pLV3-CMV-XKRX-3×FLAG-EF1a-mChe

pLV3-CMV-XKRX-3×FLAG-EF1a-mCherry-Neo plasmid

PVT94021

pLV3-CMV-XKRX-3×FLAG-EF1a-mCherry-Neo plasmid Information

Plasmid name: pLV3-CMV-XKRX(human)-3×FLAG-EF1a-mCherry-Neo
Alias: NM_212559.3;XKR2; XPLAC; XRG2
Gene lenght: 1347bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-XKRX-3×FLAG-EF1a-mCherry-Neo plasmid Description

pLV3-CMV-XKRX-3×FLAG-EF1a-mCherry-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-XKRX-3×FLAG-EF1a-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggacag agtttatgaa attcctgagg agccaaatgt 2341 ggatccggtt tcatctctgg aggaagatgt catccgtgga gccaaccccc gatttacttt 2401 tccatttagc atccttttct ccaccttttt gtactgtggg gaggctgcat ctgctttgta 2461 catggttaga atctatcgaa agaatagtga aacttactgg atgacataca ccttttcttt 2521 ctttatgttt tcatccatta tggtccagtt gaccctcatt tttgtccaca gagatctagc 2581 caaagataaa ccgctatcat tatttatgca tctaatcctc ttgggacctg ttatcagatg 2641 tttggaggcc atgattaagt acctcacact gtggaagaaa gaggagcagg aggagcccta 2701 tgtcagcctc acccgaaaga agatgctaat agatggcgag gaggtgctga tagaatggga 2761 ggtgggccac tccatccgga ccctggctat gcaccgcaat gcctacaaac gtatgtcaca 2821 gatccaagcc ttcctgggct cagtgcccca gctgacctat cagctctatg tgagcctgat 2881 ctctgcagag gttcccctgg gtagagttgt gctaatggta ttttccctgg tatctgtcac 2941 ctatggggcc accctttgca atatgttggc tatccagatc aagtacgatg actacaagat 3001 tcgccttggg ccactagaag tcctctgcat caccatctgg cggacattgg agatcacttc 3061 ccgcctcctg attctggtgc tcttctcagc cactttgaaa ttgaaggctg tgcccttcct 3121 agtgctcaac ttcctgatca tcctctttga gccctggatt aagttctgga gaagtggtgc 3181 ccagatgccc aataacattg agaaaaactt cagccgggtc ggcactctgg tggtcctgat 3241 ttcagtcacc atcctctatg ctggcatcaa cttctcttgc tggtcagctt tgcagttgag 3301 gttggcagac agagatctcg tcgacaaagg gcagaactgg ggacatatgg gcctgcacta 3361 tagtgtgagg ttggtagaga atgtgatcat ggtcttggtt tttaagttct ttggagtgaa 3421 agtgttactg aattactgtc attccttgat tgccttgcag ctcattattg cttatctgat 3481 ttccattggc ttcatgctcc ttttcttcca gtacttgcat ccattgcgct cactcttcac 3541 ccataatgta gtagactacc tccattgtgt ctgctgtcac cagcaccctc ggaccagggt 3601 tgagaactca gagccaccct ttgagactga agcaaggcaa agtgttgtcg gatccGACTA 3661 CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA 3721 GTAgcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 3781 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 3841 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 3901 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 3961 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 4021 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 4081 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 4141 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 4201 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 4261 gctgtgaccg gcgcctacgc tagacgccac catggtgagc aagggcgagg aggataacat 4321 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 4381 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 4441 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 4501 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 4561 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 4621 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg 4681 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 4741 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 4801 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 4861 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 4921 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 4981 catggacgag ctgtacaagg gatctcgcga gggcagagga agtcttctaa catgcggtga 5041 cgtggaggag aatcccggcc ctGCCACCat ggctgaacaa gatggattgc acgcaggttc 5101 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 5161 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 5221 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 5281 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 5341 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 5401 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 5461 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 5521 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 5581 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 5641 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 5701 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 5761 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 5821 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaGTCGACA ATCAACCTCT 5881 GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT 5941 ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT 6001 TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT 6061 CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT 6121 TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC 6181 GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA 6241 CAATTCCGTG GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC 6301 CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA 6361 CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC 6421 TCAGACGAGT CGGATCTCCC TTTGGGCCGC CTCCCCGCgg tacctttaag accaatgact 6481 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 6541 attcactccc aacgaaGata agatctgctt tttgcttgta ctgggtctct ctggttagac 6601 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 6661 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 6721 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 6781 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 6841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 6901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 6961 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 7021 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 7081 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt 7141 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 7201 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 7261 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 7321 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 7381 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 7441 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 7501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 7561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 7621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 7681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 7741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 7801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 7861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 7921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 7981 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 8041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 8101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 8161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 8221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 8281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 8341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 8401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 8461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 8521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 8581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 8641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 8701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 8761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 8821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 8881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 8941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 9001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 9061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 9121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 9181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 9241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 9301 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 9361 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 9421 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 9481 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 9541 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 9601 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 9661 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 9721 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.