pCMV-3×FLAG-CRIM1-Neo plasmid

pCMV-3×FLAG-CRIM1-Neo plasmid

PVT94364

pCMV-3×FLAG-CRIM1-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-CRIM1(human)-Neo
Alias: NM_016441.3;CRIM-1; S52
Gene lenght: 3111bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-CRIM1-Neo plasmid Description

pCMV-3×FLAG-CRIM1-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-CRIM1-Neo plasmid Sequence

1 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT 61 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG 121 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTGATCT ATACATTGAA 181 TCAATATTGG CAATTAGCCA TATTAGTCAT TGGTTATATA GCATAAATCA ATATTGGCTA 241 TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC ATTTATATTG GCTCATGTCC 301 AATATGACCG CCATGTTGAC ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG 361 GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC 421 GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 481 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC 541 CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG CCCCCTATTG ACGTCAATGA 601 CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC TTACGGGACT TTCCTACTTG 661 GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC 721 CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 781 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAATAACCC 841 CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC 901 TCGTTTAGTG AACCGTCAGA ATTAaccatg gactacaaag accatgacgg tgattataaa 961 gatcatgaca tcgattacaa ggatgacgat gacAAGCTTa tgtacttggt ggcgggggac 1021 agggggttgg ccggctgcgg gcacctcctg gtctcgctgc tggggctgct gctgctgctg 1081 gcgcgctccg gcacccgggc gctggtctgc ctgccctgtg acgagtccaa gtgcgaggag 1141 cccaggaact gcccggggag catcgtgcag ggcgtctgcg gctgctgcta cacgtgcgcc 1201 agccagagga acgagagctg cggcggcacc ttcgggattt acggaacctg cgaccggggg 1261 ctgcgttgtg tcatccgccc cccgctcaat ggcgactccc tcaccgagta cgaagcgggc 1321 gtttgcgaag atgagaactg gactgatgac caactgcttg gttttaaacc atgcaatgaa 1381 aaccttattg ctggctgcaa tataatcaat gggaaatgtg aatgtaacac cattcgaacc 1441 tgcagcaatc cctttgagtt tccaagtcag gatatgtgcc tttcagcttt aaagagaatt 1501 gaagaagaga agccagattg ctccaaggcc cgctgtgaag tccagttctc tccacgttgt 1561 cctgaagatt ctgttctgat cgagggttat gctcctcctg gggagtgctg tcccttaccc 1621 agccgctgcg tgtgcaaccc cgcaggctgt ctgcgcaaag tctgccagcc gggaaacctg 1681 aacatactag tgtcaaaagc ctcagggaag ccgggagagt gctgtgacct ctatgagtgc 1741 aaaccagttt tcggcgtgga ctgcaggact gtggaatgcc ctcctgttca gcagaccgcg 1801 tgtcccccgg acagctatga aactcaagtc agactaactg cagatggttg ctgtactttg 1861 ccaacaagat gcgagtgtct ctctggctta tgtggtttcc ccgtgtgtga ggtgggatcc 1921 actccccgca tagtctctcg tggcgatggg acacctggaa agtgctgtga tgtctttgaa 1981 tgtgttaatg atacaaagcc agcctgcgta tttaacaatg tggaatatta tgatggagac 2041 atgtttcgaa tggacaactg tcggttctgt cgatgccaag ggggcgttgc catctgcttc 2101 accgcccagt gtggtgagat aaactgcgag aggtactacg tgcccgaagg agagtgctgc 2161 ccagtgtgtg aagatccagt gtatcctttt aataatcccg ctggctgcta tgccaatggc 2221 ctgatccttg cccacggaga ccggtggcgg gaagacgact gcacattctg ccagtgcgtc 2281 aacggtgaac gccactgcgt tgcgaccgtc tgcggacaga cctgcacaaa ccctgtgaaa 2341 gtgcctgggg agtgttgccc tgtgtgcgaa gaaccaacca tcatcacagt tgatccacct 2401 gcatgtgggg agttatcaaa ctgcactctg acagggaagg actgcattaa tggtttcaaa 2461 cgcgatcaca atggttgtcg gacctgtcag tgcataaaca ccgaggaact atgttcagaa 2521 cgtaaacaag gctgcacctt gaactgtccc ttcggtttcc ttactgatgc ccaaaactgt 2581 gagatctgtg agtgccgccc aaggcccaag aagtgcagac ccataatctg tgacaagtat 2641 tgtccacttg gattgctgaa gaataagcac ggctgtgaca tctgtcgctg taagaaatgt 2701 ccagagctct catgcagtaa gatctgcccc ttgggtttcc agcaggacag tcacggctgt 2761 cttatctgca agtgcagaga ggcctctgct tcagctgggc cacccatcct gtcgggcact 2821 tgtctcaccg tggatggtca tcatcataaa aatgaggaga gctggcacga tgggtgccgg 2881 gaatgctact gtctcaatgg acgggaaatg tgtgccctga tcacctgccc ggtgcctgcc 2941 tgtggcaacc ccaccattca ccctggacag tgctgcccat catgtgcaga tgactttgtg 3001 gtgcagaagc cagagctcag tactccctcc atttgccacg cccctggagg agaatacttt 3061 gtggaaggag aaacgtggaa cattgactcc tgtactcagt gcacctgcca cagcggacgg 3121 gtgctgtgtg agacagaggt gtgcccaccg ctgctctgcc agaacccctc acgcacccag 3181 gattcctgct gcccacagtg tacagatcaa ccttttcggc cttccttgtc ccgcaataac 3241 agcgtaccta attactgcaa aaatgatgaa ggggatatat tcctggcagc tgagtcctgg 3301 aagcctgacg tttgtaccag ctgcatctgc attgatagcg taattagctg tttctctgag 3361 tcctgccctt ctgtatcctg tgaaagacct gtcttgagaa aaggccagtg ttgtccctac 3421 tgcatagaag acacaattcc aaagaaggtg gtgtgccact tcagtgggaa ggcctatgcc 3481 gacgaggagc ggtgggacct tgacagctgc acccactgct actgcctgca gggccagacc 3541 ctctgctcga ccgtcagctg cccccctctg ccctgtgttg agcccatcaa cgtggaagga 3601 agttgctgcc caatgtgtcc agaaatgtat gtcccagaac caaccaatat acccattgag 3661 aagacaaacc atcgaggaga ggttgacctg gaggttcccc tgtggcccac gcctagtgaa 3721 aatgatatcg tccatctccc tagagatatg ggtcacctcc aggtagatta cagagataac 3781 aggctgcacc caagtgaaga ttcttcactg gactccattg cctcagttgt ggttcccata 3841 attatatgcc tctctattat aatagcattc ctattcatca atcagaagaa acagtggata 3901 ccactgcttt gctggtatcg aacaccaact aagccttctt ccttaaataa tcagctagta 3961 tctgtggact gcaagaaagg aaccagagtc caggtggaca gttcccagag aatgctaaga 4021 attgcagaac cagatgcaag attcagtggc ttctacagca tgcaaaaaca gaaccatcta 4081 caggcagaca atttctacca aacagtgtga GAATTCATCG ATAGATCTGA TATCGGTACC 4141 AGTCGACTCT AGAGGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG 4201 CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA 4261 TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA 4321 AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA 4381 GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC 4441 TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT 4501 TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT 4561 CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG 4621 CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC 4681 AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG 4741 TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCTT 4801 GGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 4861 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC 4921 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC 4981 TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT 5041 GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 5101 AGTAGTGAGG AGGCTTTTTT GGAGGAATTG ATCAGCTTGG GATCTGATCA AGAGACAGGA 5161 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 5221 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 5281 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 5341 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 5401 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 5461 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 5521 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 5581 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 5641 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 5701 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 5761 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG 5821 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 5881 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 5941 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 6001 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 6061 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 6121 TGCTGGAGTT CTTCGCCCAC CCCGGGCTCG ATCCCCTCGC GAGTTGGTTC AGCTGCTGCC 6181 TGAGGCTGGA CGACCTCGCG GAGTTCTACC GGCAGTGCAA ATCCGTCGGC ATCCAGGAAA 6241 CCAGCAGCGG CTATCCGCGC ATCCATGCCC CCGAACTGCA GGAGTGGGGA GGCACGATGG 6301 CCGCTTTGGT CGACCCGGAC GGGACGCTCC TGCGCCTGAT ACAGAACGAA TTGCTTGCAG 6361 GCATCTCATG AGTGTGTCTT CCCGTTTTCC GCCTGAGGTC ACTGCGTGGA TGGAGCGCTG 6421 GCGCCTGCTG CGCGACGGCG AGCTGCTCAC CACCCACTCG CCAAGCTGGA ACCGTAAAAA 6481 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCGATCA TAATCAGCCA TACCACATTT 6541 GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA 6601 ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC 6661 AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG 6721 TCCAAACTCA TCAATGTATC TTATCATGTC TGGATCAATT CCCTATAGTG AGTCGTATTA 6781 AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 6841 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 6901 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 6961 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 7021 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 7081 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 7141 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 7201 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 7261 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 7321 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 7381 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 7441 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 7501 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 7561 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 7621 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 7681 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 7741 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 7801 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 7861 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 7921 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 7981 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 8041 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 8101 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 8161 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 8221 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 8281 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 8341 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 8401 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 8461 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 8521 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 8581 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 8641 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 8701 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 8761 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 8821 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 8881 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 8941 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 9001 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 9061 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 9121 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 9181 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 9241 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 9301 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 9361 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.