pBiFC-CMV-Kif24-VN173 plasmid

pBiFC-CMV-Kif24-VN173 plasmid

PVT94673

pBiFC-CMV-Kif24-VN173 plasmid Information

Plasmid name: pBiFC-CMV-Kif24(mouse)-VN173
Alias: NM_024241.3;4933425J19Rik; 9430029L23Rik;
Gene lenght: 4068bp
Host: Mammalian cells
Use(s): Bimolecular fluorescence
Fragment type: BIFC
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pBiFC-CMV-Kif24-VN173 plasmid Description

pBiFC-CMV-Kif24-VN173 This is a plasmid suitable for bimolecular fluorescence in mammalian cells.

pBiFC-CMV-Kif24-VN173 plasmid Sequence

1 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 61 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 121 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTGATCT ATACATTGAA 181 TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA GCATAAATCA ATATTGGCTA 241 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC 301 AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG 361 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 421 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 481 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 541 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA 601 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG 661 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC 721 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 781 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC 841 CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC 901 TCGTTTAGTG AACCGTCAGA ATTGATCTAC CATGGACTAC AAAGACGATG ACGACAAGCT 961 Tatggcatcc tggttatatg agtgtctttg tgaggctgag cttgcacagt attatcctca 1021 ttttactgcg cttggccttc aaaaaataga tgaattagcc aaggttacaa tgaaggacta 1081 ctccagattg ggagtccatg acatgaacga ccgcaaacgt ctctttcagc ttatcaaaat 1141 cattaagatt atgcaggagg aagataaagc cctcggtatc ccagagcatc ctcttcaggc 1201 aagcagcctg tacaccaagc ctcgggaatt cagatctggg ccccgaagac aactgcattt 1261 tgattctcct tctgccagca aagataaaat ggccaacaat gaaacaggca gtttatccaa 1321 cttctctgtg gatgagcaga agtccactta cctgaaagtg ctggagcaca tgctaccaga 1381 tgattcccag tgccagacaa aaataagagc cccggatgcc tctgctgctg atgcttccat 1441 gcaaacggaa accaacgctc cgctcttttc atcaaattac ttctctccac aactgggaaa 1501 ttgtgatatt cccgttattc aaagagtctc tcatgtgtca gggtataact atggaattcc 1561 tcattcttgt gtcaggcaga tcacctcaga gaatccttgg actgaaatgg agaaaatcag 1621 agtttgtgtt cgaaaacggc ctttaggtgt aagggaagta cgtcgtggag aagttaatgt 1681 tattactgta gaagataaag aaactctgct cgtacatgag aaaaaagaag cagttgacct 1741 tactcaatat attcttcagc atgtttttta ttttgatgaa gtctttggtg aggcatgcag 1801 caatcaagat gtatacctga agactgctca ccccctcatt cagcatattt tcaacggagg 1861 aagtgccact tgctttgcat atggacaaac aggtgcgggg aaaacctata ccatgatagg 1921 aactcatcaa aacccaggat tgtatgctct ggctgccaaa gatatcttca ggcagctgaa 1981 agtatcccag tcaagaagga atctctttgt gtggatcagc ttctatgaga tctactgtgg 2041 acaactttat gacctcctaa atagaagaaa acggctcttt gcaagagaag atagcaagca 2101 tgtggtacag atagctggcc ttcgagagct ccaagtggac agtgtggagc tactcctaca 2161 ggtgatctta aagggcagca aggagcgcag cacaggggcc actggtgtca acgcagactc 2221 ctcccgttcc catgctatca tccaaattca gatcaaagat tcagccaaga ggacatttgg 2281 caggatctct ttcattgact tggctgggag tgagagagca gcagatgcca gggactcaga 2341 cagacagaca aagatggaag gcgcggagat caaccagagt cttctggctc tgaaagaatg 2401 tatccgagca ctggaccagg aacacaccca cacgcctttc aggcagagca aactgactca 2461 ggtcctgaag gactctttca ttggcaatgc caaaacttgc atgatcgcca acatctcccc 2521 aagccacata gccacagagc atacattgaa taccttgcgc tatgctgacc gggtgaagga 2581 actaaagaaa ggcgttaagt gttgtgcttc agctaccagt cagaatcaga catctgcaaa 2641 cgcctctcca aaacgaattc agagctcccc tgtcaccctc cccggggaca agtgctcccc 2701 caaaaaagtt aagctgggac ttcagcagtc acttactgtg gccccaggcc ccacaaaagt 2761 taaagcccat cctttggcca gccatgttcc caacgtcccc tttacctctg gacctaaaac 2821 ccctggtaaa aaaagtagtt cccgagggag tcccactcca gagtgggaca tgaaggctag 2881 ccctcgcaaa ggaactacga gatctggcca ttcgataaaa aagggagcag agtcggcacc 2941 actgtgctct gagaaaagtc aaattggcag caagattgct gttgggtggg agggcagggc 3001 ctcagaccca ggagaaggtc tgctgcgtgt caggctgccc acgagaggga agaaggtgca 3061 gccagtccag ccagtgcaga agcagctcct gtcccgacct cggctccttg ctaacagcca 3121 ccacttagaa gccactcagg acagcaaggt gggcacacct gccgggcttg cccccgaagc 3181 ctggacaaac cccattctac agcaaaagga aagggaggag catttgcgat tctaccacca 3241 gcagttccag cagccaccgc tcctcaagca gaagctaaat taccaacccc tgcagaggct 3301 tttatgccag cacaggcctt cagaaggtcg gctccagagt gagactggct ttcccctcca 3361 ctctaaccct gagaaccgtg atggagccca ggctgaggac cttgatgaca gtgacttcag 3421 tgaggattct ttctcgcacg gctccagtca gccggccatg aaacagggga gcacagcctt 3481 ggaaaggagc ggaagttcct tcttccttca tcaggacagg gagcacagcc ctgaggagca 3541 ggcagcagaa aggcagcaat gtttgctttt cagctctgag acggatggca gtaagaagcg 3601 gccagctgac agctgggtgt attccaggga ccccatcatc agccacagaa gaggagcact 3661 cagtcaaagc cacagcccaa gtatggtatg cccagactgg agcaaggagg aagactctgc 3721 ctcctcagga ccttctccta aggacaaccg ggcccagaaa ccttcctctt cacaggtaga 3781 ttttgtgcat caccaaaaac cgggtgaagc tcaagtctct gacatcagac tggaggcctt 3841 cacaagtgag gttcctgagc aagctgaggg cagcttgtca tccccatccc cggaaaatgg 3901 gctgtctttt ccactgtccc atgtggctgt ttctggatcc ccagaccaaa gagacagagt 3961 ctgcacacca ttaagagaag tcagtgaaaa cagagtgact cacactccag gaagagtgaa 4021 cagcagcact cctttccaag aggactctgg agagcagata cagatgtgtt ctgcaaatgc 4081 ttctggactc atggctcccc tcactatgtc cctcctggag accccctgtc atgaggacct 4141 ttcgtccttg gagcaaattg cccaggatgg agctggctat ggtttcatgg ctgagattgt 4201 aggagggcca gcagcaggac acacagtccc atcttatgat caagaggctg ctttgccagt 4261 gtcttcagct actgagtgcc tatggctatc ttcatctccc cctgacaatc ggcctagtgg 4321 tgatcttcca gctctgtccc cgtcacccat ccaccagcat tcacctgaca aattgcccgg 4381 tagagaggcc taccagacca gaaggccaat actcttgcct gagaatcata tgggtagtaa 4441 gctatatgat gaccgtgctg aagagactga actggggggc tccctcacat tccccagaaa 4501 gccgtcctcc aacatacatg ccggagtacc ctattctaca cctttcctca cctcatgtac 4561 aggaagtagt aatggagttg gccgtccctg ggcccaggag agaaaacatc ctacaggggt 4621 tagctgccag gagcttgttt cctctacaga ttccaacaag cctcattaca acgaggacat 4681 cgcatggctc aggcacaggc caatctcaag gtgcttagat tcagactctc ctgtggtccc 4741 cagctgttct tccaaggcct tgaggacata ttgtccactt acaccggagc aggcacagca 4801 ggtgattatc cgtgcacaca aagaacagct ggatgaaatg gctgagctgg acttgaagga 4861 ggagacctta atgacccaga tggattctaa tgattttgaa gattttgtga cccagctgga 4921 tgaaatcatg gctttgaagt ccaggtgcat ccaaagtctg agaagccagc tacagctcta 4981 cctcaccagc cacaggcccg ctgcagcccc cgaaagaact gtggtgtctT CTAGAAGATC 5041 CATCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT 5101 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA 5161 TGCCACCTAC GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC 5221 CTGGCCCACC CTCGTGACCA CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA 5281 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG 5341 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG 5401 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT 5461 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA 5521 GCAGAAGAAC GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGT AGGGATCCCG 5581 GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG 5641 TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT 5701 TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA 5761 CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC 5821 TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT 5881 TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG 5941 GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT 6001 GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA 6061 TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG 6121 CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA 6181 CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTG GCTGTGGAAT GTGTGTCAGT 6241 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 6301 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA 6361 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC 6421 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG 6481 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG 6541 GAGGCCTAGG CTTTTGCAAA AAGCTCCTCG AGGAACTGAA AAACCAGAAA GTTAATTCCC 6601 TATAGTGAGT CGTATTAAAT TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA 6661 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 6721 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 6781 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 6841 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 6901 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 6961 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 7021 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 7081 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 7141 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 7201 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 7261 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 7321 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 7381 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 7441 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 7501 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 7561 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 7621 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 7681 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 7741 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 7801 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 7861 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 7921 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 7981 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 8041 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 8101 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 8161 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 8221 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 8281 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 8341 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 8401 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 8461 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 8521 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 8581 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 8641 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 8701 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 8761 ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 8821 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 8881 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 8941 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 9001 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 9061 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 9121 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA 9181 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.