pCMV-3×FLAG-PYGB-Neo plasmid

pCMV-3×FLAG-PYGB-Neo plasmid

PVT94013

pCMV-3×FLAG-PYGB-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-PYGB(human)-Neo
Alias: NM_002862.4;GPBB
Gene lenght: 2532bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: BGH
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-PYGB-Neo plasmid Description

pCMV-3×FLAG-PYGB-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-PYGB-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atggcgaagc cgctgacgga cagcgagaag 1021 cggaagcaga tcagcgtgcg cggcctggcg gggctaggcg acgtggccga ggtgcggaag 1081 agcttcaacc ggcacttgca cttcacgctg gtcaaggacc gcaatgtggc cacgccccgc 1141 gactacttct tcgcgctggc gcacacggtg cgcgaccacc tcgtgggccg ctggatccgc 1201 acgcagcagc actactacga gcgcgacccc aagcgcattt attatctttc cctggaattc 1261 tacatgggtc gcacgctgca gaacacgatg gtgaacctgg gccttcagaa tgcctgcgat 1321 gaagccatct atcagttggg gttagacttg gaggaactcg aggagataga agaagatgct 1381 ggccttggga atggaggcct ggggaggctg gcagcgtgtt tccttgactc aatggctacc 1441 ttgggcctgg cagcatacgg ctatggaatc cgctatgaat ttgggatttt taaccagaag 1501 attgtcaatg gctggcaggt agaggaggcc gatgactggc tgcgctacgg caacccctgg 1561 gagaaagcgc ggcctgagta tatgcttccc gtgcacttct acggacgcgt ggagcacacc 1621 cccgacggcg tgaagtggct ggacacacag gtggtgctgg ccatgcccta cgacacccca 1681 gtgcccggct acaagaacaa caccgtcaac accatgcggc tgtggtccgc caaggctccc 1741 aacgacttca agctgcagga cttcaacgtg ggagactaca tcgaggcggt cctggaccgg 1801 aacttggctg agaacatctc cagggtcctg tatccaaatg ataacttctt tgaggggaag 1861 gagctgcggc tgaagcagga gtacttcgtg gtggccgcca cgctccagga catcatccgc 1921 cgcttcaagt cgtccaagtt cggctgccgg gaccctgtga gaacctgttt cgagacgttc 1981 ccagacaagg tggccatcca gctgaacgac acccaccccg ccctctccat ccctgagctc 2041 atgcggatcc tggtggacgt ggagaaggtg gactgggaca aggcctggga aatcacgaag 2101 aagacctgtg catacaccaa ccacactgtg ctgcctgagg ccttggagcg ctggcccgtg 2161 tccatgtttg agaagctgct gccgcggcac ctggagataa tctatgccat caaccagcgg 2221 cacctggacc acgtggccgc gctgtttccc ggcgatgtgg accgcctgcg caggatgtct 2281 gtgatcgagg agggggactg caagcggatc aacatggccc acctgtgtgt gattgggtcc 2341 catgctgtca atggtgtggc gaggatccac tcggagatcg tgaaacagtc ggtctttaag 2401 gatttttatg aactggagcc agagaagttc cagaataaga ccaatggcat caccccccgc 2461 cggtggctgc tgctgtgcaa cccggggctg gccgatacca tcgtggagaa aattggggag 2521 gagttcctga ctgacctgag ccagctgaag aagctgctgc cgctggtcag tgacgaggtg 2581 ttcatcaggg acgtggccaa ggtcaaacag gagaacaagc tcaagttctc ggccttcctg 2641 gagaaggagt acaaggtgaa gatcaacccc tcctccatgt tcgatgtgca tgtgaagagg 2701 atccacgagt acaagcggca gctgctcaac tgcctgcacg tcgtcaccct gtacaatcga 2761 atcaagagag acccggccaa ggcttttgtg cccaggactg ttatgattgg gggcaaggca 2821 gcgcccggtt accacatggc caagctgatc atcaagttgg tcacctccat cggcgacgtc 2881 gtcaatcatg acccagttgt gggtgacagg ttgaaagtga tcttcctgga gaactaccgt 2941 gtgtccttgg ctgagaaagt gatcccggcc gctgatctgt cgcagcagat ctccactgca 3001 ggcaccgagg cctcaggcac aggcaacatg aagttcatgc tcaacggggc cctcaccatc 3061 ggcaccatgg acggcgccaa cgtggagatg gccgaggagg ccggggccga gaacctcttc 3121 atcttcggcc tgcgggtgga ggatgtcgag gccttggacc ggaaagggta caatgccagg 3181 gagtactacg accacctgcc cgagctgaag caggccgtgg accagatcag cagtggcttt 3241 ttttctccca aggagccaga ctgcttcaag gacatcgtga acatgctgat gcaccatgac 3301 aggttcaagg tgtttgcaga ctatgaagcc tacatgcagt gccaggcaca ggtggaccag 3361 ctgtaccgga accccaagga gtggaccaag aaggtcatca ggaacatcgc ctgctcgggc 3421 aagttctcca gtgaccggac catcacggag tatgcacggg agatctgggg tgtggagccc 3481 tccgacctgc agatcccgcc ccccaacatc ccccgggact aggaattctg cagatatcca 3541 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact 3601 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 3661 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 3721 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 3781 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 3841 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg 3901 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 3961 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 4021 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 4081 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 4141 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 4201 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 4261 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 4321 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 4381 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 4441 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 4501 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 4561 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 4621 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 4681 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 4741 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 4801 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 4861 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 4921 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 4981 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 5041 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 5101 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 5161 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 5221 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 5281 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 5341 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 5401 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 5461 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 5521 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 5581 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 5641 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 5701 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 5761 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 5821 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 5881 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 5941 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 6001 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 6061 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 6121 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6181 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6241 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 6301 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 6361 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 6421 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 6481 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 6541 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 6601 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 6661 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 6721 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 6781 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 6841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 6901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 6961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7141 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 7321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 7621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 7861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 7921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 7981 aaaagtgcca cctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.