pCMV-Usp11-Neo plasmid

pCMV-Usp11-Neo plasmid

PVT93697

pCMV-Usp11-Neo plasmid Information

Plasmid name: pCMV-Usp11(mouse)-Neo
Alias: NM_145628.4;6230415D12Rik; mKIAA4085
Gene lenght: 2766bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Usp11-Neo plasmid Description

pCMV-Usp11-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Usp11-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CGCCACCatg gcggcggtcg cagcggaccc agctgcagct gccgtcccgg cctctgctga 961 ggaccgagac acacagcccg aggcaatgcc agacctggat gaacagtggc gccaaatcgg 1021 gaatgggcga gagcgtccac tgcgagctgg cgaaagctgg ttccttgtgg agaaacactg 1081 gtataaacag tgggaggtgt acgtgaaggg aggggatcaa gatgctagca cctttcctgg 1141 ctgtatcaac aatgctgggc tctttgaaga tcagataagc tggcatctca gggagagact 1201 gctagaagga gatgattatg tgctgctccc agcgcctgct tggaactaca tggtcagctg 1261 gtatggctta atggatggcc agccacctat tgagcgcaag gtaatagaac ttcctggcat 1321 tcggaaggtg gaagtgtacc cactagagct actgctcgtt cagcacagtg atatggaaac 1381 agctctcacc attcagttta gctatactga ttctgtggaa ctagtcttgc aaacagctcg 1441 ggagcagttt ctggtagagc ctcaggaaga cacgcgcctc tggaccaaga actcagaggg 1501 ctctttggat cgactgtgta atacacagat cacgctgctt gatgcctgcc ttgagactgg 1561 gcagttggtc atcatggaga ctcgaaacaa agatggcact tggcccagtg ctcagctgtg 1621 tggcatgaac aacataccgg acgaggatga ggacttccag gggcaaccgg gcatctgtgg 1681 ccttaccaat ctgggcaaca catgcttcat gaactcggcc ctacagtgcc tgagcaatgt 1741 gccacagctg acagagtact ttctcaacaa tcgctaccta gaggagctca acttccggaa 1801 ccctctaggc atgaagggtg agcttgctga ggcctatgca gatctggtaa agcagacttg 1861 gtctggctac caccgctcca ttgtgccaaa tgtgttcaag aacaaggttg gccattttgc 1921 atcccagttt ctgggctacc agcagcatga ctcacaggaa ctactgtcat tcctcctgga 1981 tgggctacat gaggatctca atcgggtcaa aaagaaagaa tatgttgagc tatgcaatgg 2041 tgctgggcgg cctgatctgg aagtggctca ggaagcctgg cagaaccata aacgacgaaa 2101 tgattctgtg atcgtggaca ctttccatgg cctcttcaag tccaccctgg tgtgccctga 2161 ttgtggcaat gtttctgtga cctttgaccc cttctgctac ctcagtgtcc cactgcctgt 2221 ctgctccagg agggtcttgg aagtcttctt tgtccccatg gatccccgcc gcaagccaga 2281 acagcaccgg gttgtagtcc ccaagaaggg caatatctca gatctgtgtg tggctctgtc 2341 cacgcataca agtgttgcac cagacaagat gatagtagct gatgtcttca gtcaccgatt 2401 ctataagctt taccagctgg aggatcctct gagtggcatc ttggatcgtg atgatatctt 2461 tgtatatgag gtgactggtc ggattgagcc ggttgagggt tcaagagatg atattgtggt 2521 acctgtttac ttgcgggagc gcaccccatc cagagactac aacaactcct actatggtct 2581 gatacttttt gggcaccctc tcctggtgtc agtgccccgg gaccggttct cctgggaggg 2641 cctatataac atcttgatgt accgactttc acgatatgtg accaaaccca cctcagatga 2701 ggatgatggg gatgagaaag ttgatgaaga tgaggatgag gatgtggaag atgacagcag 2761 cagtgaggag gaaaaggagg aaatgtctgc gcccactgtt aatgatggga cccgagaagc 2821 agagcaggag caggctggga ccagctctgg ggtcactgag agatgcccat ccctcttaga 2881 caacagcctc cgcgcatcgc agtggccccc aaggcgacga cgaaagcagc tgtttactct 2941 gcagacggtg aactccaatg gaaccagtga ccggaccact tcccctgaag aaatgcaaac 3001 ccagccatac attgccatgg attgggaacc agatatgaag aggcgttatt atgacgaagt 3061 ggaggctgag ggctatgtga agcatgactg tgtgggctac atgctgaaga aaagtccagt 3121 gcagctgaag gagtgcatca aactcttcac cactgtggaa accctggaga aggagaaccc 3181 ctggtactgc tcctcctgca agcagcacca gctggccacc aagaaactgg acctctggat 3241 gctacccgaa gttctcatta tccacctgaa gcgtttttca ttttccaaaa tttcccggga 3301 gaagctggac accctggtgc agtttcctat ccgggacctg gatttctctg agtttgtcat 3361 caaaccaaag aatgagtctt ctccagacct ctacaaatat gatctcatcg cagtttccaa 3421 ccattatggt ggcatgcgtg atggacacta tacaacattt gcctgcaaca aggacagtgg 3481 ccagtggcac tactttgatg acaatagtgt ctcacctgtg aatgagaatc agattgagtc 3541 caaggcagcc tatgtcttgt tctatcagcg ccaagatgtg ggtcgacgcc agtcccagac 3601 ttcttcatct gacactcctg cctctcctgt ctccagttct acacccaact ctgacatcat 3661 ggatatcaac taaCTCGAGT CTAGAGGGCC CGTTTAAACC CGCTGATCAG CCTCGACTGT 3721 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA 3781 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG 3841 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA 3901 AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC 3961 CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 4021 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 4081 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 4141 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 4201 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 4261 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 4321 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 4381 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA 4441 GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT 4501 TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 4561 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA 4621 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA 4681 GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA 4741 GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA 4801 GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG 4861 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT 4921 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC 4981 CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG 5041 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG 5101 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA 5161 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA 5221 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT 5281 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC 5341 AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC 5401 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG 5461 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT 5521 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG 5581 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA 5641 TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT 5701 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG 5761 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT 5821 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA 5881 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA 5941 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA 6001 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG 6061 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT 6121 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 6181 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 6241 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 6301 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 6361 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 6421 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 6481 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 6541 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 6601 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 6661 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 6721 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 6781 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 6841 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 6901 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 6961 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 7021 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 7081 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 7141 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 7201 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 7261 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 7321 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 7381 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG 7441 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT 7501 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 7561 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC 7621 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA 7681 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC 7741 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT 7801 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 7861 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA 7921 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA 7981 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT 8041 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA 8101 AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT CCCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.