pLV3-CMV-MIR1260A-EF1a-CopGFP-

pLV3-CMV-MIR1260A-EF1a-CopGFP-Puro plasmid

PVT94352

pLV3-CMV-MIR1260A-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-MIR1260A(human)-EF1a-CopGFP-Puro
Alias:
Gene lenght: 486bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MIR1260A-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-MIR1260A-EF1a-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MIR1260A-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc CCACCAAGAA GGCTAAAGCT GCTCTTTCTC 2341 TTTATTTGTG AAAAGCAAGC CATCTTGGGG CAGGAAAGCA GTGAAGGGAC TGGGCAGGCA 2401 GGACAGAAGG CGCTGGAAGC TCAGCCATCT CACCCCCGCC TTGGCCTGCA CTCACCTGAA 2461 GAAGCAGGAC AGACAGAAGA GCCCCATCCT CTCCCACCTC CATACCTTTC CAGCTCATCC 2521 CACCTCTGCC ACCAAAACAC TCATCGCGGG GTCAGAGGGA GTGCCAAAAA AGGTAAAAAG 2581 AAACGCAAGA AAGAGGCTAC TGGGGATGAG GGGACACCCA GAAGCCCCTT CCTGGAGGAA 2641 AAGCCACTCC TTCTGCAGCT GGACTCTAGG ATACTGAGAC CCAGCCCTGC CCCATCACCT 2701 CTCCAGTGTG GCCAAGGGGA CAAGGACCAA GATGCAGGGA AGCTTGGGGA GCCTGGGCTA 2761 CAACAGATAC AGGCCAACAG ACAGACACAG ATGGATggat ccCTGCGATA TCCAGCgcgg 2821 ccgcGaagga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 2881 gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc 2941 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 3001 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 3061 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc 3121 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc 3181 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg 3241 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt 3301 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga 3361 ccggcgccta cgctagacgc caccatggag agcgacgaga gcggcctgcc cgccatggag 3421 atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt gggcggcgga 3481 gagggcaccc ccaagcaggg ccgcatgacc aacaagatga agagcaccaa aggcgccctg 3541 accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca cttcggcacc 3601 taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg ctacaccaac 3661 acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag ctaccgctac 3721 gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca ccggcttccc cgaggacagc 3781 gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct gcaccccatg 3841 ggcgataacg tgctggtggg cagcttcgcc cgcaccttca gcctgcgcga cggcggctac 3901 tacagcttcg tggtggacag ccacatgcac ttcaagagcg ccatccaccc cagcatcctg 3961 cagaacgggg gccccatgtt cgccttccgc cgcgtggagg agctgcacag caacaccgag 4021 ctgggcatcg tggagtacca gcacgccttc aagaccccca tcgccttcgc cagatcccgc 4081 gctcagtcgt ccaattctgc cgtggacggc accgccggac ccggctccac cggatctcgc 4141 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctatgacc 4201 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 4261 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 4321 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 4381 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 4441 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 4501 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 4561 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4621 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 4681 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 4741 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaaatcaa 4801 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 4861 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 4921 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 4981 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 5041 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 5101 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 5161 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 5221 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 5281 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tctccgcctt 5341 cgccctcaga cgagtcggat ctccctttgg ccgcctcccc gcctggtacc tttaagacca 5401 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 5461 gggctaattc actcccaacg aaaataagat ctgctttttg cttgtactgg gtctctctgg 5521 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 5581 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 5641 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 5701 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 5761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 5821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 5881 tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc cattctccgc 5941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 6001 tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga gacggcccaa 6061 attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 6121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 6181 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 6241 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 6301 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 6361 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 6421 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 6481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 6541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 6601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 6661 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 6721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6901 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 6961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7141 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7201 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7261 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 7321 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 7381 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 7441 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 7501 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 7561 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 7621 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7681 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7741 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7801 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7861 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7921 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7981 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8041 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8101 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8221 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 8281 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 8341 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 8401 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 8461 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 8521 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 8581 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 8641 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.