pTac-GST-SIRT1 plasmid

pTac-GST-SIRT1 plasmid

PVT94027

pTac-GST-SIRT1 plasmid Information

Plasmid name: pTac-GST-SIRT1(human)
Alias: NM_012238.5;SIR2; SIR2alpha; SIR2L1
Gene lenght: 2244bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-SIRT1 plasmid Description

pTac-GST-SIRT1 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-SIRT1 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggc ggacgaggcg gccctcgccc 961 ttcagcccgg cggctccccc tcggcggcgg gggccgacag ggaggccgcg tcgtcccccg 1021 ccggggagcc gctccgcaag aggccgcgga gagatggtcc cggcctcgag cggagcccgg 1081 gcgagcccgg tggggcggcc ccagagcgtg aggtgccggc ggcggccagg ggctgcccgg 1141 gtgcggcggc ggcggcgctg tggcgggagg cggaggcaga ggcggcggcg gcaggcgggg 1201 agcaagaggc ccaggcgact gcggcggctg gggaaggaga caatgggccg ggcctgcagg 1261 gcccatctcg ggagccaccg ctggccgaca acttgtacga cgaagacgac gacgacgagg 1321 gcgaggagga ggaagaggcg gcggcggcgg cgattgggta ccgagataac cttctgttcg 1381 gtgatgaaat tatcactaat ggttttcatt cctgtgaaag tgatgaggag gatagagcct 1441 cacatgcaag ctctagtgac tggactccaa ggccacggat aggtccatat acttttgttc 1501 agcaacatct tatgattggc acagatcctc gaacaattct taaagattta ttgccggaaa 1561 caatacctcc acctgagttg gatgatatga cactgtggca gattgttatt aatatccttt 1621 cagaaccacc aaaaaggaaa aaaagaaaag atattaatac aattgaagat gctgtgaaat 1681 tactgcaaga gtgcaaaaaa attatagttc taactggagc tggggtgtct gtttcatgtg 1741 gaatacctga cttcaggtca agggatggta tttatgctcg ccttgctgta gacttcccag 1801 atcttccaga tcctcaagcg atgtttgata ttgaatattt cagaaaagat ccaagaccat 1861 tcttcaagtt tgcaaaggaa atatatcctg gacaattcca gccatctctc tgtcacaaat 1921 tcatagcctt gtcagataag gaaggaaaac tacttcgcaa ctatacccag aacatagaca 1981 cgctggaaca ggttgcggga atccaaagga taattcagtg tcatggttcc tttgcaacag 2041 catcttgcct gatttgtaaa tacaaagttg actgtgaagc tgtacgagga gatattttta 2101 atcaggtagt tcctcgatgt cctaggtgcc cagctgatga accgcttgct atcatgaaac 2161 cagagattgt gttttttggt gaaaatttac cagaacagtt tcatagagcc atgaagtatg 2221 acaaagatga agttgacctc ctcattgtta ttgggtcttc cctcaaagta agaccagtag 2281 cactaattcc aagttccata ccccatgaag tgcctcagat attaattaat agagaacctt 2341 tgcctcatct gcattttgat gtagagcttc ttggagactg tgatgtcata attaatgaat 2401 tgtgtcatag gttaggtggt gaatatgcca aactttgctg taaccctgta aagctttcag 2461 aaattactga aaaacctcca cgaacacaaa aagaattggc ttatttgtca gagttgccac 2521 ccacacctct tcatgtttca gaagactcaa gttcaccaga aagaacttca ccaccagatt 2581 cttcagtgat tgtcacactt ttagaccaag cagctaagag taatgatgat ttagatgtgt 2641 ctgaatcaaa aggttgtatg gaagaaaaac cacaggaagt acaaacttct aggaatgttg 2701 aaagtattgc tgaacagatg gaaaatccgg atttgaagaa tgttggttct agtactgggg 2761 agaaaaatga aagaacttca gtggctggaa cagtgagaaa atgctggcct aatagagtgg 2821 caaaggagca gattagtagg cggcttgatg gtaatcagta tctgtttttg ccaccaaatc 2881 gttacatttt ccatggcgct gaggtatatt cagactctga agatgacgtc ttatcctcta 2941 gttcttgtgg cagtaacagt gatagtggga catgccagag tccaagttta gaagaaccca 3001 tggaggatga aagtgaaatt gaagaattct acaatggctt agaagatgag cctgatgttc 3061 cagagagagc tggaggagct ggatttggga ctgatggaga tgatcaagag gcaattaatg 3121 aagctatatc tgtgaaacag gaagtaacag acatgaacta tccatcaaac aaatcatagc 3181 tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg 3241 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 3301 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 3361 tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga 3421 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 3481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 3541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 3601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 3661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 3721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 3781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 3841 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 3901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 3961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 4021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 4081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 4141 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 4201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 4261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 4321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 4381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 4441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 4501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 4561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 4621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 4681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 4741 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 4801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 4861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 4921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 4981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 5041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 5101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 5161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 5221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 5281 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 5341 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 5401 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcataa 5461 attccgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag 5521 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 5581 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 5641 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 5701 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 5761 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 5821 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 5881 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 5941 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 6001 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 6061 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 6121 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 6181 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 6241 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 6301 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 6361 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt 6421 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 6481 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 6541 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 6601 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 6661 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 6721 ggataacaat ttcacacagg aaacagctat gaccatgatt acggattcac tggccgtcgt 6781 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca 6841 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 6901 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc 6961 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg 7021 gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa 7081 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga 7141 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgttg gaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.