pLV3-CMV-AIFM1-3×FLAG-mCherry-

pLV3-CMV-AIFM1-3×FLAG-mCherry-Puro plasmid

PVT93949

pLV3-CMV-AIFM1-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-AIFM1(human)-3×FLAG-mCherry-Puro
Alias: NM_004208.4;AIF; AUNX1; CMT2D; CMTX4; COWCK; COXPD6;DFNX5; NADMR; NAMSD; PDCD8; SEMDHL
Gene lenght: 1839bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-AIFM1-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-AIFM1-3×FLAG-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-AIFM1-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgttccg gtgtggaggc ctggcggcgg gtgctttgaa 2341 gcagaagctg gtgcccttgg tgcggaccgt gtgcgtccga agcccgaggc agaggaaccg 2401 gctcccaggc aacttgttcc agcgatggca tgttcctcta gaactccaga tgacaagaca 2461 aatggctagc tctggtgcat cagggggcaa aatcgataat tctgtgttag tccttattgt 2521 gggcttatca acagtaggag ctggtgccta tgcctacaag actatgaaag aggatgaaaa 2581 aagatacaat gaaagaattt cagggttagg gctgacacca gaacagaaac agaaaaaggc 2641 cgcgttatct gcttcagaag gagaggaagt tcctcaagac aaggcgccaa gtcatgttcc 2701 tttcctgcta attggtggag gcacagctgc ttttgctgca gccagatcca tccgggctcg 2761 ggatcctggg gccagggtac tgattgtatc tgaagatcct gagctgccgt acatgcgacc 2821 tcctctttca aaagaactgt ggttttcaga tgacccaaat gtcacaaaga cactgcgatt 2881 caaacagtgg aatggaaaag agagaagcat atatttccag ccaccttctt tctatgtctc 2941 tgctcaggac ctgcctcata ttgagaatgg tggtgtggct gtcctcactg ggaagaaggt 3001 agtacagctg gatgtgagag acaacatggt gaaacttaat gatggctctc aaataaccta 3061 tgaaaagtgc ttgattgcaa caggaggtac tccaagaagt ctgtctgcca ttgatagggc 3121 tggagcagag gtgaagagta gaacaacgct tttcagaaag attggagact ttagaagctt 3181 ggagaagatt tcacgggaag tcaaatcaat tacgattatc ggtgggggct tccttggtag 3241 cgaactggcc tgtgctcttg gcagaaaggc tcgagccttg ggcacagaag tgattcaact 3301 cttccccgag aaaggaaata tgggaaagat cctccccgaa tacctcagca actggaccat 3361 ggaaaaagtc agacgagagg gggttaaggt gatgcccaat gctattgtgc aatccgttgg 3421 agtcagcagt ggcaagttac ttatcaagct gaaagacggc aggaaggtag aaactgacca 3481 catagtggca gctgtgggcc tggagcccaa tgttgagttg gccaagactg gtggcctgga 3541 aatagactca gattttggtg gcttccgggt aaatgcagag ctacaagcac gctctaacat 3601 ctgggtggca ggagatgctg catgcttcta cgatataaag ttgggaagga ggcgggtaga 3661 gcaccatgat cacgctgttg tgagtggaag attggctgga gaaaatatga ctggagctgc 3721 taagccgtac tggcatcagt caatgttctg gagtgatttg ggccccgatg ttggctatga 3781 agctattggt cttgtggaca gtagtttgcc cacagttggt gtttttgcaa aagcaactgc 3841 acaagacaac cccaaatctg ccacagagca gtcaggaact ggtatccgat cagagagtga 3901 gacagagtcc gaggcctcag aaattactat tcctcccagc accccggcag ttccacaggc 3961 tcccgtccag ggggaggact acggcaaagg tgtcatcttc tacctcaggg acaaagtggt 4021 cgtggggatt gtgctatgga acatctttaa ccgaatgcca atagcaagga agatcattaa 4081 ggacggtgag cagcatgaag atctcaatga agtagccaaa ctattcaaca ttcatgaaga 4141 cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 4201 TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc 4261 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 4321 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 4381 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 4441 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 4501 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 4561 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 4621 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 4681 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 4741 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga 4801 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 4861 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 4921 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 4981 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 5041 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 5101 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 5161 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 5221 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 5281 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 5341 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 5401 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 5461 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct 5521 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 5581 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 5641 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 5701 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 5761 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 5821 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 5881 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 5941 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 6001 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 6061 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 6121 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 6181 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 6241 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 6301 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 6361 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 6421 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 6481 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 6541 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 6601 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 6661 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 6721 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 6781 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 6841 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 6901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 6961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 7021 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 7081 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 7141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7201 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 7261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 7321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 7381 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 7441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 7501 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 7561 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 7621 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 7681 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 7741 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 7801 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 7861 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 7921 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 7981 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 8041 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 8101 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 8161 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 8221 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 8281 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 8341 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 8401 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 8461 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 8521 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 8581 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 8641 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 8701 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 8761 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 8821 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 8881 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 8941 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 9001 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 9061 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 9121 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 9181 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 9241 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 9301 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 9361 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 9421 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 9481 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 9541 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 9601 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 9661 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 9721 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 9781 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 9841 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 9901 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 9961 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 10021 cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.