pAAV-Camk2-PRKAR2A-3×FLAG plas

pAAV-Camk2-PRKAR2A-3×FLAG plasmid

PVT94504

pAAV-Camk2-PRKAR2A-3×FLAG plasmid Information

Plasmid name: pAAV-Camk2-PRKAR2A(human)-3×FLAG
Alias: NM_004157.4;PKR2; PRKAR2
Gene lenght: 1212bp
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CaMKII
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pAAV-Camk2-PRKAR2A-3×FLAG plasmid Description

pAAV-Camk2-PRKAR2A-3×FLAG is a plasmid suitable for use in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-Camk2-PRKAR2A-3×FLAG plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt 181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt 241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt 301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact 361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca 421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc 481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat 541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc 601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat 661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct 721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc 781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga 841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac 901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc 961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa 1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat 1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc 1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa 1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt 1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga 1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca 1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg 1441 ggggcagctc tagagcggta ccGGATCCat gagccacatc cagatcccgc cggggctcac 1501 ggagctgctg cagggctaca cggtggaggt gctgcgacag cagccgcctg acctcgtcga 1561 attcgcagtg gagtacttca cccgcctgcg cgaggcccgc gccccagcct cagtcctgcc 1621 cgccgccacc ccacgccaga gcctgggcca ccccccgcca gaacccggcc cggaccgtgt 1681 cgccgacgcc aaaggggaca gcgagtcgga ggaggacgag gacttggaag ttccagttcc 1741 tagcagattt aatagacgag tatcagtctg tgctgagacc tataaccctg atgaggaaga 1801 ggaagataca gatccaaggg tgattcatcc taaaactgat gaacagagat gcagacttca 1861 ggaagcttgc aaagatattc tccttttcaa aaatcttgat caggaacagc tttctcaagt 1921 tctcgatgcc atgtttgaaa ggatagtcaa agctgatgag catgtcattg accaaggaga 1981 tgatggagac aacttttatg tcatagaacg gggaacttat gacattttag taacaaaaga 2041 taatcaaacc cgctctgttg gtcaatatga caaccgtggc agttttggag aactagctct 2101 gatgtacaac accccgagag ctgctaccat tgttgctacc tcagaaggct ccctttgggg 2161 actggaccgg gtgactttta gaagaatcat agtgaaaaat aatgcaaaga agaggaagat 2221 gtttgaatca tttattgagt ctgtgcccct ccttaaatca ctagaggtgt cagaacgaat 2281 gaagattgtg gatgtaatag gagagaagat ctataaggat ggagaacgca taatcactca 2341 gggtgaaaag gctgatagct tttacatcat agagtctggc gaagtgagca tcttgattag 2401 aagcaggact aaatcaaaca aggatggtgg gaaccaggag gtcgagattg cccgctgcca 2461 taaggggcag tactttggag agcttgccct ggtcaccaac aaacccagag ctgcctcagc 2521 ttatgcagtt ggagatgtca aatgcttagt tatggatgta caagcattcg agaggcttct 2581 ggggccctgc atggacatca tgaagaggaa catctcacac tatgaggaac agctggtgaa 2641 gatgtttggc tccagcgtgg atctgggcaa cctcgggcag GGATCCGACT ACAAAGACCA 2701 TGACGGTGAT TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGTAGGAATT 2761 CACCGGTTAG TAAGCttatc gataatcaac ctctggatta caaaatttgt gaaagattga 2821 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 2881 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 2941 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 3001 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 3061 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 3121 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat 3181 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct 3241 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3301 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3361 ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc atccctgtga 3421 cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca ccagccttgt 3481 cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata atattatggg 3541 gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta gggcctgcgg 3601 ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg caatctccgc 3661 ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat tccaggcatg 3721 catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca ccatattggc 3781 caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc ccaaattgct 3841 gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt aggtaaccac 3901 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc 3961 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 4021 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt 4081 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 4141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 4201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 4261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 4321 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 4381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4441 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 4501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4561 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 4621 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 4681 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 4741 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 4801 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 4861 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 4921 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 4981 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 5041 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 5101 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 5161 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 5221 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 5281 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 5341 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 5401 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 5461 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 5521 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 5581 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 5641 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 5701 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 5761 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 5821 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 5881 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 5941 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6001 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6061 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6121 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 6181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 6301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 6361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 6421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 6481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 6541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 6601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.