pLV2-CMV-Tet3G-U6-DDX17-sgRNA3

pLV2-CMV-Tet3G-U6-DDX17-sgRNA3-Neo plasmid

PVT94184

pLV2-CMV-Tet3G-U6-DDX17-sgRNA3-Neo plasmid Information

Plasmid name: pLV2-CMV-Tet3G-U6-DDX17(human)-sgRNA3-Neo
Alias: ATGGTGGGCATTGCTCAGAC
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing,Tetracycline induction
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-CMV-Tet3G-U6-DDX17-sgRNA3-Neo plasmid Description

pLV2-CMV-Tet3G-U6-DDX17-sgRNA3-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-Tet3G-U6-DDX17-sgRNA3-Neo plasmid Sequence

1 ACTACTAAGT TTGTAGTACA TATTTAACAA ATACAATTTC TTTAAAAACT ACTAAGTTTG 61 TAGTACATAT TTAACAAATA CAATTTCTTT AAAATGAAAA TAATTCAGAG GAATCACAGG 121 TTTAGAGTAA ATGAAACCAC TAGGTAATTG GCAGTGGTAA TAGGGTATGG GGTGGGAAGT 181 TTGGGATGAT TTTGGTTAGC TTGAGTTATC CAGTTGATCC AGACATGATA AGATACATTG 241 ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT 301 GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT GATCTCCCGA 361 TCCGTCGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT 421 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA 481 ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT 541 TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA 601 TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA 661 GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT 721 GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT 781 ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA 841 TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC 901 CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT 961 GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA 1021 CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC 1081 TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA 1141 AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC 1201 TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC 1261 CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG 1321 ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA 1381 CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA 1441 GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC 1501 GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT 1561 CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA 1621 GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT 1681 TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA 1741 CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC 1801 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG 1861 TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG 1921 TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG 1981 CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT 2041 TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC 2101 AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT 2161 TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG 2221 TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA 2281 GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG 2341 GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG 2401 CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT 2461 TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA 2521 TGACCATGAT TACGCCAAGC TCTAGCTAGA GGTCGACGGT AAAACGCTAG CAATGTCAAG 2581 GCCTCTCACT CTCTGATATT CATTTCTTTG CAAGTTATAA ATACTGAATA ATAAGATGAC 2641 ATGAACTACT ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT 2701 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT 2761 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA 2821 ACCAGAGAGA CCCAGTACAG GCAAAAAGCA GCTGCTTATA TGCAGGATCT GAGGGCTCGC 2881 CACTCCCCAG TCCCGCCCAG GCCACGCCTC CCTGGAAAGT CCCCAGCGGA AAGTCCCTTG 2941 TAGCAAGCTC GATATCAGCA GTTCTTGAAG TACTCCGGAT GCAGCTCTCG GGCCACGTGA 3001 TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAACACTT CTCTCTCCGG GTCATCCATC 3061 CCATGCAGGC TCACAGGGTG TAACAAGCTG GTGTTCTCTC CTTTATTGGC CTCTTCTACC 3121 TTATCTGGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGACC 3181 CCTGGCCCTG GTGTGTAGTT CTGCTAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC 3241 AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCTCTTTTCT 3301 TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA 3361 GGTGTGACTG GAAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG CACAATCAGC 3421 ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT CTCGACTGCA 3481 GAATTAATTC CAGGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG 3541 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA 3601 TTGAGGGCCG AAGGGACGTA GCAGAAGGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG 3661 GCGAGCAGCC ATGGAAAGGA CGTCAGCTTC CCCGACAACA CCACGGAATT GTCAGTGCCC 3721 AACAGCCGAG CCCCTGTCCA GCAGCGGGCA AGGCAGGCGG CGATGAGTTC CGCCGTGGCA 3781 ATAGGGAGGG GGAAAGCGAA AGTCCCGGAA AGGAGCTGAC AGGTGGTGGC AATGCCCCAA 3841 CCAGTGGGGG TTGCGTCAGC AAACACAGTG CACACCACGC CACGTTGCCT GACAACGGGC 3901 CACAACTCCT CATAAAGAGA CAGCAACCAG GATTTATACA AGGAGGAGAA AATGAAAGCC 3961 ATACGGGAAG CAATAGCATG ATACAAAGGC ATTAAAGCAG CGTATCCACA TAGCGTAAAA 4021 GGAGCAACAT AGTTAAGAAT ACCAGTCAAT CTTTCACAAA TTTTGTAATC CAGAGGTTGA 4081 TTGTTCCAGA CGCGTTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA 4141 ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC 4201 TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA CACCCAGCCG 4261 GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC 4321 ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA GCCTGGCGAA 4381 CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC 4441 GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA 4501 GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC 4561 GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA 4621 GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC 4681 CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT 4741 CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA 4801 GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA 4861 ACCTGCGTGC AATCCATCTT GTTCAGCCAT GGTTGTGGCA AGCTTATCAT CGTGTTTTTC 4921 AAAGGAAAAC CACGTCCCCG TGGTTCGGGG GGCCTAGACG TTTTTTAACC TCGACTAAAC 4981 ACATGTAAAG CATGTGCACC GAGGCCCCAG ATCAGATCCC ATACAATGGG GTACCTTCTG 5041 GGCATCCTTC AGCCCCTTGT TGAATACGCT TGAGGAGAGC CATTTGACTC TTTCCACAAC 5101 TATCCAACTC ACAACGTGGC ACTGGGGTTG TGCCGCCTTT GCAGGTGTAT CTTATACACG 5161 TGGCTTTTGG CCGCAGAGGC ACCTGTCGCC AGGTGGGGGG TTCCGCTGCC TGCAAAGGGT 5221 CGCTACAGAC GTTGTTTGTC TTCAAGAAGC TTCCAGAGGA ACTGCTTCCT TCACGACATT 5281 CAACAGACCT TGCATTCCTT TGGCGAGAGG GGAAAGACCC CTAGGAATGC TCGTCAAGAA 5341 GACAGGGCCA GGTTTCCGGG CCCTCACATT GCCAAAAGAC GGCAATATGG TGGAAAATAA 5401 CATATAGACA AACGCACACC GGCCTTATTC CAAGCGGCTT CGGCCAGTAA CGTTAGGGGG 5461 GGGGGAGGGA GAGGGGCGGG ATCCTTACCC GGGGAGCATG TCAAGGTCAA AATCGTCAAG 5521 AGCGTCAGCA GGCAGCATAT CAAGGTCAAA GTCGTCAAGG GCATCGGCTG GGAGCATGTC 5581 TAAGTCAAAA TCGTCAAGGG CGTCGGTCGG CCCGCCGCTT TCGCACTTTA GCTGTTTCTC 5641 CAGGCCACAT ATGATTAGTT CCAGGCCGAA AAGGAAGGCA GGTTCGGCTC CCTGCCGGTC 5701 GAACAGCTCA ATTGCTTGTT TCAGAAGTGG GGGCATAGAA TCGGTGGTAG GTGTCTCTCT 5761 TTCCTCTTTT GCTACTTGAT GCTCCTGTTC CTCCAATACG CAGCCCAGTG TAAAGTGGCC 5821 CACGGCGGAC AGAGCGTACA GTGCGTTCTC CAGGGAGAAG CCTTGCTGAC ACAGGAACGC 5881 GAGCTGATTT TCCAGGGTTT CGTACTGTTT CTCTGTTGGG CGGGTGCCGA GATGCACTTT 5941 AGCCCCGTCG CGATGTGAGA GGAGAGCACA GCGGTATGAC TTGGCGTTGT TCCGCAGAAA 6001 GTCTTGCCAT GACTCGCCTT CCAGGGGGCA GGAGTGGGTA TGATGCCTGT CCAGCATCTC 6061 GATTGGCAGG GCATCGAGCA GGGCCCGCTT GTTCTTCACG TGCCAGTACA GGGTAGGCTG 6121 CTCAACTCCC AGCTTTTGAG CGAGTTTCCT TGTCGTCAGG CCTTCGATAC CGACTCCATT 6181 GAGTAATTCC AGAGCAGAGT TTATGACTTT GCTCTTGTCC AGTCTAGACA TGGTGGCCTC 6241 GAGGAATTCA CCGGAAATAG ATCCTCTAGT AGAGTCGGTG TCTTCTATGG AGGTCAAAAC 6301 AGCGTGGATG GTCTCCAGGC GATCTGACGG TTCACTAAAC GAGCTCTGCT TATATAGACC 6361 TCCCACCGTA CACGCCTACC GCCCATTTGC GTCAATGGGG CGGAGTTGTT ACGACATTTT 6421 GGAAAGTCCC GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA 6481 CTTGGAAATC CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC 6541 ATCACCATGG TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT 6601 AAGGTCATGT ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG 6661 GGCGTACTTG GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC 6721 TCCACCCATT GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT 6781 ATTGACGTCA ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT 6841 TATGTAACGC GGAACTCCCA AGCTTGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA 6901 TATACGATAC AAGGCTGTTA GAGAGATAAT TGGAATTAAT TTGACTGTAA ACACAAAGAT 6961 ATTAGTACAA AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA 7021 ATTATGTTTT AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT 7081 GGCTTTATAT ATCTTGTGGA AAGGACGAAA CACCGATGGT GGGCATTGCT CAGACGTTTT 7141 AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC 7201 CGAGTCGGTG CTTTTTTTTT ATCGATAAAC TGGATCTCTG CTGTCCCTGT AATAAACCCG 7261 AAAATTTTGA ATTTTTGTAA TTTGTTTTTG TAGTTCTTTA GTTTGTATGT CTGTTGCTAT 7321 TATGTCTACT ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAGGC 7381 GATACCGTCG AGATCCGTTC ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT 7441 CTTCTTCTAT TCCTTCGGGC CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG 7501 ATAATGGTGA ATATCCCTGC CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC 7561 TTAAACCTAC CAAGCCTCCT ACTATCATTA TGAATAATTT TATATACCAC AGCCAATTTG 7621 TTATGTTAAA CCAATTCCAC AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT 7681 TCTTTTCTTG CTGGTTTTGC GATTCTTCAA TTAAGGAGTG TATTAAGCTT GTGTAATTGT 7741 TAATTTCTCT GTCCCACTCC ATCCAGGTCG TGTGATTCCA AATCTGTTCC AGAGATTTAT 7801 TACTCCAACT AGCATTCCAA GGCACAGCAG TGGTGCAAAT GAGTTTTCCA GAGCAACCCC 7861 AAATCCCCAG GAGCTGTTGA TCCTTTAGGT ATCTTTCCAC AGCCAGGATT CTTGCCTGGA 7921 GCTGCTTGAT GCCCCAGACT GTGAGTTGCA ACAGATGCTG TTGCGCCTCA ATAGCCCTCA 7981 GCAAATTGTT CTGCTGCTGC ACTATACCAG ACAATAATTG TCTGGCCTGT ACCGTCAGCG 8041 TCATTGACGC TGCGCCCATA GTGCTTCCTG CTGCTCCCAA GAACCCAAGG AACAAAGCTC 8101 CTATTCCCAC TGCTCTTTTT TCTCTCTGCA CCACTCTTCT CTTTGCCTTG GTGGGTGCTA 8161 CTCCTAATGG TTCAATTTTT ACTACTTTAT ATTTATATAA TTCACTTCTC CAATTGTCCC 8221 TCATATCTCC TCCTCCAGGT CTGAAGATCA GCGGCCGGCC GCTTGCTGTG CGGTGGTCTT 8281 ACTTTTGTTT TGCTCTTCCT CTATCTTGTC TAAAGCTTCC TTGGTGTCTT TTATCTCTAT 8341 CCTTTGATGC ACACAATAGA GGGTTGCTAC TGTATTATAT AATGATCTAA GTTCTTCTGA 8401 TCCTGTCTGA AGGGATGGTT GTAGCTGTCC CAGTATTTGT CTACAGCCTT CTGATGTTTC 8461 TAACAGGCCA GGATTAACTG CGAATCGTTC TAGCTCCCTG CTTGCCCATA CTATATGTTT 8521 TAATTTATAT TTTTTCTTTC CCCCTGGCCT TAACCGAATT TTTTCCCATC GCGATCTAAT 8581 TCTCCCCCGC TTAATACTGA CGCTCTCGCA CCCATCTCTC TCCTTCTAGC CTCCGCTAGT 8641 CAAAATTTTT GGCGTACTCA CCAGTCGCCG CCCCTCGCCT CTTGCCGTGC GCGCTTCAGC 8701 AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT TTCGCTTTCA AGTCCCTGTT 8761 CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA 8821 CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA 8881 GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC 8941 CAGAGAGACC CAGTACAGGC AAAAAGCAGC TGCTTATATG CAGGATCTGA GGGCTCGCCA 9001 CTCCCCAGTC CCGCCCAGGC CACGCCTCCC TGGAAAGTCC CCAGCGGAAA GTCCCTTGTA 9061 GCAAGCTCGA TATCAGCAGT TCTTGAAGTA CTCCGGATGC AGCTCTCGGG CCACGTGATG 9121 AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAACACTTCT CTCTCCGGGT CATCCATCCC 9181 ATGCAGGCTC ACAGGGTGTA ACAAGCTGGT GTTCTCTCCT TTATTGGCCT CTTCTACCTT 9241 ATCTGGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT ATCTGACCCC 9301 TGGCCCTGGT GTGTAGTTCT GCTAATCAGG GAAGTAGCCT TGTGTGTGGT AGATCCACAG 9361 ATCAAGGATA TCTTGTCTTC TTTGGGAGTG AATTAGCCCT TCCA

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.