agctgtacaa gtagCTCGAG TCTAGAGGGC CCGTTTAAAC CCGCTGATCA GCCTCGACTG 9841 TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG 9901 AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA 9961 GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG 10021 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA 10081 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 10141 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 10201 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 10261 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 10321 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 10381 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 10441 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 10501 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT 10561 AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 10621 TTAGTCAGCA ACCA

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.