pCMV-Il12rb1-P2A-mCherry-Puro

pCMV-Il12rb1-P2A-mCherry-Puro plasmid

PVT94369

pCMV-Il12rb1-P2A-mCherry-Puro plasmid Information

Plasmid name: pCMV-Il12rb1(mouse)-P2A-mCherry-Puro
Alias: NM_008353.2;CD212; IL-12R[b]
Gene lenght: 2214bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: mCherry-R
Copy number: High
Induction:

pCMV-Il12rb1-P2A-mCherry-Puro plasmid Description

pCMV-Il12rb1-P2A-mCherry-Puro is a plasmid suitable for protein expression in mammalian cells.

pCMV-Il12rb1-P2A-mCherry-Puro plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 Cgccaccatg gacatgatgg ggctggcagg gaccagcaaa cacatcacct tcctcctgct 961 ttgccagcta ggcgcctcag gcccaggtga tggctgctgc gttgagaaga catcgttccc 1021 agagggagcc tcaggctcac ccttaggacc caggaacttg agttgctaca gggtttccaa 1081 gacagactat gagtgctcct ggcagtatga tggccctgag gacaatgttt ctcacgtcct 1141 gtggtgctgc tttgtccctc cgaaccatac ccacaccggc caggagcgct gccgctactt 1201 ctcctcaggc ccagaccgca ctgtgcagtt ctgggaacag gacggtatcc ctgtgctgtc 1261 caaggtcaac ttctgggtgg agtctcggct tgggaaccga accatgaagt cccagaagat 1321 atcccagtac ctgtacaact ggaccaagac gacccctccc ctgggacaca tcaaggtgtc 1381 acaatcacac cggcagttgc gaatggactg gaatgtgtct gaagaggccg gtgctgaggt 1441 acagttcagg cgccgtatgc ccacaacgaa ttggaccttg ggtgactgcg gacctcaggt 1501 taactctggc tcaggtgtgc ttggtgacat tcgtgggagc atgtctgagt cctgcctctg 1561 cccttctgag aacatggccc aagagatcca gatacggagg aggaggcggc tctcctcagg 1621 agcccctgga ggtccctgga gtgattggag catgcctgtg tgtgttccac ctgaagtcct 1681 tccccaggcc aagattaagt tcttggtgga gcccctgaac caaggtggaa ggaggcgtct 1741 aaccatgcaa ggacagtcac cacagctggc agtcccggaa ggctgccgag gcaggcctgg 1801 tgcgcaggtg aagaagcact tggtgctggt gcgcatgctg tcctgcaggt gccaggctca 1861 gacctcgaag accgtgcccc tgggcaagaa gctgaacctc tccggggcca cctatgacct 1921 gaatgtgctc gccaaaactc gtttcggtcg cagcaccatc cagaagtggc accttcctgc 1981 ccaagagctc acagagacaa gagccctgaa tgtcagcgtg ggaggcaaca tgacatccat 2041 gcagtgggca gcccaggctc ccggcaccac ctactgcctt gagtggcagc catggttcca 2101 gcacaggaac cacacacact gtaccctgat tgtaccagaa gaagaggatc ctgccaagat 2161 ggtgacacac agctggagct ctaaacctac cctggagcag gaggaatgtt accgcatcac 2221 agtgttcgcc tccaagaacc ccaagaatcc catgctgtgg gccacagtcc tgtccagtta 2281 ctactttggg ggtaacgcct cgagagccgg tactccacga cacgtgtcgg tgaggaacca 2341 aaccggggac tcggtgtccg tggagtggac ggcgtcacag ctgagcacct gcccgggggt 2401 cctgacgcaa tacgtcgtgc gctgcgaggc tgaagacggc gcgtgggagt cagagtggct 2461 cgtgccaccc actaaaaccc aagtgacact tgacggactg cgcagccgag taatgtacaa 2521 ggttcaggtg cgagccgaca ctgcgaggct cccgggtgcc tggagtcatc cccagcgctt 2581 tagctttgag gtgcagattt cccgtttatc catcattttc gcgtctctgg gaagcttcgc 2641 cagcgtcctc ctcgtgggca gtctcggata cattggctta aacagggccg cctggcactt 2701 gtgcccaccc ctgcctacac cctgtggcag cactgccgtg gagttccctg gcagccaggg 2761 caagcaggct tggcagtggt gcaaccctga ggacttcccg gaggtgttgt acccgcgaga 2821 tgcgctggtg gtcgagatgc ccggagacag aggcgacggg acagagtcgc cccaggccgc 2881 ccctgagtgc gccctggaca caaggcggcc cttggagact cagaggcaga ggcaggtgca 2941 ggcactgtca gaggccaggc gcctgggcct ggctagggag gactgtcccc gtggtgacct 3001 ggcccacgtg acactcccgc tgctcctggg aggtgtgacc cagggagcct ctgtacttga 3061 cgatctttgg aggacccata agactgcgga gccgggaccg cccactttgg ggcaagaggc 3121 cGAATTCGCC ACTAACTTCT CCCTGTTGAA ACAAGCAGGG GATGTCGAAG AGAATCCCGG 3181 GCCAatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt 3241 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga 3301 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct 3361 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa 3421 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga 3481 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca 3541 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc 3601 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga 3661 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga 3721 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa 3781 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta 3841 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtagCTCGA 3901 GTCTAGAGGG CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC 3961 ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT 4021 CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT 4081 GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC 4141 TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG 4201 GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG 4261 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT 4321 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT 4381 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG 4441 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT 4501 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT 4561 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA 4621 AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA 4681 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 4741 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 4801 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 4861 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 4921 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 4981 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 5041 TTTCGCatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg 5101 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac 5161 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 5221 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 5281 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 5341 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 5401 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 5461 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 5521 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 5581 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 5641 gcctgaGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC 5701 GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG 5761 ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA 5821 ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA 5881 ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT 5941 ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT 6001 TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA 6061 AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC 6121 TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG 6181 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 6241 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 6301 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 6361 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 6421 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 6481 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 6541 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 6601 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 6661 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 6721 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 6781 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 6841 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 6901 CCGGCAAACA AACCACCGCT GGTAGCGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 6961 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 7021 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 7081 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 7141 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 7201 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 7261 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 7321 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 7381 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 7441 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 7501 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 7561 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 7621 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 7681 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 7741 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 7801 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 7861 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 7921 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 7981 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 8041 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 8101 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCGACGGA TCGGGAGATC 8161 TCCCGATCCC CTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.