pLV3-CMV-CD19-Luc-Hygro plasmi

pLV3-CMV-CD19-Luc-Hygro plasmid

PVT94570

pLV3-CMV-CD19-Luc-Hygro plasmid Information

Plasmid name: pLV3-CMV-CD19(human)-Luc-Hygro
Alias: NM_001770.6;CD19/B4/CVID3
Gene lenght: 1668bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: IRES-F1
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CD19-Luc-Hygro plasmid Description

pLV3-CMV-CD19-Luc-Hygro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-CD19-Luc-Hygro plasmid Sequence

1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc 4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagagctag 4741 cgaattcgcc accatgccac ctcctcgcct cctcttcttc ctcctcttcc tcacccccat 4801 ggaagtcagg cccgaggaac ctctagtggt gaaggtggaa gagggagata acgctgtgct 4861 gcagtgcctc aaggggacct cagatggccc cactcagcag ctgacctggt ctcgggagtc 4921 cccgcttaaa cccttcttaa aactcagcct ggggctgcca ggcctgggaa tccacatgag 4981 gcccctggcc atctggcttt tcatcttcaa cgtctctcaa cagatggggg gcttctacct 5041 gtgccagccg gggcccccct ctgagaaggc ctggcagcct ggctggacag tcaatgtgga 5101 gggcagcggg gagctgttcc ggtggaatgt ttcggaccta ggtggcctgg gctgtggcct 5161 gaagaacagg tcctcagagg gccccagctc cccttccggg aagctcatga gccccaagct 5221 gtatgtgtgg gccaaagacc gccctgagat ctgggaggga gagcctccgt gtctcccacc 5281 gagggacagc ctgaaccaga gcctcagcca ggacctcacc atggcccctg gctccacact 5341 ctggctgtcc tgtggggtac cccctgactc tgtgtccagg ggccccctct cctggaccca 5401 tgtgcacccc aaggggccta agtcattgct gagcctagag ctgaaggacg atcgcccggc 5461 cagagatatg tgggtaatgg agacgggtct gttgttgccc cgggccacag ctcaagacgc 5521 tggaaagtat tattgtcacc gtggcaacct gaccatgtca ttccacctgg agatcactgc 5581 tcggccagta ctatggcact ggctgctgag gactggtggc tggaaggtct cagctgtgac 5641 tttggcttat ctgatcttct gcctgtgttc ccttgtgggc attcttcatc ttcaaagagc 5701 cctggtcctg aggaggaaaa gaaagcgaat gactgacccc accaggagat tcttcaaagt 5761 gacgcctccc ccaggaagcg ggccccagaa ccagtacggg aacgtgctgt ctctccccac 5821 acccacctca ggcctcggac gcgcccagcg ttgggccgca ggcctggggg gcactgcccc 5881 gtcttatgga aacccgagca gcgacgtcca ggcggatgga gccttggggt cccggagccc 5941 gccgggagtg ggcccagaag aagaggaagg ggagggctat gaggaacctg acagtgagga 6001 ggactccgag ttctatgaga acgactccaa ccttgggcag gaccagctct cccaggatgg 6061 cagcggctac gagaaccctg aggatgagcc cctgggtcct gaggatgaag actccttctc 6121 caacgctgag tcttatgaga acgaggatga agagctgacc cagccggtcg ccaggacaat 6181 ggacttcctg agccctcatg ggtcagcctg ggaccccagc cgggaagcaa cctccctggg 6241 gtcccagtcc tatgaggata tgagaggaat cctgtatgca gccccccagc tccgctccat 6301 tcggggccag cctggaccca atcatgagga agatgcagac tcttatgaga acatggataa 6361 tcccgatggg ccagacccag cctggggagg agggggccgc atgggcacct ggagcaccag 6421 gGAATTCGGA GGAGGGGGAA GCgGCGGCGG AGGGAGTatg gaagacgcca aaaacataaa 6481 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa 6541 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga 6601 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa 6661 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt 6721 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat 6781 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt 6841 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa 6901 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt 6961 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag 7021 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt 7081 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg 7141 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg 7201 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt 7261 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt 7321 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa 7381 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc 7441 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc 7501 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt 7561 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg 7621 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac 7681 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga 7741 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca 7801 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg 7861 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga 7921 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac 7981 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg 8041 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat 8101 cgccgtgtaa gGATCCGCCC CTCTCCCTCC CCCCCCCCTA ACGTTACTGG CCGAAGCCGC 8161 TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT CCACCATATT GCCGTCTTTT 8221 GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA CGAGCATTCC TAGGGGTCTT 8281 TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG TGAAGGAAGC AGTTCCTCTG 8341 GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT GCAGGCAGCG GAACCCCCCA 8401 CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT AAGATACACC TGCAAAGGCG 8461 GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGG AAAGAGTCAA ATGGCTCTCC 8521 TCAAGCGTAT TCAACAAGGG GCTGAAGGAT GCCCAGAAGG TACCCCATTG TATGGGATCT 8581 GATCTGGGGC CTCGGTGCAC ATGCTTTACA TGTGTTTAGT CGAGGTTAAA AAAACGTCTA 8641 GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA CACGATGATA AacgcgtACC 8701 ATGAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC 8761 AGCGTCTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT 8821 GTAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT 8881 CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT 8941 GGGGAATTCA GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG 9001 CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT 9061 GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA 9121 ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT 9181 CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG 9241 CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC 9301 TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG 9361 ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT 9421 TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG 9481 CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC 9541 GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA 9601 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC 9661 TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG 9721 GAATAGagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 9781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 9841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 9901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 9961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 10021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 10081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 10141 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 10201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 10261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 10321 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 10381 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa 10441 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 10501 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 10561 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 10621 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 10681 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 10741 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 10801 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 10861 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 10921 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 10981 ttggaggcct aggcttttgc gtcgagacgt acccaattcg ccctatagtg agtcgtatta 11041 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 11101 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 11161 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcg acgcgccctg 11221 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc 11281 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg 11341 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg 11401 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg 11461 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt 11521 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt 11581 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 11641 taacaaaata ttaacgttta caatttcc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.