pCMV-Ltbp2-3×FLAG-Neo plasmid

pCMV-Ltbp2-3×FLAG-Neo plasmid

PVT93691

pCMV-Ltbp2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Ltbp2(rat)-3×FLAG-Neo
Alias: NM_021586.2
Gene lenght: 5361bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pCMV-Ltbp2-3×FLAG-Neo plasmid Description

pCMV-Ltbp2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Ltbp2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCg ccaccATGGA GAGCACCTCC CTGCGAGGTC 781 TCCGGTGCCC ACAGCTCTGC AGCCACTCTG GCGCCATGAG GGCGCCGACC ACCGTCCGCT 841 GCTCCGGACG CATCCAAAGG GCGCGTTGGA GGGGCTTCCT GCCACTTGTC CTGGCTCTCT 901 TGATGGGGAC AAGTCATGCC CAAAGGGATT CCGTGGGGAG ATACGAACCA GCTAGCCGGG 961 ATGCCAATCG GTTGTGGCGC CCCGTGGGCA ACCACCCCGC AGCGGCTGCA GCCAAGGTGT 1021 ACAGTCTGTT CCGAGAGCCC GACGCGCCGG TCCCCGGCTT GTCGCCCTCT GAGTGGAATC 1081 AGCCGGGCCA GGGGATCCCT GGGAGGCTCG CAGAGGCCGA GGCCAGGAGA CCGTCCCGAG 1141 CCCAGCAGCT GCGTCGAGTC CAGTCACCTG TCCAGACTCG GAGAAGCAAT CCCCGAGGCC 1201 AGCAGCCACC AGCAGCCCGG ACCGCACATT CCGTCGTGCG CCTGGCGACC CCTCAGCGAC 1261 CCGCGGCTGC ACGCCGAGGG CGGCTCACCG GGAGAAATGT CTGCGGGGGA CAGTGCTGCC 1321 CTGGATGGAC GACATCGAAC AGCACCAACC ACTGTATCAA ACCTGTGTGT CAGCCTCCCT 1381 GTCAGAACCG GGGCTCCTGC AGCCGGCCCC AGCTCTGCAT CTGCCGTTCT GGCTTCCGTG 1441 GGGCACGCTG CGAGGAGGTC ATCCCTGAGG AGGAGTTTGA CCCTCAGAAT GCCAGGCCTG 1501 TGCCCAGACG CTCAGTGGAG GGAGCACCTG GCCCTCACAG GAGCAGCGAG GCCAGAGGAA 1561 GTCTAGTGAC CAGAATACAG CCGCTGCTAC CACCACTACC ACCACCTCCA TCTAGGACCC 1621 TCAGCCAGAC CCGTCCCCTG CAGCAGCATG CAGGACTGTC CAGAACAGTT CGTCGTTATC 1681 CGGCCACTGG TACCAATGGC CAACTGATGT CCAACGCTCT GCCTTCAGGA CCAGGACCTG 1741 AGCTGAGAGA CAGCAGCCAA CAGGCAGCAC ACATGAACCA TCTCTCACAC CCCTGGGGGC 1801 TGAACCTCAC CGAGAAAATC AAGAAGATTA AGGTCGTCTT CACTCCCACC ATCTGCAAGC 1861 AGACCTGTGC CCGGGGCCGC TGTGCCAACA CGTGTGAGAA GGGTGACACC ACCACCCTGT 1921 ACAGTCAGGG CGGCCATGGG CATGACCCCA AGTCTGGCTT CCGTATCTAT TTCTGCCAAA 1981 TCCCCTGCCT GAATGGAGGC CGCTGCATTG GCCGGGACGA GTGCTGGTGT CCAGCCAACT 2041 CTACAGGGAA GTTCTGCCAT CTGCCTGTCC CACAGCCAGA CAGGGAGCCT CCAGGACGAG 2101 GCTCCCAGCA CAGAGCCCTG CTGGAAGGGC CATTGAAGCA ATCCACCTTC ACGCTGCCTC 2161 TCTCCAACCA GCTGGCCTCT GTGAACCCCT CGCTGGTGAA GGTACAAATG CAGCACCCGC 2221 CTGAGGCCTC CGTGCAGATC CACCAGGTGG CCCGGGTCCG GGGTGAGGTG GACCCTGTGC 2281 CAGAGGACAA CAGTGTGGAG ACCAGAGCCT CTCATCGCCC CCATGGCAGC TCAGGCCACA 2341 GCCACTGGGC CAGCAACAGC ATACCCGCTC GGGCTGGAGA GGCCCCTCGG CCACCACCAG 2401 TGCCGTCCAG GCATTATGGA CTTCTGGGCC AGTGTTACCT GAGCACGGTG AATGGACAGT 2461 GTGCTAACCC CCTAGGGGAG CTGACTTCTC AGGAAGACTG CTGTGGCAGT GTGGGGACTT 2521 CTTGGGGGGT GACTTCCTGT GCCCCATGCC CACCCAGACC AGCTTTCCCC GTGATTGAAA 2581 ACGGCCAGCT GGAGTGTCCC CAAGGGTATA AGAGACTAAA CCTCAGCCAT TGCCAAGACA 2641 TCAATGAGTG CCTGACCCTG GGCCTGTGCA AGGATTCAGA GTGTGTGAAC ACCAGGGGCA 2701 GCTACCTGTG CACCTGCAGG CCCGGCCTCA TGCTGGATCC ATCAAGGAGC CGCTGTGTAT 2761 CGGACAAGGC TGTCTCCATG AAACAGGGAC TCTGTTACCG GTCAATGGTG TCTGGCACCT 2821 GCACCCTGCC TTTGGTACAA CGGATCACCA AGCAGATATG CTGTTGCAGC CGTGTGGGCA 2881 AAGCCTGGGG CAGCAAATGT GAACACTGCC CCCTGCCTGG CACAGAAGCC TTCAGGGAGA 2941 TCTGCCCTGC TGGCCATGGC TACGCCTACT CAAGCTCAGA CATCCGCCTG TCTATGAGGA 3001 AAGCTGAGGA AGAGGAACTG GCTAGCCCCG TAAGGGAACA GAGACAGCAG AGCAGTGGAC 3061 CCCCACCTGG GGCAGCAGAA AGGCAGCCAC TCCGGGCAGC CACTGCCACC TGGATTGAGG 3121 CTGAGACCCT CCCTGACAAA GGTGACTCTC GGGCTATTCA GATTACAACC AGTGCTCCCC 3181 ACCTACCTGC CCGGGTACCA GGGGATGCCA CTGGAAGACC AACGCCATCA TTGCCTGGAC 3241 AGGGCATTCC AGAGGGTCCA GCAGAAGAGC AGGTGATCCC TTCCAGTGAT GTCCTGGTGA 3301 CGCACGGTCC CCCAGGCTTT GATCCATGTT TCGCTGGAGC CTCCAACATC TGTGGCCCTG 3361 GGACCTGTGT GAAGCTCCCA AATGGATACA GATGTGTCTG CAGCCCTGGT TACCAGCTAC 3421 ACCCCAGCCA GGACTACTGT ACTGATGACA ACGAGTGTCT GAGGAACCCC TGTGAAGGAA 3481 GAGGGCGCTG TGTCAACAGT GTGGGCTCCT ACTCCTGCCT CTGCTACCCA GGCTACACAC 3541 TAGCCACCCT AGGAGACACA CAGGAGTGCC AAGATGTGGA TGAGTGTGAG CAGCCGGGGG 3601 TGTGCAGCGG TGGACGATGC AGCAACACTG AGGGCTCGTA CCACTGCGAG TGTGATCAGG 3661 GCTACGTCAT GGTCAGAAGA GGACACTGCC AAGATATCAA CGAATGCCGT CACCCTGGTA 3721 CCTGCCCTGA TGGGAGATGC GTCAACTCCC CTGGCTCCTA CACTTGTCTG GCCTGTGAGG 3781 AGGGCTACAT AGGGCAGAGC GGGAACTGTG TAGATATGAA TGAGTGTCTG ACCCCCGGGA 3841 TATGTGCCCA TGGAAGGTGC ATCAACATGG AAGGCTCCTT TAGATGCTCT TGTGAGCCAG 3901 GCTATGAGCT CACCCCAGAC AAGAAGGGCT GCCGAGATGT GGACGAGTGT GCCAGCCGAG 3961 CCTCATGCCC CACCGGCCTC TGCCTCAACA CGGAGGGCTC CTTCACCTGC TCAGCCTGTC 4021 AGAGTGGGTA CTGGGTGAAC GAAGATGGCA CTGCCTGTGA AGACCTGGAT GAATGTGCCT 4081 TCCCCGGAGT CTGCCCCACA GGCGTCTGCA CCAACACTGT GGGCTCCTTC TCCTGCAAGG 4141 ACTGCGACAG GGGCTTCCGG CCCAGCCCCC TGGGCAACAG CTGTGAAGAT GTGGATGAGT 4201 GTGAAGGTCC CCAGAACAGC TGCCTGGGAG GCGAGTGCAA GAACACAGAT GGTTCCTACC 4261 AGTGCCTCTG TCCCCAGGGC TTCCAGCTGG CCAATGGCAC CGTGTGTGAG GATGTGGACG 4321 AATGTGTTGG GGAAGAACAC TGCGCTCCTC ATGGCGAATG CCTCAACAGC CCGGGGTCCT 4381 TCTTCTGTCT CTGTGCACCC GGCTTTGCTA GTGCTGAGGG GGGCACCAGA TGCCAGGATG 4441 TTGATGAATG TGCAACCACA GAGCCGTGTC TGGGAGGACA CTGTGTCAAC ACCGAGGGCT 4501 CCTTCAACTG TCTGTGTGAG ACTGGCTTCC AGCCCGCCCC AGACAGTGGA GAGTGTGTGG 4561 ACATAGATGA ATGTGCAAAT GATACTGTGT GTGGGAACCA TGGCTTCTGT GACAATACGG 4621 ATGGCTCCTT CCGCTGCCTG TGTGACCAGG GCTTCGAGAC CTCACCCTCA GGCTGGGAGT 4681 GTGTTGATGT GAACGAGTGT GAGCTCATGC TGGCAGTGTG TGGGGATGCA CTCTGCGAGA 4741 ACGTGGAAGG CTCCTTCCTG TGCCTTTGTG CCAGTGACCT TGAGGAGTAT GATGCAGAAG 4801 AAGGACACTG CCGTCCTCGG GTGGCTGGAG CTCAGAGAAT CCCAGAGGTC CCAACAGAGG 4861 AGCAGGCTGC AGGCCTTACC GGCATGGAGT GCTATGCTGA ACACAATGGT GGTCCTCCAT 4921 GCTCTCAAAT CTTGGGCCAG AACTCCACAC AGGCTGAGTG CTGCTCGACC CAGGGTGCCA 4981 GATGGGGGGA AACCTGTGAT CCCTGCCCAT CTGAGGACTC AGTTGAATTC AGTGAGCTGT 5041 GCCCCAGTGG TCAAGGTTAC ATCCCAGTGG AAGGGGCCTG GACATTTGGA CAAGCCATGT 5101 ATACAGATGC CGACGAGTGC ATACTGTTTG GGCCTGCTCT CTGCCAGAAT GGCCGATGCC 5161 TCAACACAGT GCCTGGCTAC ATTTGCCTGT GCAACCCTGG CTACCACTAT GATGCCGTCA 5221 GCAGGAAGTG CCAGGATCAC AACGAATGCC AGGACTTGGC CTGTGAGAAC GGCGAGTGTG 5281 TGAACACAGA AGGCTCCTTC CACTGCTTCT GCAGTCCCCC CCTCATCCTA GACCTCAGCG 5341 GACAGCGCTG TGTGAACAGT ACCAGCAGCT CAGAGGACTT CCCTGACCAT GACATCCACA 5401 TGGACATCTG CTGGAAAAAA GTCACCAATG ACGTGTGCAG CCAGCCCTTG CGTGGGCACC 5461 ATACTACCTA TACAGAGTGC TGCTGCCAAG ACGGGGAGGC CTGGAGCCAG CAGTGTGCTC 5521 TGTGCCCCCC CAGGAGCTCT GAGGTCTATG CTCAGCTGTG CAATGTGGCT CGGATTGAGG 5581 CAGAGAGGGA AGCAGGGATC CACTTCCGGC CAGGATATGA GTATGGCCCT GGCCCAGATG 5641 ATCTACCTGA AACCCTCTAC GGCCCAGATG GAGCCCCTTT CTATAACTAC CTGGGCCCTG 5701 AGGACACTGT TCCTGAGCCT CCCTTCTCCA ACACAGCCAG TCATTTGGGA GACAACACAC 5761 CCATCCTTGA GCCTCCTCTG CAGCCCTCTG AACTTCAGCC CCACTATATC CCCAGCCATT 5821 CAGAACCCCT GGCTTCCTTC GAAGGCCTTC AGGCTGAGGA ATGTGGCATC CTGAATGGCT 5881 GTGAGAATGG CCGCTGTGTG CGTGTGCGCG AGGGCTACAC TTGTGACTGC TTTGAAGGCT 5941 TCCAGCTGGA TACAGCCCTC ATGGCCTGTG TGGATGTGAA TGAGTGTGAA GACCTGAACG 6001 GGCCTGCGGC ACTCTGTGCG CATGGTCACT GCGAGAACAC AGAGGGTTCC TATCGCTGCC 6061 ACTGTTCCCC TGGTTACGTG GCAGAGCCCG GGCCCCCACA CTGTGCAGCC AAGGAGGGAT 6121 CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG 6181 ATGACAAGTA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA 6241 AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT 6301 AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA 6361 ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT 6421 GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT 6481 CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG 6541 CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC 6601 GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG 6661 GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT 6721 GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC 6781 CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA 6841 ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT 6901 TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC 6961 ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC 7021 AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC 7081 AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG 7141 CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC 7201 CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC 7261 TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA 7321 AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG 7381 CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC 7441 ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA TTTCGGCCTA 7501 TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG 7561 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 7621 CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT 7681 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC 7741 CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT 7801 ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 7861 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 7921 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 7981 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 8041 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 8101 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 8161 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 8221 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 8281 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 8341 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 8401 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 8461 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 8521 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 8581 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 8641 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 8701 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 8761 GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 8821 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT 8881 CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC 8941 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 9001 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC 9061 GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 9121 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 9181 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 9241 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 9301 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 9361 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 9421 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 9481 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 9541 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 9601 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 9661 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT 9721 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 9781 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 9841 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 9901 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 9961 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 10021 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 10081 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 10141 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 10201 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 10261 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 10321 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 10381 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 10441 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 10501 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 10561 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 10621 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 10681 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 10741 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 10801 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 10861 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 10921 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 10981 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 11041 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 11101 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 11161 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 11221 CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA 11281 TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT 11341 GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA 11401 GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.