pLV2-CMV-3×FLAG-JUN-Puro plasm

pLV2-CMV-3×FLAG-JUN-Puro plasmid

PVT94755

pLV2-CMV-3×FLAG-JUN-Puro plasmid Information

Plasmid name: pLV2-CMV-3×FLAG-JUN(human)-Puro
Alias: NM_002228.4;AP-1; AP1; c-Jun; cJUN; p39;
Gene lenght: 993bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-3×FLAG-JUN-Puro plasmid Description

pLV2-CMV-3×FLAG-JUN-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-3×FLAG-JUN-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgaG 2821 CCACCATGGA CTACAAAGAC CATGACGGTG ATTATAAAGA TCATGACATC GACTACAAGG 2881 ATGACGATGA CAAGgaattc atgactgcaa agatggaaac gaccttctat gacgatgccc 2941 tcaacgcctc gttcctcccg tccgagagcg gaccttatgg ctacagtaac cccaagatcc 3001 tgaaacagag catgaccctg aacctggccg acccagtggg gagcctgaag ccgcacctcc 3061 gcgccaagaa ctcggacctc ctcacctcgc ccgacgtggg gctgctcaag ctggcgtcgc 3121 ccgagctgga gcgcctgata atccagtcca gcaacgggca catcaccacc acgccgaccc 3181 ccacccagtt cctgtgcccc aagaacgtga cagatgagca ggagggcttc gccgagggct 3241 tcgtgcgcgc cctggccgaa ctgcacagcc agaacacgct gcccagcgtc acgtcggcgg 3301 cgcagccggt caacggggca ggcatggtgg ctcccgcggt agcctcggtg gcagggggca 3361 gcggcagcgg cggcttcagc gccagcctgc acagcgagcc gccggtctac gcaaacctca 3421 gcaacttcaa cccaggcgcg ctgagcagcg gcggcggggc gccctcctac ggcgcggccg 3481 gcctggcctt tcccgcgcaa ccccagcagc agcagcagcc gccgcaccac ctgccccagc 3541 agatgcccgt gcagcacccg cggctgcagg ccctgaagga ggagcctcag acagtgcccg 3601 agatgcccgg cgagacaccg cccctgtccc ccatcgacat ggagtcccag gagcggatca 3661 aggcggagag gaagcgcatg aggaaccgca tcgctgcctc caagtgccga aaaaggaagc 3721 tggagagaat cgcccggctg gaggaaaaag tgaaaacctt gaaagctcag aactcggagc 3781 tggcgtccac ggccaacatg ctcagggaac aggtggcaca gcttaaacag aaagtcatga 3841 accacgttaa cagtgggtgc caactcatgc taacgcagca gttgcaaaca ttttgaggat 3901 cccgcgactc tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag 3961 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca 4021 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc 4081 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca 4141 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg 4201 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt 4261 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg 4321 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca 4381 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct 4441 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc 4501 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc 4561 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 4621 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 4681 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 4741 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 4801 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 4861 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 4921 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 4981 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 5041 ctgaccgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 5101 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 5161 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 5221 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 5281 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 5341 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 5401 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 5461 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 5521 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 5581 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 5641 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag 5701 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 5761 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 5821 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag 5881 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa 5941 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt 6001 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca 6061 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga 6121 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg 6181 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct 6241 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct 6301 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 6361 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 6421 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 6481 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 6541 tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc 6601 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 6661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 6721 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 6781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 6841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 7021 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 7081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 7141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 7201 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 7261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 7321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 7381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 7441 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 7501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 7561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 7621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 7681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 7741 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 7801 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 7861 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 7921 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 7981 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 8041 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 8101 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 8161 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 8221 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 8281 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 8341 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 8401 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 8461 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 8521 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 8581 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 8641 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 8701 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8761 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa 8821 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 8881 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc 8941 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg 9001 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat 9061 ttgttaaata tgtactacaa acttagtagt ttttaaagaa attgtatttg ttaaatatgt 9121 actacaaact tagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.