pTac-HIF1A-MBP plasmid

pTac-HIF1A-MBP plasmid

PVT93948

pTac-HIF1A-MBP plasmid Information

Plasmid name: pTac-HIF1A(human)-MBP
Alias: NM_001530.4;bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha;HIF1; HIF1-ALPHA; MOP1; PASD8
Gene lenght: 2478bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pQE30-F
Reverse primer:
Copy number: High
Induction: IPTG Induction

pTac-HIF1A-MBP plasmid Description

pTac-HIF1A-MBP is a plasmid suitable for protein expression in Escherichia coli.

pTac-HIF1A-MBP plasmid Sequence

1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1501 gcacttcacc aacaaggacc atagcatatg gagggcgccg gcggcgcgaa cgacaagaaa 1561 aagataagtt ctgaacgtcg aaaagaaaag tctcgagatg cagccagatc tcggcgaagt 1621 aaagaatctg aagtttttta tgagcttgct catcagttgc cacttccaca taatgtgagt 1681 tcgcatcttg ataaggcctc tgtgatgagg cttaccatca gctatttgcg tgtgaggaaa 1741 cttctggatg ctggtgattt ggatattgaa gatgacatga aagcacagat gaattgcttt 1801 tatttgaaag ccttggatgg ttttgttatg gttctcacag atgatggtga catgatttac 1861 atttctgata atgtgaacaa atacatggga ttaactcagt ttgaactaac tggacacagt 1921 gtgtttgatt ttactcatcc atgtgaccat gaggaaatga gagaaatgct tacacacaga 1981 aatggccttg tgaaaaaggg taaagaacaa aacacacagc gaagcttttt tctcagaatg 2041 aagtgtaccc taactagccg aggaagaact atgaacataa agtctgcaac atggaaggta 2101 ttgcactgca caggccacat tcacgtatat gataccaaca gtaaccaacc tcagtgtggg 2161 tataagaaac cacctatgac ctgcttggtg ctgatttgtg aacccattcc tcacccatca 2221 aatattgaaa ttcctttaga tagcaagact ttcctcagtc gacacagcct ggatatgaaa 2281 ttttcttatt gtgatgaaag aattaccgaa ttgatgggat atgagccaga agaactttta 2341 ggccgctcaa tttatgaata ttatcatgct ttggactctg atcatctgac caaaactcat 2401 catgatatgt ttactaaagg acaagtcacc acaggacagt acaggatgct tgccaaaaga 2461 ggtggatatg tctgggttga aactcaagca actgtcatat ataacaccaa gaattctcaa 2521 ccacagtgca ttgtatgtgt gaattacgtt gtgagtggta ttattcagca cgacttgatt 2581 ttctcccttc aacaaacaga atgtgtcctt aaaccggttg aatcttcaga tatgaaaatg 2641 actcagctat tcaccaaagt tgaatcagaa gatacaagta gcctctttga caaacttaag 2701 aaggaacctg atgctttaac tttgctggcc ccagccgctg gagacacaat catatcttta 2761 gattttggca gcaacgacac agaaactgat gaccagcaac ttgaggaagt accattatat 2821 aatgatgtaa tgctcccctc acccaacgaa aaattacaga atataaattt ggcaatgtct 2881 ccattaccca ccgctgaaac gccaaagcca cttcgaagta gtgctgaccc tgcactcaat 2941 caagaagttg cattaaaatt agaaccaaat ccagagtcac tggaactttc ttttaccatg 3001 ccccagattc aggatcagac acctagtcct tccgatggaa gcactagaca aagttcacct 3061 gagcctaata gtcccagtga atattgtttt tatgtggata gtgatatggt caatgaattc 3121 aagttggaat tggtagaaaa actttttgct gaagacacag aagcaaagaa cccattttct 3181 actcaggaca cagatttaga cttggagatg ttagctccct atatcccaat ggatgatgac 3241 ttccagttac gttccttcga tcagttgtca ccattagaaa gcagttccgc aagccctgaa 3301 agcgcaagtc ctcaaagcac agttacagta ttccagcaga ctcaaataca agaacctact 3361 gctaatgcca ccactaccac tgccaccact gatgaattaa aaacagtgac aaaagaccgt 3421 atggaagaca ttaaaatatt gattgcatct ccatctccta cccacataca taaagaaact 3481 actagtgcca catcatcacc atatagagat actcaaagtc ggacagcctc accaaacaga 3541 gcaggaaaag gagtcataga acagacagaa aaatctcatc caagaagccc taacgtgtta 3601 tctgtcgctt tgagtcaaag aactacagtt cctgaggaag aactaaatcc aaagatacta 3661 gctttgcaga atgctcagag aaagcgaaaa atggaacatg atggttcact ttttcaagca 3721 gtaggaattg gaacattatt acagcagcca gacgatcatg cagctactac atcactttct 3781 tggaaacgtg taaaaggatg caaatctagt gaacagaatg gaatggagca aaagacaatt 3841 attttaatac cctctgattt agcatgtaga ctgctggggc aatcaatgga tgaaagtgga 3901 ttaccacagc tgaccagtta tgattgtgaa gttaatgctc ctatacaagg cagcagaaac 3961 ctactgcagg gtgaagaatt actcagagct ttggatcaag ttaaccatat gaaaatcgaa 4021 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4081 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4141 gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4201 cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4261 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4321 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4381 aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4441 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4501 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4561 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4621 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4681 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4741 gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4801 gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4861 ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4921 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4981 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5041 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5101 gcgcagactt gaAAGCTTGG cactggccgt cgttttacaa cgtcgtgact gggaaaaccc 5161 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag 5221 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca 5281 gcttggctgt tttggcggat gagataagat tttcagcctg atacagatta aatcagaacg 5341 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga 5401 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca 5461 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 5521 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg 5581 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat 5641 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc 5701 tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 5761 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 5821 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 5881 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 5941 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttctccaat 6001 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca 6061 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 6121 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 6181 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 6241 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 6301 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 6361 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 6421 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 6481 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 6541 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 6601 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 6661 gtaactgtca gaccaagttt actcatatat actttagatt gatttacccc ggttgataat 6721 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat 6781 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 6841 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 6901 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 6961 accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg 7021 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 7081 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 7141 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 7201 gcgccgctac agggcgcgta aaaggatcta ggtgaagatc ctttttgata atctcatgac 7261 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 7321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 7381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 7441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 7501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 7561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 7621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 7681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 7741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 7801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 7861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 7921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 7981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 8041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 8101 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 8161 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat 8221 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 8281 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 8341 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct 8401 catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 8461 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg 8521 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa 8581 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc 8641 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa 8701 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 8761 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg 8821 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag 8881 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac 8941 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca 9001 cccgtggcca ggacccaacg ctgcccgaaa tt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.