pLV3-CMV-Paf1-3×FLAG-CopGFP-Pu

pLV3-CMV-Paf1-3×FLAG-CopGFP-Puro plasmid

PVT94733

pLV3-CMV-Paf1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Paf1(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_019458.3;5730511K23Rik; AA675045
Gene lenght: 1605bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Paf1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Paf1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Paf1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg cgcccaccat ccagacccag 2341 gcccagaggg aagatggcca caggcccaat tcccaccgga ccttgcctga gaggtctgga 2401 gtagtctgcc gagtcaagta ctgcaatagc cttcctgaca tcccatttga ccccaagttc 2461 atcacctacc ccttcgacca gaacaggttt gttcagtaca aagcaacctc cttggagaaa 2521 cagcacaaac atgacctcct gactgagcca gatctggggg tcaccattga tctcatcaac 2581 cctgacacct accgcattga tcccaatgtt cttctggatc cggctgatga gaagcttcta 2641 gaagaggaga ttcaggctcc caccagctct aagagatccc agcagcatgc aaaggtggta 2701 ccgtggatgc gtaagacaga gtatatctcc actgagttca acagatatgg catctccaat 2761 gagaagcctg aagtcaagat tggggtttct gtgaaacagc agttcacaga ggaagaaata 2821 tacaaagaca gggacagcca aatcacagcc attgaaaaga cttttgaaga tgcccagaaa 2881 tcgatctccc agcattacag caagccccga gtgacaccag tggaggtcat gcctgtcttc 2941 ccagatttta agatgtggat aaacccatgt gctcaggtca tttttgactc agacccagcc 3001 cccaaggaca caagtggtgc agctgcatta gagatgatgt ctcaggccat gatcaggggc 3061 atgatggatg aggaagggaa ccagtttgtg gcttattttc tgcctgtgga agaaacacta 3121 aagaaacgaa agcgggacca agaggaagag atggactatg caccagatga tgtgtatgac 3181 tataagattg ctcgggaata caactggaat gtgaagaaca aggccagtaa gggctatgag 3241 gagaactatt tcttcatctt cagagagggt gacggagttt actacaatga gctggagacc 3301 agggtccgtc taagtaagcg ccgggccaag gctggggttc agtcaggtac caatgccttg 3361 cttgtcgtca aacaccgtga catgaatgag aaggaattag aagcccagga ggcacgaaag 3421 gcccagttgg aaaaccacga accagaggaa gaggaggagg aagagatgga ggctgaagag 3481 aaagaagctg ggggctcaga tgaggaacag gagaagggta gcagcagtga gaaagaaggc 3541 agcgaggatg aacactctgg aagtgagagt gaccgagaag agggtgacag agatgaggca 3601 agtgacaaga gtggcagcgg tgaggatgaa agcagtgagg atgaagcacg tgctgcccgg 3661 gacaaagagg agatttttgg tagtgatgct gattcagaag atgatgctga ctctgatgat 3721 gaggacagag ggcaggccca caggggcagt gacaatgact cggacagcgg cagtgatggt 3781 ggtggccaga ggagccgcag ccagagcagg agtcgtagcc ggagtgccag ccccttcccc 3841 agtggcagtg agcactcagc tcaggaagat ggcagtgaag cagcagcctc tgattccagt 3901 gaggctgaca gtgacagtga tggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3961 CATGACATCG ACTACAAGGA TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc 4021 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 4081 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 4141 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 4201 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga 4261 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg 4321 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag 4381 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct 4441 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt 4501 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc 4561 accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc 4621 accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc 4681 cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg 4741 agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac 4801 cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag 4861 gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc 4921 gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc 4981 atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc 5041 agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc 5101 cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc 5161 gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag 5221 cacgccttca agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc 5281 gtggacggca ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta 5341 acatgcggtg acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc 5401 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 5461 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 5521 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 5581 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 5641 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 5701 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 5761 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 5821 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 5881 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 5941 acctggtgca tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt 6001 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 6061 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 6121 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 6181 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 6241 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 6301 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 6361 tcggggaaAT CATCGTCCTT TCCTtggctg ctcgcctgtg ttgccacctg gattctgcgc 6421 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 6481 ctgctgccgg ctctgcggcc tcttccgcgt ctTcgccttc gccctcagac gagtcggatc 6541 tccctttggG ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6601 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6661 aaGataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6721 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6781 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 6841 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 6901 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 6961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 7081 aTCCCGCCCC TAACTCCGCC CAtcccgccc ctaactccgc ccagttccgc ccattctccg 7141 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 7201 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agaccaaatt 7261 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 7321 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 7381 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 7441 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 7501 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 7561 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 7621 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 7681 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 7741 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 7801 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 7861 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 7921 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 7981 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 8041 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 8101 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 8161 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 8221 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 8281 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 8341 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 8401 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 8461 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 8521 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 8581 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 8641 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 8701 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 8761 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 8821 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 8881 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 8941 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 9001 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 9061 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 9121 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 9181 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 9241 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 9301 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 9361 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 9421 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 9481 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 9541 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 9601 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt 9661 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg 9721 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 9781 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 9841 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.