pCMV-3×FLAG-RPS5-Neo plasmid

pCMV-3×FLAG-RPS5-Neo plasmid

PVT94687

pCMV-3×FLAG-RPS5-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-RPS5(human)-Neo
Alias: NM_001009.4;S5; uS7
Gene lenght: 615bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-RPS5-Neo plasmid Description

pCMV-3×FLAG-RPS5-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-RPS5-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atgaccgagt gggagacagc agcaccagcg 1021 gtggcagaga ccccagacat caagctcttt gggaagtgga gcaccgatga tgtgcagatc 1081 aatgacattt ccctgcagga ttacattgca gtgaaggaga agtatgccaa gtacctgcct 1141 cacagtgcag ggcggtatgc cgccaaacgc ttccgcaaag ctcagtgtcc cattgtggag 1201 cgcctcacta actccatgat gatgcacggc cgcaacaacg gcaagaagct catgactgtg 1261 cgcatcgtca agcatgcctt cgagatcata cacctgctca caggcgagaa ccctctgcag 1321 gtcctggtga acgccatcat caacagtggt ccccgggagg actccacacg cattgggcgc 1381 gccgggactg tgagacgaca ggctgtggat gtgtcccccc tgcgccgtgt gaaccaggcc 1441 atctggctgc tgtgcacagg cgctcgtgag gctgccttcc ggaacattaa gaccattgct 1501 gagtgcctgg cagatgagct catcaatgct gccaagggct cctcgaactc ctatgccatt 1561 aagaagaagg acgagctgga gcgtgtggcc aagtccaacc gctgagaatt ctgcagatat 1621 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg 1681 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 1741 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 1801 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 1861 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 1921 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 1981 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2041 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2101 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 2161 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 2221 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 2281 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 2341 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 2401 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 2461 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2521 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 2581 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 2641 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 2701 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 2761 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 2821 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 2881 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 2941 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3001 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3061 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3121 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 3181 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 3241 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 3301 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 3361 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 3421 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 3481 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 3541 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 3601 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 3661 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 3721 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 3781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 3841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 3901 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 3961 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4021 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4081 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4141 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 4201 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 4261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 4801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4861 tagcggtGGT ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6001 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6061 tccccgaaaa gtgccacctg acgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.