pTac-GST-SERINC2 plasmid

pTac-GST-SERINC2 plasmid

PVT93541

pTac-GST-SERINC2 plasmid Information

Plasmid name: pTac-GST-SERINC2(human)
Alias: NM_178865.5;FKSG84; PRO0899; TDE2; TDE2L
Gene lenght: 1368bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-SERINC2 plasmid Description

pTac-GST-SERINC2 is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-SERINC2 plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggg ggcctgcctg ggagcctgct 961 ccctgctcag ctgcgcgtcc tgcctctgcg gctctgcccc ctgcatcctg tgcagctgct 1021 gccccgccag ccgcaactcc accgtgagcc gcctcatctt cacgttcttc ctcttcctgg 1081 gggtgctggt gtccatcatt atgctgagcc cgggcgtgga gagtcagctc tacaagctgc 1141 cctgggtgtg tgaggagggg gccgggatcc ccaccgtcct gcagggccac atcgactgtg 1201 gctccctgct tggctaccgc gctgtctacc gcatgtgctt cgccacggcg gccttcttct 1261 tctttttcac cctgctcatg ctctgcgtga gcagcagccg ggacccccgg gctgccatcc 1321 agaatgggtt ttggttcttt aagttcctga tcctggtggg cctcaccgtg ggtgccttct 1381 acattcctga cggctccttc accaacatct ggttctactt cggcgtcgtg ggctccttcc 1441 tcttcatcct catccagctg gtgctgctca tcgactttgc gcactcctgg aaccagcggt 1501 ggctgggcaa ggccgaggag tgcgattccc gtgcctggta cgcaggcctc ttcttcttca 1561 ctctcctctt ctacttgctg tcgatcgcgg ccgtggcgct gatgttcatg tactacactg 1621 agcccagcgg ctgccacgag ggcaaggtct tcatcagcct caacctcacc ttctgtgtct 1681 gcgtgtccat cgctgctgtc ctgcccaagg tccaggacgc ccagcccaac tcgggtctgc 1741 tgcaggcctc ggtcatcacc ctctacacca tgtttgtcac ctggtcagcc ctatccagta 1801 tccctgaaca gaaatgcaac ccccatttgc caacccagct gggcaacgag acagttgtgg 1861 caggccccga gggctatgag acccagtggt gggatgcccc gagcattgtg ggcctcatca 1921 tcttcctcct gtgcaccctc ttcatcagtc tgcgctcctc agaccaccgg caggtgaaca 1981 gcctgatgca gaccgaggag tgcccaccta tgctagacgc cacacagcag cagcagcagg 2041 tggcagcctg tgagggccgg gcctttgaca acgagcagga cggcgtcacc tacagctact 2101 ccttcttcca cttctgcctg gtgctggcct cactgcacgt catgatgacg ctcaccaact 2161 ggtacaagcc cggtgagacc cggaagatga tcagcacgtg gaccgccgtg tgggtgaaga 2221 tctgtgccag ctgggcaggg ctgctcctct acctgtggac cctggtagcc ccactcctcc 2281 tgcgcaaccg cgacttcagc tgactcgagc ggccgcatcg tgactgactg acgatctgcc 2341 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 2401 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 2461 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtataatt 2521 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa 2581 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt 2641 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc 2701 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc 2761 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa 2821 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg 2881 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag 2941 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc 3001 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta 3061 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg 3121 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca 3181 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac 3241 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat 3301 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg 3361 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata 3421 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta 3481 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa 3541 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag 3601 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 3661 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 3721 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 3781 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 3841 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 3901 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 3961 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 4021 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 4081 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 4141 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 4201 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 4261 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 4321 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 4381 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 4441 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 4501 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 4561 tgtgcggtat ttcacaccgc ataaattccg acaccatcga atggtgcaaa acctttcgcg 4621 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa 4681 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga 4741 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc 4801 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg 4861 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat 4921 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg 4981 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta 5041 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg 5101 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag 5161 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt 5221 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc 5281 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg 5341 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg 5401 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg 5461 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc 5521 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc 5581 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga 5641 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 5701 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 5761 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 5821 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat 5881 gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 5941 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 6001 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg 6061 gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga 6121 tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa 6181 cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg 6241 ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat 6301 ttttgatggc gttggaatt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.