pCMV-NFKB1-3×HA-Neo plasmid

pCMV-NFKB1-3×HA-Neo plasmid

PVT93425

pCMV-NFKB1-3×HA-Neo plasmid Information

Plasmid name: pCMV-NFKB1(human)-3×HA-Neo
Alias: NM_001382627.1;CVID12; EBP-1; KBF1; NF-kappa-B1;NF-kappaB; NF-kappabeta; NF-kB; NF-kB1; NFkappaB;NFKB-p105; NFKB-p50;
Gene lenght: 2904bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-NFKB1-3×HA-Neo plasmid Description

pCMV-NFKB1-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-NFKB1-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCgccacc atggcagaag atgatccata tttgggaagg 961 cctgaacaaa tgtttcattt ggatccttct ttgactcata caatatttaa tccagaagta 1021 tttcaaccac agatggcact gccaacagat ggcccatacc ttcaaatatt agagcaacct 1081 aaacagagag gatttcgttt ccgttatgta tgtgaaggcc catcccatgg tggactacct 1141 ggtgcctcta gtgaaaagaa caagaagtct taccctcagg tcaaaatctg caactatgtg 1201 ggaccagcaa aggttattgt tcagttggtc acaaatggaa aaaatatcca cctgcatgcc 1261 cacagcctgg tgggaaaaca ctgtgaggat gggatctgca ctgtaactgc tggacccaag 1321 gacatggtgg tcggcttcgc aaacctgggt atacttcatg tgacaaagaa aaaagtattt 1381 gaaacactgg aagcacgaat gacagaggcg tgtataaggg gctataatcc tggactcttg 1441 gtgcaccctg accttgccta tttgcaagca gaaggtggag gggaccggca gctgggagat 1501 cgggaaaaag agctaatccg ccaagcagct ctgcagcaga ccaaggagat ggacctcagc 1561 gtggtgcggc tcatgtttac agcttttctt ccggatagca ctggcagctt cacaaggcgc 1621 ctggaacccg tggtatcaga cgccatctat gacagtaaag cccccaatgc atccaacttg 1681 aaaattgtaa gaatggacag gacagctgga tgtgtgactg gaggggagga aatttatctt 1741 ctttgtgaca aagttcagaa agatgacatc cagattcgat tttatgaaga ggaagaaaat 1801 ggtggagtct gggaaggatt tggagatttt tcccccacag atgttcatag acaatttgcc 1861 attgtcttca aaactccaaa gtataaagat attaatatta caaaaccagc ctctgtgttt 1921 gtccagcttc ggaggaaatc tgacttggaa actagtgaac caaaaccttt cctctactat 1981 cctgaaatca aagataaaga agaagtgcag aggaaacgtc agaagctcat gcccaatttt 2041 tcggatagtt tcggcggtgg tagtggtgct ggagctggag gcggaggcat gtttggtagt 2101 ggcggtggag gagggggcac tggaagtaca ggtccagggt atagcttccc acactatgga 2161 tttcctactt atggtgggat tactttccat cctggaacta ctaaatctaa tgctgggatg 2221 aagcatggaa ccatggacac tgaatctaaa aaggaccctg aaggttgtga caaaagtgat 2281 gacaaaaaca ctgtaaacct ctttgggaaa gttattgaaa ccacagagca agatcaggag 2341 cccagcgagg ccaccgttgg gaatggtgag gtcactctaa cgtatgcaac aggaacaaaa 2401 gaagagagtg ctggagttca ggataacctc tttctagaga aggctatgca gcttgcaaag 2461 aggcatgcca atgccctttt cgactacgcg gtgacaggag acgtgaagat gctgctggcc 2521 gtccagcgcc atctcactgc tgtgcaggat gagaatgggg acagtgtctt acacttagca 2581 atcatccacc ttcattctca acttgtgagg gatctactag aagtcacatc tggtttgatt 2641 tctgatgaca ttatcaacat gagaaatgat ctgtaccaga cgcccttgca cttggcagtg 2701 atcactaagc aggaagatgt ggtggaggat ttgctgaggg ctggggccga cctgagcctt 2761 ctggaccgct tgggtaactc tgttttgcac ctagctgcca aagaaggaca tgataaagtt 2821 ctcagtatct tactcaagca caaaaaggca gcactacttc ttgaccaccc caacggggac 2881 ggtctgaatg ccattcatct agccatgatg agcaatagcc tgccatgttt gctgctgctg 2941 gtggccgctg gggctgacgt caatgctcag gagcagaagt ccgggcgcac agcactgcac 3001 ctggctgtgg agcacgacaa catctcattg gcaggctgcc tgctcctgga gggtgatgcc 3061 catgtggaca gtactaccta cgatggaacc acacccctgc atatagcagc tgggagaggg 3121 tccaccaggc tggcagctct tctcaaagca gcaggagcag atcccctggt ggagaacttt 3181 gagcctctct atgacctgga tgactcttgg gaaaatgcag gagaggatga aggagttgtg 3241 cctggaacca cgcctctaga tatggccacc agctggcagg tatttgacat attaaatggg 3301 aaaccatatg agccagagtt tacatctgat gatttactag cacaaggaga catgaaacag 3361 ctggctgaag atgtgaagct gcagctgtat aagttactag aaattcctga tccagacaaa 3421 aactgggcta ctctggcgca gaaattaggt ctggggatac ttaataatgc cttccggctg 3481 agtcctgctc cttccaaaac acttatggac aactatgagg tctctggggg tacagtcaga 3541 gagctggtgg aggccctgag acaaatgggc tacaccgaag caattgaagt gatccaggca 3601 gcctccagcc cagtgaagac cacctctcag gcccactcgc tgcctctctc gcctgcctcc 3661 acaaggcagc aaatagacga gctccgagac agtgacagtg tctgcgacag cggcgtggag 3721 acatccttcc gcaaactcag ctttaccgag tctctgacca gtggtgcctc actgctaact 3781 ctcaacaaaa tgccccatga ttatgggcag gaaggacctc tagaaggcaa aattCTCGAG 3841 TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC 3901 TATCCTTATG ATGTCCCAGA CTACGCAtga GGGCCCGTTT AAACCCGCTG ATCAGCCTCG 3961 ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC 4021 CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT 4081 CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT 4141 TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA 4201 AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG 4261 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 4321 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 4381 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 4441 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 4501 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 4561 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 4621 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC 4681 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 4741 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 4801 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC 4861 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 4921 ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT 4981 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 5041 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT 5101 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 5161 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 5221 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 5281 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 5341 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG 5401 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 5461 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 5521 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT 5581 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG 5641 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC 5701 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG 5761 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT 5821 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT 5881 CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC 5941 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 6001 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 6061 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 6121 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC 6181 CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 6241 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 6301 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 6361 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 6421 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 6481 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 6541 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 6601 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 6661 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 6721 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 6781 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 6841 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 6901 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 6961 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 7021 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 7081 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 7141 TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 7201 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 7261 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 7321 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 7381 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 7441 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 7501 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 7561 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 7621 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 7681 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 7741 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 7801 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 7861 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 7921 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 7981 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 8041 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 8101 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 8161 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 8221 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 8281 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 8341 CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.